Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same

A malodorous gas and microorganism technology, applied in the field of microorganisms, can solve difficult problems such as NH3 removal efficiency and difficulty, and achieve the effects of simple composition and culture conditions, easy use and simple operation

Inactive Publication Date: 2008-06-18
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to differences in the suitable growth factors (such as pH, nutritional requirements) of different microorganisms, it is difficult to remove H 2 S and except NH

Method used

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  • Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same
  • Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Study on the growth temperature of Thiobacillus thiooxidans CCW-Y2 strain

[0036] Use SOB medium, pH=3.0, culture in the temperature range of 15-55°C, and test on a shaker with temperature control ability in a temperature control room. The amount of sulfur oxidized to form sulfate ions and the pH change in the culture solution were used to indirectly calculate the oxidation of elemental sulfur and the growth state of bacteria. The growth results at 5 days of culture are shown in Table 1.

[0037] Table 1 Growth of CCW-Y2 strain at different temperatures

[0038] Culture temperature (℃)

[0039] From the data in Table 1, it can be seen that the optimal temperature for growth of the CCW-Y2 strain is 28-30°C, which is a mesophilic bacteria. It can still grow below 15°C, but hardly grows above 55°C.

Embodiment 2

[0040] Example 2 Study on the growth pH of Thiobacillus thiooxidans CCW-Y2 strain

[0041] Use SOB medium, temperature 30°C, and cultivate in the range of pH=0.5-5.5, and the experiment is carried out on a shaker with temperature control ability in a temperature control room. The amount of sulfur oxidized to form sulfate ions and the pH change in the culture solution were used to indirectly calculate elemental sulfur oxidation and the growth state of bacteria. The growth results at 5 days of culture are shown in Table 2.

[0042] Table 2 Growth of CCW-Y2 strain at different pH

[0043] PH of culture medium

[0044] The data in Table 2 shows that the CCW-Y2 strain can grow at a pH of 0.5 to 5.5, and the optimal pH for growth is 2.0 to 3.0, and it is an acidophilic bacteria.

Embodiment 3

[0045] Example 3 Study on ammonium sulfate tolerance of Thiobacillus acidophilus thiooxidans CCW-Y2 strain

[0046] A series of SOB culture media with different contents of ammonium sulfate were used for cultivation at a temperature of 30°C and pH=2.5. The experiment was carried out on a shaker with temperature control ability in a temperature control room. The specific sulfur oxidation rate (the amount of sulfur oxidation per unit mass of cell DCW per unit time) was used to investigate elemental sulfur oxidation and the growth state of bacteria. The growth results at 5 days of culture are shown in Table 3.

[0047] Table 3 Growth of CCW-Y2 strain under different ammonium sulfate concentrations

[0048]

[0049] It can be seen from the data in Table 3 that when the concentration of ammonium sulfate increases from 0.5 g / L to 10 g / L, the specific sulfur oxidation rate increases accordingly. But when the concentration of ammonium sulfate increased from 15g / L to 8...

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Abstract

A microorganism which can remove the malodorous gas of NH3 and H2S synchronously and a preparation method thereof relate to the microorganism technical field and in particular to an efficient deodorization microorganism of the mixed malodorous gas in a sewage collecting and disposing establishment and the preparation method thereof. An acidithiobacillus thiooxidans CCW-Y2 strain provided by the present invention is conserved in the General Microorganism Center of China Committee for Culture Collections of Microorganisms, and the conversing serial number CGMCC No. is 2158. The strain is ammonium sulfate tolerant type and is obtained by sieving and separating from the activated sludge of the living sewage disposal factory. The culture conditions of the acidithiobacillus thiooxidans CCW-Y2 strain of the present invention are as follows: firstly, SOB culture medium is adopted, and the components of the culture medium are the dipotassium hydrogen phosphate of 2g/L, the potassium dihydrogen phosphate of 2g/L, the magnesium chloride (MgCl2/7H2O) of 0.2g/L, the ammonium sulfate of 10g/L, the ferrous sulfate (FeSO4/7H2O) of 0.01g/L and the powdered sulfur of 10g/L; secondly, the culture condition is the temperature of 28 DEG C to 32 DEG C and 180 circles per minute; thirdly, a carrier is water, and dilute sulfuric acid (or hydrochloric acid) is added for adjusting pH value to be 2.0 to 3.0. The acidithiobacillus thiooxidans CCW-Y2 strain of the present invention can be directly inoculated into a waste gas disposing establishment (such as a biological filtration tower) and can synchronously remove the mixed malodorous gas of the H2S and the NH3 efficiently, which simplifies the operation condition of the waste gas disposing establishment and ensures that the operation is more simple.

Description

Technical field [0001] The present invention provides a simultaneous and efficient removal of ammonia (NH 3 ) And hydrogen sulfide (H 2 S) Malodorous gas microorganisms and preparation methods thereof, which relate to the field of microbiological technology, especially related to high-efficiency deodorizing microorganisms for mixing malodorous gases in sewage collection and treatment facilities and preparation methods thereof. Background technique [0002] With the development of the economy and the improvement of people's living standards, stench has attracted more and more attention as a major environmental hazard. NH 3 And H 2 S is the most common malodorous substance, and is the main exhaust gas component widely present in industrial production processes (such as sewage treatment plants, manure treatment plants, food processing plants, leather industry, animal husbandry, and composting plants, etc.). It is not only toxic, but also corrosive. It is an irritating and odorous su...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/52B01D53/58B01D53/84
CPCY02A50/20
Inventor 叶芬霞
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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