Composite type biological surfactant and its production method

A biosurfactant and active agent technology, applied in chemical instruments and methods, transportation and packaging, chemical/physical processes, etc., can solve the problem that microorganisms cannot grow well at the same time, so as to improve the microenvironment of composting, increase types, and promote composting. Effect of Contact Density and Intensity

Inactive Publication Date: 2007-12-12
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if a variety of microorganisms capable of producing biosurfactants are inoculated in the compost environment at the same time, the

Method used

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  • Composite type biological surfactant and its production method
  • Composite type biological surfactant and its production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: produce composite biosurfactant

[0020] The bacterial classification used in the present invention is Bacillus subtilis (numbering CCTCC Bacillus subtilis AB93108), and this bacterial classification is purchased from China Typical Culture Collection Center, and is inoculated once on the slant medium every 1 to 2 months, and bacterial classification is in 4 Store under ℃ conditions. Take the refrigerated strains and activate them at 30°C for 24 hours, and then use an inoculation loop to pick a ring of strains from the slant culture medium in the aseptic operating table and inoculate them into the seed culture solution, which was pre-heated in a high-pressure steam cooker at 115 Sterilize at ℃ for 30min. The inoculated seed culture solution was placed in a constant temperature incubator for cultivation at 30° C. and 200 rpm. The composition of the seed culture solution is: NaCl 5.0g, peptone 5.0g, beef powder 3.0g, distilled water 1000mL, and adjust the p...

Embodiment 2

[0024] Embodiment 2: produce composite biosurfactant

[0025] As in Example 1, take the strains refrigerated at 4°C and activate them at 37°C for 24 hours, then use an inoculation loop to pick a ring of strains from the slant culture medium in the aseptic operating table and inoculate them in the seed culture solution, and the seeds are cultivated The solution was sterilized in a high-pressure steam cooker at 115°C for 30 min in advance. The inoculated seed culture solution was placed in a constant temperature incubator for cultivation at 37° C. and 250 rpm. The composition of the seed culture solution is: NaCl 4.0g, peptone 4.0g, beef powder 2.5g, distilled water 1000mL, and adjust the pH to 7.2 with 1M NaOH.

[0026] Inoculate the seed culture solution cultivated for 24 hours into the fermentation medium at an inoculum size of 5% by volume fraction, and the fermentation solution is composed of: glucose 45g, NH 4 NO 3 7.3g, K 2 HPO 4 ·3H 2 O 14.3g, NaH 2 PO 4 ·H 2 O...

Embodiment 3

[0030] Composition and properties of the prepared biosurfactant of embodiment 1 and 2:

[0031] 1. Lipids content

[0032] Take 0.15g of biosurfactant, dissolve and extract lipids with 6.0mL chloroform / methanol (V:V=2:1) ​​mixed solution, then centrifuge at 7332×g for 10min, collect the supernatant, and the residue in the centrifuge tube Extraction was continued by the above method, repeated 3 times. The collected organic extract phase was rotatively evaporated at 46° C., weighed, and the mass percentage of lipid in the biosurfactant was determined to be 98.1±0.2%. Because this method utilizes the solubility of lipids in organic matter, the measured lipid content is pure lipids and their combinations with other substances (such as lipopeptides, lipoproteins or glycolipids, etc.) of the total mass percentage.

[0033] 2. Protein content

[0034] The protein content in the biosurfactant was determined by the Coomassie brilliant blue method, bovine serum albumin was used as a...

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Abstract

The invention relates to a kind of complex biological surface active agent and the preparation method. It is prepared with bacillus subtilis, and the comprised component and their weight proportion are as follows: fat 98.1+- 0.2%, protein 19.1+- 0.2%, sugar 0.7+-0.1%, the critical micelle concentration of said complex biological surface active agent are 4.2- 5.0 g/l and 1.25- 1.39 g/l respectively, and they reduce interfacial force of distilled water from 78 m/m to 38.4- 39.8 mN/m, and 40.3- 40.8 mN/m. The invention is characterized in that it enriches biological surface active agent species generated by bacillus subtilis, and make it possible for bacillus subtilis wide application in environmental pollution treatment.

Description

technical field [0001] The present invention relates to a new composite biosurfactant produced by Bacillus subtilis and a suitable extraction method for the biosurfactant. Background technique [0002] Biosurfactants have gradually been paid attention to because of their non-toxic or low-toxicity and biodegradability, and are gradually replacing "traditional surfactants" in industrial applications. However, problems such as the high cost of biosurfactants and the limited variety in industrial applications have become the bottlenecks for their wide adoption in industry. In the field of solid waste composting, biosurfactants can improve the microenvironment of composting, thereby promoting the decomposition of organic waste by microorganisms and improving the treatment efficiency of waste in composting. Garbage composting is a complex reaction system, and a variety of biosurfactants working together in the composting system will be more conducive to improving the composting m...

Claims

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Application Information

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IPC IPC(8): C12P21/00B01F17/30C05F9/04C09K23/30
CPCY02A40/20
Inventor 刘智峰曾光明陈耀宁傅海燕钟华刘小兰
Owner HUNAN UNIV
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