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Gas production enterobacteria and uses thereof

A technology of Enterobacter aerogenes and short rod, which is applied in the directions of bacteria, process efficiency improvement, fermentation, etc., can solve the problems such as inability to utilize organic matter, no discovery of Enterobacter aerogenes, unfavorable large-scale production and practical application, etc.

Inactive Publication Date: 2008-01-02
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these strains with iron reduction activity still have the following defects: 1) most strains can only survive in a strict anaerobic environment, which is not conducive to large-scale production and practical application; 2) the electron donor utilization spectrum of the strains is narrow, only It can use small molecular weight organic substances such as acetic acid and lactic acid as electron donors, but cannot use organic substances with large molecular weight such as glucose and sucrose, and has relatively high environmental requirements for practical applications.
Enterobacter aerogenes is a ubiquitous strain in nature, but so far no report of Enterobacter aerogenes with iron-reducing activity has been found

Method used

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  • Gas production enterobacteria and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 strain isolation

[0021] 1) Separation medium: Each liter of separation medium contains 1.0g glucose; 16g ferric citrate; 2.5g NaHCO 3 ;0.25g NH 4 Cl; 0.678g NaH 2 PO 4 2H 2 O; 0.1g KCl; vitamin solution 10.0mL; trace element solution 10.0mL; pH 5.5. Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, 10.0mg vitamin B6, 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin B12, 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; trace element solution contains 1.5g nitrilotriacetic acid, 3.0g MgSO per liter of water 4 ·7H 2 O, 0.5g MnSO 4 ·H 2 O, 1.0gNaCl, 0.1gFeSO 4 ·7H 2 O, 0.1g CoCl 2 ·6H 2 O, 0.1g CaCl 2 , 0.1gZnSO 4 ·7H 2 O, 0.01g CuSO 4 ·5H 2 O, 0.01g AlK(SO 4 ) 2 12H 2 O, 0.01gH 3 BO 3 , 0.01g Na 2 MoO 4 2H 2 O.

[0022] 2) Enrichment: Weigh 5g of ancient forest soil samples and inoculate them into 100mL of the above-mentioned liquid separation medium; fill the culture medium...

Embodiment 2

[0024] Example 2 Characterization

[0025] (1) Morphological characteristics of bacteria

[0026] Observed by a conventional bacterial electron microscope, the strain is short rod-shaped, with a size range of 1.0-1.7×0.6-0.8 μm. (see picture 1)

[0027] (2) Colony Morphological Characteristics

[0028] After 24 hours of aerobic culture on the beef extract peptone solid medium plate, the colony form is round, with neat edges; smooth surface, low convex surface; white, and the colony diameter is 2 ~ 3mm; in the solid separation medium described in Example 1 After 48 hours of anaerobic culture on the plate, the colony is round in shape with neat edges; the surface is smooth and highly convex; yellow, and the diameter of the colony is 2-3 mm.

[0029] (3) Physiological and biochemical characteristics

[0030] According to the BioLog microbial identification instrument, the utilization of 96 different carbon sources by this strain under aerobic conditions can be analyzed, and t...

Embodiment 3

[0037] Example 3 Iron reduction activity

[0038] The electron donor is citric acid and the electron acceptor is ferrihydrite

[0039] Medium (a): Add 2.0g citric acid, 10.0g ferrihydrite, 2.5g NaHCO to 1.0L deionized water 3 , 0.25g NH 4 Cl, 0.678g NaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin solution, 10.0mL trace element solution; wherein, vitamin solution and trace element solution formula are the same as in Example 1; adjust the pH of medium (a) to 5.5, and sterilize at 121°C for 20 Minutes, after cooling, fill with high-purity mixed gas (N 2 / CO 2 =80 / 20) 1 hour. Inoculate the bacteria from the slant of preserved Enterobacter aerogenes XM02 in beef extract peptone liquid medium, activate the bacteria at 30°C, 180 rpm shaker for 9 hours, so that the number of bacteria reaches the exponential growth phase, and inoculate with 10% A small amount of activated bacteria solution was inoculated in the above medium (a), and the anaerobic culture was static at 30°C, and a c...

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Abstract

The invention discloses an enterobacteria aerogenes and the application. The inventor screens Enterobacter aerogenes XM02 by a great deal of experimental researches. The bacterial has ferrum redactum active, facultative and electron donor, which can survive in the aerobic or anaerobic environment, wherein the electron donor in the anaerobic environment has the wide application, the electron donor which can electron donor comprises glycerin, citrate, dextrose, sucrose or the like, the ferric iron which can deacidize comprises szomolnokite, goethite, lepidocrocite, bloodstone, yellow copiapite or the like.

Description

technical field [0001] The invention relates to the technical field of screening new strains, in particular to an Enterobacter aerogenes and its application. Background technique [0002] The average content of iron in the earth's crust is 5.6%, which is the second most abundant metal in the earth's crust. Iron usually exists in the form of insoluble Fe(III) oxides in the natural environment. The reduction of iron is an enzymatic reaction mediated by specific microorganisms. Bacteria with iron reduction ability are the driving force for the reduction of iron oxides. Studies have shown that iron reduction not only affects the biogeochemical cycle of iron, but also participates in a variety of soil environmental processes, including: soil gleization process, migration of nutrients (such as P, K, Ca, Mg, etc.) and trace metal elements release, anaerobic degradation of organic pollutants, reductive detoxification of metals, and suppression of methane emissions, etc., have broad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00C22B3/18
CPCY02P10/20
Inventor 周顺桂李晓敏雷发懋李芳柏
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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