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Novel acid tolerant lactobacillus sakei probio- 65 with the ability of growth suppression of pathogenic microorganisms and the anti-allergic effect

A kind of technology of Lactobacillus sack, pathogenic microorganism, applied in the field of new lactic acid bacteria

Active Publication Date: 2011-04-20
PROBIONIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the emergence of antibiotic-resistant bacteria and residual antibiotics in livestock products, current regulations in many countries limit the use of antibiotics in animal feed and emphasize organic feeding programs

Method used

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  • Novel acid tolerant lactobacillus sakei probio- 65 with the ability of growth suppression of pathogenic microorganisms and the anti-allergic effect
  • Novel acid tolerant lactobacillus sakei probio- 65 with the ability of growth suppression of pathogenic microorganisms and the anti-allergic effect
  • Novel acid tolerant lactobacillus sakei probio- 65 with the ability of growth suppression of pathogenic microorganisms and the anti-allergic effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Isolation of Lactobacillus strains

[0066] The homemade kimchi juice (fermented) was diluted, spread on a BCP solid medium (Eiken Chemical, Japan), and cultured at 37°C for 48 hours. After the bacteria appeared under the microscope, a total of 480 micrococcus and bacilli were selected and cultured on MRS medium (Difco) and stored at -80°C. Finally, a microorganism that inhibits the proliferation of Staphylococcus aureus KCTC1621, which is a factor in the deterioration of atopic diseases, was selected and named Lactobacillus sakei Probio-65 (Figure 6).

Embodiment 2

[0067] Example 2: Identification of the isolated Lactobacillus sakei Probio-65 strain

[0068] The Probio-65 strain isolated in Example 1 was cultured at 37°C in MRS medium (Difco). In order to identify the Probio-65 strain, the morphological and physiological characteristics of the Probio-65 strain were used in the literature: Yoon et al., Int. J. Syst. Bacteriol., 47, 904, 1997 described using the API32A and API CHL system (BioMerieux) Method to determine. The nucleotide sequence of the 16S rDNA gene was determined and analyzed using the method described in Yoon et al., Int. J. Syst. Bacteriol., 47,933, 1997.

[0069] The Probio-65 strain is a gram-positive bacteria. This strain can survive under both aerobic and anaerobic conditions, does not form spores, is immobile and is a bacillus type. The optimum temperature range for the growth of this strain is 30°C-37°C. The Probio-65 strain does not produce gas and indole, does not show solubility properties, and cannot reduce nitr...

Embodiment 3

[0073] Example 3: Evaluation of the inhibitory effect of isolated microorganisms on harmful microorganisms

[0074] The method described by Kuroiwa et al. (Kuroiwa et al., Journal of Infections, 64, 257, 1990) was used to evaluate the growth inhibitory activity of primary isolated microorganisms against harmful pathogenic microorganisms. In this antibiotic activity evaluation, ten microbial strains were used: Escherichia coli KCTC2441, Klebsiella pneumoniae KCTC2208, Staphylococcus aureus KCTC1621, Staphylococcus epidermidis KCTC1917, Shigella freundii KCTC2008, Salmonella gallisepticum, cloacae Bacillus KCTC2361, Salmonella typhimurium, Citrobacter freundii KCTC2006 and Methanosporium KCTC2591.

[0075] The harmful pathogenic microorganisms were cultured in nutrient broth (NB) at 37°C for 18 hours. 0.1 ml of each culture solution was spread on a nutrient agar (NA) plate and air-dried, and a cylinder with a diameter of 8 mm was placed on each plate. The primary isolated microorga...

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Abstract

Disclosed are a novel lactic acid bacterium, Lactobacillus sakei Probio-65, and the use thereof. The L. sakei Probio-65 strain has acid tolerance, bile acid tolerance and antibiotic resistance, inhibits the growth of harmful pathogenic microorganisms in the body and the intestine of animals, and has immunoenhancing activity. In particular, the novel strain inhibits the growth of Staphylococcus aureus, which is known to be a factor aggravating atopic dermatitis. Thus, the novel strain is useful for preventing or treating atopic dermatitis and allergy-related disorders. Also, the novel strain stabilizes intestinal microflora by inhibiting the abnormal proliferation of harmful microorganisms in the intestine. The L. sakei Probio-65 strain or a culture thereof is useful in pharmaceutical, feed, food, and cosmetic compositions.

Description

Technical field [0001] The present invention relates to a new lactic acid bacteria, namely Lactobacillus saccharomyces probiotic-65 and uses thereof. [0002] More particularly, the present invention relates to acid-resistant, bile acid-resistant and antibiotic-resistant, and effective inhibition of the growth of harmful pathogenic microorganisms and anti-allergic activity of Lactobacillus Sakei Probio-65 (Lactobacillus sakei Probio-65) and containing this Microorganism or its cultured medicine, feed, food and cosmetic composition. Background technique [0003] Atopic dermatitis is an inflammatory skin disease characterized by severe itching and eczema skin lesions and mainly affects infants and children. Especially in chronic cases, atopic dermatitis often recurs in winter. The atopic dermatitis thus occurring often progresses to bronchial asthma or allergic rhinitis, and it sometimes recurs in adults. The itching, which makes atopic dermatitis patients very painful, can lead t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61Q19/00C12R1/225A61K8/99A23L1/30A61K35/74A23K1/00C12N1/20A61K35/747
CPCC12R1/225A61Q17/005A23L1/3014A61K8/99A23K1/009A61Q19/00A61K35/747A23K10/18A23L33/135A61K8/9728C12R2001/225C12N1/205G09B23/24G09B5/02F21K9/00
Inventor 朴勇河李仁善金鸿翼姜国熙
Owner PROBIONIC CO LTD
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