Technology for preparing functional dietary fiber through waste bamboo shell enzymolysis

A dietary fiber and functional technology, applied in food preparation, food science, application, etc., can solve problems such as rot and mildew, odor, ecological environment pollution in bamboo shoot production areas, etc., achieve high yield, control cholesterol, and solve environmental problems Effects of pollution and waste of resources

Inactive Publication Date: 2011-07-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] What the present invention aims to solve is the problem that the existing bamboo shoot shells are discarded by the roadside or ditch, rot and mildew, produce stench, and bring serious pollution to the ecological environment of the bamboo shoot production area

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of bamboo shoot shells: recover fresh bamboo shoot shells, select, wash, pulverize (homogenize) or dry at 60-70°C for later use.

[0016] (2) Organic solvent extraction: Weigh 250g of the dry powder slurry (the slurry is dried at 60-70°C, crushed, and the weight of the dry powder passed through a 80-100 mesh sieve), put it in a closed container, and use the organic solvent diethyl ether to seal and extract the slurry. Lipids in bamboo shoot shells are removed, and the amount of organic solvent ether added is based on submerging the bamboo shoot shells. After leaching for 24 hours, the organic solvent is recovered by pressure filtration, and air-dried or oven-dried to remove residual organic solvents in the bamboo shoot shells.

[0017] (3) Cellulase enzymatic hydrolysis: in the degreased bamboo shoot shells, add 17 mL / g of phosphate buffer solution (dry weight of bamboo shoot shells) with pH=5 and fibers with an enzyme concentration of 1% and an enzyme ac...

Embodiment 2

[0026] (1) Preparation of bamboo shoot shells: recover fresh bamboo shoot shells, dry and crush them at 60°C, pass through 80-mesh sieves, and set aside.

[0027] (2) Organic solvent extraction: Weigh 300g of dry powder slurry, put it in a closed container, seal and extract with organic solvent ether, and remove the lipids in the bamboo shoot shells. The amount of organic solvent ether added is based on submerged bamboo shoot shells. After extracting for 24 hours, the organic solvent is recovered by pressure filtration, air-dried or oven-dried to remove the residual organic solvent in the bamboo shoot shell.

[0028] (3) Cellulase enzymatic hydrolysis: in the degreased bamboo shoot shells, add 20 mL / g of phosphate buffer solution (dry weight of bamboo shoot shells) with pH=4.5 and 0.5% enzyme concentration and 8 u / mL enzyme activity. Glycanase solution, at 120rpm, in a 60°C water bath for 2 hours, then raised the temperature to 100°C, and kept for 5 minutes to inactivate the e...

Embodiment 3

[0036] (1) Bamboo shoot shell preparation: recycle the discarded hairy shoot shells from bamboo shoot processing, dry and pulverize at 60°C, pass through an 80-mesh sieve, and set aside.

[0037] (2) Organic solvent extraction: Weigh 500g of dry powder slurry, put it in a closed container, seal and extract with organic solvent ether, and remove the lipids in the bamboo shoot shells. The amount of organic solvent ether added is based on submerged bamboo shoot shells, After extracting for 24 hours, press filter, dry the bamboo shoot shell, remove the residual organic solvent in the bamboo shoot shell, and recover the organic solvent.

[0038] (3) Cellulase enzymatic hydrolysis: in the degreased bamboo shoot shell, add 20 mL / g of phosphate buffer solution (dry weight of bamboo shoot shell) with pH = 4.5 and fiber with an enzyme concentration of 1% and an enzyme activity of 6u / mL Suzyme solution, at 120rpm, in a 50°C water bath, for 1 hour of enzymatic hydrolysis.

[0039] (4) De...

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PUM

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Abstract

The present invention discloses a method for enzymolyzing waste bamboo shoot coats to prepared functional diet fiber. Waste bamboo shoot coats, after being washed, dried, crushed and defatted, is added into pH 4.5-5.5 phosphate buffering solution in the concentration of 15-20 ml / g with cellulolytic enzyme in enzyme activity of 5-10 U / ml and enzyme concentration of 0.5-1 % to enzymolyze at 100-120 rpm and 50-60 DEG C for 1-2 hr, before decolorizing and vacuum drying to obtain functional diet fiber. The method is simple and reasonable, and can utilize the fiber resource in waste bamboo shoot coats effectively, improving soluble diet fiber content of bamboo shoot coat. Furthermore, the method can develop a high grade bamboo shoots product and improve economic benefit of the bamboo shoots processing industry, and can effectively solve a series of environmental pollution problems caused by putrefaction for stacking of bamboo shoot coats.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural and sideline product waste, and relates to a method for preparing functional dietary fiber by enzymolyzing waste bamboo shoot shells. Background technique [0002] Bamboo shoots not only have the effects of sweet and cold in nature, beneficial to water and Qi, clearing away heat and reducing phlegm, calming and calming the nerves, but also have the characteristics of low fat, low sugar and multiple fibers, and are known as "the first vegetarian product". Today, when advocating healthy eating, it is more and more popular among people. According to incomplete statistics, the output of bamboo shoots in my country reached 2.1 million tons in 2000, and it is estimated that by 2010, the output of bamboo shoots will reach 3.2 million tons, and the output of bamboo shoot products will also increase to 800,000 tons. However, in the bamboo shoot processing process, the added value of bamboo s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/308A23L33/22
Inventor 贾燕芳石伟勇余巍倪亮汤少红罗光恩何延蒋玉根
Owner ZHEJIANG UNIV
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