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Recombined foot-and-mouth disease virus polypeptide fragment and and uses thereof

A technology of foot-and-mouth disease virus and polypeptide fragments, which is applied in the field of recombinant foot-and-mouth disease virus polypeptide fragments, can solve the problems of not being able to fully form a three-dimensional three-dimensional structure, infection by research personnel, and discomfort for widespread promotion, and achieve the effects of preventing diffusion, convenient operation, and good effects

Inactive Publication Date: 2007-12-05
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the production process of the recombinant gene obtained by the technology disclosed in the No. 03100579 patent requires the participation of virus samples, and the acquisition of virus samples is complicated and easy to cause infection to the research personnel, resulting in high manufacturing costs and is not suitable for Widely popularized; in addition, the obtained recombinant gene has a simple spatial distribution and cannot fully form a three-dimensional structure, resulting in low antigenicity

Method used

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  • Recombined foot-and-mouth disease virus polypeptide fragment and and uses thereof

Examples

Experimental program
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Embodiment 1

[0048] The invention provides a polypeptide fragment of recombinant foot-and-mouth disease virus, which is used to make gene products without the risk of virus spread. The preparation method of the polypeptide fragment of recombinant foot-and-mouth disease virus is as follows:

[0049] Structural Scheme of Gene Synthesis of Active Peptide (HXT)

[0050] According to the amino acid sequence of the immunoactive peptide of the main antigenic site A of the foot-and-mouth disease virus, the antigenic activity was analyzed by DNASTAR software. The base sequence of the active peptide (HXT5) gene is designed by computer using the preferred codons of Escherichia coli, and the EcoRI restriction site is designed. The seven alternatives are as follows, wherein the italic part in the sequence is the flexible amino acid, and the underlined part is the core fragment of the foot-and-mouth disease virus gene.

[0051] Option One:

[0052] ISISEIKGVIVHKIETILF-K-VYNGSSKYGDTSTNNV RGD LQVLAQKAE...

Embodiment 2

[0081] In this example, the recombinant foot-and-mouth disease virus polypeptide fragments are used to produce related gene products for the quarantine of foot-and-mouth disease virus.

[0082] Traditional animal quarantine is to cultivate pathogens or measure antibodies, such as application of neutralization test, enzyme-linked immunosorbent assay (Enzyme-Linked Immunosorbnent Assay, referred to as ELISA), rapid dot immunobinding assay (referred to as DIBA), agar diffusion test and complement fixation test, etc. At present, some new inspection and quarantine technologies have been popularized and applied, such as PCR, Western blotting, recombinant antigens, monoclonal antibodies, and synthetic peptides, which have become important supplements to traditional inspection and quarantine technologies. However, these new inspection and quarantine technologies require expensive experiments. Equipment and test conditions. Therefore, conventional diagnostic methods are still widely u...

Embodiment 3

[0096] As another embodiment of the present invention, the recombinant foot-and-mouth disease virus polypeptide fragment of the present invention can be used to prepare biochips, gene chips or vaccines, wherein at least one probe of the biochip adopts the recombinant foot-and-mouth disease virus polypeptide fragment of the present invention; At least one probe of the chip adopts a DNA segment transformed from the recombinant foot-and-mouth disease virus polypeptide fragment of the present invention; the ligand of the kit or test strip adopts the recombinant foot-and-mouth disease virus polypeptide fragment of the present invention; Foot-and-mouth disease virus polypeptide fragment.

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Abstract

The present invention reveals one kind of recombinant foot-and-mouth disease virus polypeptide fragment and its gene products. The recombinant foot-and-mouth disease virus polypeptide fragment includes two or more single-chain oligonucleotide segments and one or more flexible amino acid segments. Each two of the single-chain oligonucleotide segments are connected through one flexible amino acid segment, and at least one of the single-chain oligonucleotide segments contains the kernel segment RGD of the foot-and-mouth disease virus gene.

Description

technical field [0001] The invention relates to a recombinant foot-and-mouth disease virus polypeptide fragment, in particular to a recombinant foot-and-mouth disease virus polypeptide fragment that can be widely used in the fields of immunoassay, vaccine and the like in a safe and effective manner. Background technique [0002] Foot-and-mouth disease is a zoonotic infectious disease that can infect all artiodactyls in livestock. Its pathogenic source is Apthovirus (Apthovirus) in the Picornavirus genus, including seven serotypes: O, A, C, SAT1, SAT2, SAT3 and Asian 1. Currently prevalent in the UK is the pan-Asian O type, which is highly toxic and manifests as acute fever in animals, followed by blisters in the mouth and hooves of the animals. Foot-and-mouth disease is very harmful to the development of animal husbandry. Pigs, cattle, sheep and other cloven-hoofed animals are infected with this disease, the production performance will decline, and severe cases will cause ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/42C07K14/09C12Q1/68G01N33/68A61K39/135
Inventor 林志雄田纯见陈茹罗长保朱道中鱼海琼罗琼宋长绪
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU