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Glucoside type flavone biological transformation and purification technique

A biotransformation, aglycone-type technology, applied in the biological field, can solve the problems of many by-products, anti-oxidative adverse effects, environmental pollution, etc., and achieve the effects of less by-products, no environmental pollution, and improved biological titer.

Inactive Publication Date: 2010-05-12
山东春天药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods often shield the main functional group of flavonoids - the phenolic hydroxyl group, during the reaction process, which has adverse effects on antioxidants.
Simultaneously, these methods have many by-products, which easily cause environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 5 kg of ginkgo flavonoids, add purified water to fully dissolve, then add purified water to 1000 kg, adjust the pH value of the solution to 5 with dilute hydrochloric acid, adjust the solution to 50°C, and accurately weigh α-amylase and cellulase 250 grams each; dissolve in a small amount of purified water, add to the solution, stir, and react for 15 hours, then accurately weigh 50 grams each of β-glucanase, pectinase, and xylanase, dissolve in a small amount of water, and add to the reaction solution , reacted for 12 hours. Adjust the pH value to 3.8 with dilute acid solution, adjust the temperature to 40°C, add 10 kg of naringinase, react for 20 hours, and the reaction is complete. Concentrate the reaction solution under reduced pressure to a certain level, centrifugal filter, absorb the supernatant on a DM-130 resin column, rinse with 2 times pure water, elute with 70% ethanol solution, concentrate the eluent under reduced pressure, control the temperature at 7...

Embodiment 2

[0028] Weigh 10 kg of rutin, add tap water to fully dissolve, then add water to 1000 kg, adjust the pH of the solution to 5.5 with dilute hydrochloric acid, adjust the solution to 55°C, and accurately weigh 100 grams each of α-amylase and cellulase Dissolve in a small amount of purified water, add to the solution, stir, react for 12 hours, then accurately weigh 50 grams each of β-glucanase, pectinase, and xylanase, dissolve in a small amount of water, add to the reaction solution, and react for 12 hours Hour. Adjust the pH value to 3.5 with dilute acid solution, adjust the temperature to 50° C., add 20 kg of naringinase, react for 24 hours, and the reaction is complete. Concentrate the reaction solution under reduced pressure to a certain level, centrifugally filter, absorb the supernatant on a D101 resin column, rinse with 2 times pure water, elute with 80% ethanol solution, concentrate the eluent under reduced pressure, control the temperature at 65°C, and dry the thick past...

Embodiment 3

[0030] Weigh 8 kg of baicalin, add tap water to fully dissolve, then add water to 1000 kg, adjust the pH of the solution to 6 with dilute hydrochloric acid, adjust the solution to 60°C, and accurately weigh 150 grams each of α-amylase and cellulase Dissolve in a small amount of purified water, add to the solution, stir, react for 24 hours, then accurately weigh 50 grams each of β-glucanase, pectinase, and xylanase, dissolve in a small amount of water, add to the reaction solution, and react for 12 Hours. Use dilute acid solution to adjust the pH value to 3.5, adjust the temperature to 50°C, add 20 kg of naringinase, react for 24 hours, and the reaction is complete. Concentrate the reaction solution under vacuum and decompression to a certain value, centrifugal filter, and put D101 resin on the clear liquid Column adsorption, 2 times of pure water washing, elution with 60% ethanol solution, concentration of eluent under reduced pressure, temperature control at 60°C, vacuum dryin...

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PUM

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Abstract

A process concerned with aglycon-anthoxanthin bio-conversion and purification is carried out by: dissolving anthoxanthin, regulating pH and temperature, adding enzyme A, B, C sequentially and acting aperiod respectively, concentrating, separating, adsorbing with macro-porous resin column and adsorbing, and concentrating and drying to obtain final product. The process has advantages such as moderate reaction conditions, less by-products and environment pollution, with higher value and without steric effect.

Description

(1) Technical field [0001] The invention relates to a biological technology, in particular to a method for the biotransformation and purification of aglycone flavonoids. (2) Background technology [0002] The metabolic pathways of flavonoids in animals are different. Only aglycon-type flavonoids can be directly absorbed into the blood of animals, while most glycoside-type flavonoids cannot enter the blood through the small intestine wall in the human body. Under the action of colonic microorganisms, some flavonoids can be hydrolyzed into aglycones before entering the blood. Therefore, the bioavailability of flavone glycosides in animals is much lower than that of aglycone flavones. Therefore, improving the configuration of flavonoids and increasing their absorption rate in blood is an important way to increase the bioavailability of flavonoid products. At present, there are mainly chemical and biological methods for the configuration transformation of flavone glycosides. M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 颜廷和
Owner 山东春天药业有限公司
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