Method and device for transplantation of femtosecond laser nucleus

Abstract

The invention relates to a method and equipment for femtosecond laser somatic cell nuclear transplantation, in particular to a micro-nano control method which adopts a light knife effect induced by femtosecond laser two-photon and optical tweezers gradient force induced by high photon density. A pump laser is started-up; the laser produced by the pump laser is introduced into a resonator of a femtosecond laser; when the oscillation is done, the energy of the ultrashort femtosecond laser pulse is amplified through a regenerative amplifier, and the pulse number and the repeated frequency of laser output are chosen. The emitted femtosecond laser is divided into two beams of light, one of which used for realizing the femtosecond optical tweezers, and the other of which is used for realizing the function of the femtosecond optical tweezers through a half-permeable and semi-reflecting mirror, and then the two beams of light are coupled with an optical path through another half-permeable andsemi-reflecting mirror to realize the amplification of a spot light area; then the light beams is focused on a target cell, and the harmless transplantation operation of the somatic cell nuclear is done. A switch for controlling a light valve is arranged in a software control system, which can not only realize the operation of the laser spot on the target cell, but also adjust the scanning speed and change the exposure time to meet different requirements and enhance the success rate of the somatic cell nuclear transplantation.

Description

technical field [0001] The invention relates to a biological cell technology, which is a micro-nano manipulation method using the photoknife effect induced by femtosecond laser two-photon and the gradient force of optical tweezers induced by high photon density. The study of mass interactions. Background technique [0002] Cloning technology has shown broad application prospects, such as cultivating excellent livestock breeds and producing experimental animals, producing transgenic animals, and producing human embryonic stem cells for cell and tissue replacement therapy, etc. Although cloning technology has broad application prospects, it is still far from industrialization, and the success rate of cloning animals in practice is still very low. [0003] Cloning methods mainly include embryo segmentation and nuclear transfer. The so-called nuclear transfer technology is to transfer the nucleus of the donor cell into the denucleated oocyte, so that the latter can be activate...

AI Technical Summary

Problems solved by technology

However, traditional cell nuclear transfer methods, such as micromanipulation based on micropipettes and electrofusion transplantation, inevitably cause defects such as large-area cell membrane damage, loss of cytoplasm, and incomplete removal of nuclei, which affect the success rate of nuclear transfer.

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