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Preparation method of chitosan composite nerve conduit

A technology of nerve conduit and chitosan, which is applied in conduits, devices of human tubular structures, etc., can solve the problems of high energy consumption and low production efficiency, and achieve the effects of high tensile strength, growth inhibition, and regeneration promotion.

Inactive Publication Date: 2008-03-12
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the development of collagen nerve conduits is made by freeze-drying molding method, which consumes a lot of energy and has low production efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of chitosan composite nerve guide of the present invention

[0024] Preparation of the first step chitosan solution

[0025] Take fresh shrimp shells, remove the head, feet and tail, wash them with water, soak them in 5% HCl and 10% NaOH respectively to remove calcium and protein, wash until neutral, dry, and grind to obtain powdered chitin. Take powdered chitin and deacetylate it in 50% NaOH solution above 100°C for more than 24 hours, then neutralize, wash with water for injection until neutral, dehydrate with alcohol, and finally vacuum dry to obtain a shell with a degree of deacetylation of 85-95% glycans. The chitosan was dissolved in 2% (V / V) acetic acid to make the final chitosan solution concentration 1.5% (W / V).

[0026] Preparation of the second step collagen solution

[0027] Beef Achilles tendon is used as raw material, after degreasing and cleaning, freezing, slicing, crushing with a high-speed tissue grinder, eluting, and c...

Embodiment 2

[0031] Embodiment 2: the preparation of chitosan composite nerve guide of the present invention

[0032] The preparation of the first step chitosan solution and the preparation of the second step collagen solution are the same as example 1.

[0033] Preparation of the third step chitosan-collagen composite solution

[0034] The above collagen solution and chitosan solution were mixed according to the volume ratio of 6:4, and the solution was mixed uniformly by ultrasonic wave, and then centrifuged for 30 minutes with a high-speed centrifuge to defoam.

[0035] The preparation of the fourth step of molding the multi-layer composite nerve guide is the same as that of Example 1.

Embodiment 3

[0036]Embodiment 3: the preparation of chitosan composite nerve guide of the present invention

[0037] The preparation of the first step chitosan solution and the preparation of the second step collagen solution are the same as example 1.

[0038] Preparation of the third step chitosan-collagen composite solution

[0039] The above-mentioned collagen solution and chitosan solution were mixed according to the volume ratio of 7:3, and the solution was mixed evenly with ultrasonic waves, and then centrifuged with a high-speed centrifuge for 30 minutes to defoam.

[0040] The preparation of the fourth step of molding the multi-layer composite nerve guide is the same as that of Example 1.

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Abstract

The present invention relates to the technical field for the medical and biological material. In the recent ten years, researchers all over the world have proved that it is possible that the nerve frame can replace the antilogous transplantation in nerve defect repairing. But the chitosan tube of a single element has poor elastic property, which is easy to fracture; therefore the nerve stump is hard to be fixed in the tube. The present invention provides a preparation method for the chitosan nerve tube, which has great stretching intensity, long time maintenance in the body and capacity of biodegradation and absorption. In the present invention, the collagen protein and the acid solution of the chitosan are mixed into a composite according to a proper volume ratio. The composite liquid receives layers solidification on the model. The animal test and the clinical research discover that the present invention is capable of bridge joint between the nerve defect ends; the stretching intensity is strong and maintenance in the body is long; reproduction of the nerve is promoted. The technique of the present invention is simple with low cost, which can replace the traditional nerve joint method. The present invention has good application prospect in clinical treatment for the peripheral nerve defect.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a preparation method of a biodegradable chitosan composite nerve guide, which is especially suitable for use as a nerve bridge when treating peripheral nerve defects. Background technique [0002] The repair of peripheral nerve defects has always been a clinical problem. The excellent and good rate of traditional repair methods such as adventitial suture is only 50%. In the field of trauma surgery, nerve damage is repaired by nerve end-to-end suture. However, because the peripheral nerve is a mixed nerve, there is still a lack of fast and effective surgery. The scientific identification method to accurately distinguish the sensory nerve and motor nerve fibers at the nerve stump, even if the perineural suture is performed with fine microsurgical techniques, it is difficult to avoid the wrong butt joint of the sensory and motor nerve fibers after the end-to-end suture ...

Claims

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Application Information

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IPC IPC(8): A61L29/06A61F2/04
Inventor 侯春林顾其胜蒋丽霞
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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