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Method for vapour-exploding stalk enzymolysis coupling ferment for hydrogen production by using immobilized cell

A technology of immobilized cells and steam-exploded straw, which is applied in the field of fermentation technology, can solve the problems that cellulase activity is difficult to develop normally and fermentation efficiency is difficult to improve, and achieves high resistance, easy separation, and the effect of releasing product inhibition

Inactive Publication Date: 2008-05-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the optimum temperature for enzymatic hydrolysis of cellulose is 50°C, when C. The optimum temperature difference is 15°C, which makes it difficult for the activity of cellulase to develop normally, and it is difficult to improve the fermentation efficiency

Method used

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  • Method for vapour-exploding stalk enzymolysis coupling ferment for hydrogen production by using immobilized cell
  • Method for vapour-exploding stalk enzymolysis coupling ferment for hydrogen production by using immobilized cell

Examples

Experimental program
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Embodiment 1

[0031] Embodiment 1. Using the method provided by the present invention to use steam-exploded corn stalks as raw material to carry out enzymatic hydrolysis coupled fermentation hydrogen production,

[0032] Specific steps are as follows:

[0033] 1. Put the raw material of steam-exploded corn stalks in an enzymolysis tank, add 25 IU / g-substrate of cellulase and tap water with pH 4.8 adjusted with sulfuric acid, the water contains urea 2g / l, KH 2 PO 4 0.51g / l, MgSO 4 0.31g / l, the solid-liquid ratio of steam-exploded straw and aqueous solution is 1:8 (w / v), enzymatically hydrolyzed at 50°C;

[0034] 2. Preparation of Clostridium butyricum (C.butylicum AS1.209) seed culture:

[0035] Each liter of seed medium includes the following components: glucose 20g, yeast extract 3g, KH 2 PO 4 0.2g, K 2 HPO 4 1.6g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, CaCl 2 0.01g, Na 2 S·9H 2 O 0.25g, NaMoO 4 2H 2 O 0.01g, NaHCO 3 0.2g and (NH 4 ) 2 SO 4 3.0g; Clostridium butyricum was...

Embodiment 2

[0039] Example 2. Using the method provided by the present invention to use steam-exploded wheat straw as raw material to carry out enzymatic hydrolysis coupled fermentation hydrogen production

[0040] Specific steps are as follows:

[0041] 1. Put the raw material of steam-exploded wheat straw in an enzymolysis tank, add 25IU / g-substrate of cellulase and tap water with pH 4.8 adjusted with sulfuric acid, the water contains 2g / l of yeast extract, KH 2 PO 4 0.51g / l, MgSO 4 0.31g / l, the solid-liquid ratio of steam-exploded straw and aqueous solution is 1:10 (w / v), enzymatically hydrolyzed at 50°C;

[0042] Step 2 is the same as embodiment 1;

[0043] 3. Mix the seed liquid of Clostridium butyricum (C. butyricum AS1.209) in the logarithmic growth phase with a sterile 3% sodium alginate solution, and the volume ratio of the seed liquid to the sodium alginate solution is 1:4. Use a sterile syringe to draw the mixture, slowly drop the mixture into sterile 0.05mol / l CaCl 2 so...

Embodiment 3

[0046] Example 3. Using the method provided by the present invention to use steam-exploded rice straw as raw material to carry out enzymatic hydrolysis coupled fermentation hydrogen production, the specific steps are as follows:

[0047] 1. Put the raw material of steam-exploded rice straw in an enzymolysis tank, add 25 IU / g-substrate of cellulase and tap water with pH 4.8 adjusted with sulfuric acid, the water contains urea 2g / l, KH 2 PO 4 0.51g / l, MgSO 4 0.31h / l, the solid-liquid ratio of the steam-exploded straw and the aqueous solution is 1:12 (w / v), and the enzyme is hydrolyzed at 50°C.

[0048] Step 2 is the same as in Example 1.

[0049] 3. Mix the seed liquid of Clostridium butyricum (C. butyricum AS1.209) in the logarithmic growth phase with a sterile 4% sodium alginate solution, and the volume ratio of the seed liquid to the sodium alginate solution is 1:5. Use a sterile syringe to draw the mixture, slowly drop the mixture into sterile 0.05mol / l CaCl 2 solution...

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Abstract

The invention relates to a hydrogen-produce method, which utilizes the immobilized cell to produce hydrogen though uncoupled fermentation of steam explosion straw enzyme. The invention puts the steam explosion straw and clostridium butyricum microorganism into different reactors; therefore, the two can react simultaneously in the respective best temperature. The clostridium butyricum immobilized cell is utilized to make the thalli stay in fermenting phase and the zymotic fluid is continuously pumped into the zymohydrolysis phase; therefore, the sugar produced by zymohydrolysis is watered down and then moves back to the fermentation cylinder circularly. The invention has the advantages of keeping the microorganism in high active condition and increasing the reaction efficiency and the productive rate of hydrogen.

Description

technical field [0001] The invention belongs to the technical field of fermentation technology, and mainly relates to a hydrogen production method for enzymatic hydrolysis of steam-exploded straw coupled with fermentation by using immobilized cells Background technique [0002] Hydrogen is a clean and efficient energy source and a good substitute for fossil fuels. Conventional hydrogen production methods, such as electrolysis of water and thermal cracking of fossil raw materials (Adnan M.Int J Hydrogen Energy, 2001: 26: 29-27; Ayhan D. Energy Sources, 2002: 24: 59-68.), exist However, it has the disadvantages of high energy consumption, complex product components, and high separation costs, and cannot fundamentally solve energy and environmental problems. Using renewable biomass resources as raw materials and relying on microbial fermentation to produce hydrogen has increasingly become a research direction and focus. [0003] Straw is a kind of renewable resource with abun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N11/04C12R1/145
Inventor 陈洪章李冬敏
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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