Biological activity bone renovation material with bone-inducing factor control-release function and production method thereof

A bioactive, osteoinductive technology, applied in medical science, prosthesis, etc., can solve the problems of difficult treatment effect, uneven introduction, application limitations, etc., achieve good osteoinductive properties, achieve uniform distribution, and prolong bone release. The effect of the time of induction factor

Inactive Publication Date: 2008-07-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the biological activity of osteoinductive factors can be retained to the greatest extent, but there are problems of uneven introduction and fast release, and it will also have an adverse effect on the porosity and pore permeability of the scaffold material, so that The therapeutic effect is difficult to achieve, and the application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Preparation of BMP-2 chitosan controlled-release microspheres

[0030] Weigh 250mg of chitosan, dissolve it in 10mL, 20mL / L acetic acid solution, and make a solution with a concentration of 25g / L; after it is completely dissolved, add 1mg of bone morphogenetic protein-2; after stirring evenly, add dropwise In 100mL of liquid paraffin containing 25mL / L Span 80, emulsify for 2 hours under stirring; then add 40mL of sodium tripolyphosphate solution with a concentration of 50g / L dropwise, continue stirring for 4 hours and then precipitate; Wash with ether and isopropanol three times alternately, then rinse with deionized water, and freeze-dry to obtain BMP chitosan controlled-release microspheres. The results of in vitro testing show that the average particle size of the microspheres is 39.0 μm, and the encapsulation efficiency of BMP-2 reaches over 90%.

Embodiment 2

[0031] Embodiment 2: Preparation of BMP-2 active peptide chitosan controlled release microspheres

[0032] Take by weighing 250mg chitosan, be dissolved in the acetic acid solution of 10mL, 20mL / L, make the solution that concentration is 25g / L; After it dissolves completely, add 5mg bone morphogenetic protein-2 active peptide; After stirring evenly, Add dropwise to 100mL of liquid paraffin containing 25mL / L Span 80, and emulsify for 2 hours under stirring; then add dropwise 40mL of sodium tripolyphosphate solution with a concentration of 50g / L, and continue stirring for 4 hours before precipitation; Wash with petroleum ether and isopropanol three times alternately, rinse with deionized water, and freeze-dry to obtain the BMP-2 active peptide chitosan controlled-release microspheres. The results of in vitro testing show that the average particle size of the microspheres is 33.9 μm, and the encapsulation efficiency of the BMP-2 active peptide is over 80%.

Embodiment 3

[0033] Embodiment 3: Preparation of bioactive bone repair material comprising BMP-2 controlled-release microspheres

[0034] Weigh the molecular weight as 3.0×10 5 0.5g of polylactic acid was dissolved in 10mL 1,4-dioxane to make a solution with a concentration of 50g / L; after it was completely dissolved, 0.5g of nHAC dry powder was added, and 0.25g of Example 1 was added to prepare BMP-2 chitosan controlled-release microspheres; the mixture was fully stirred and mixed evenly at room temperature, then poured into polytetrafluoroethylene molds, and placed in a -20°C refrigerator for 24 hours; the frozen and formed materials were transferred to Freeze-dry in a lyophilizer for 72 hours to remove the 1,4-dioxane solvent, and then put the material into a vacuum drying oven with a temperature of 40°C and a vacuum of -0.1MPa for 48 hours to obtain BMP-containing -2 nano-hydroxyapatite / collagen / polylactic acid / microencapsulated chitosan bioactive bone repair material.

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Abstract

The invention discloses a nano bone repair material with biological activity and a preparation method thereof. The nano bone repair material with biological activity has controlled release effectiveness for bone inductive factors and belongs to the technical field of biomedical materials. The bone repair material with biological activity consists of nano-hydroxyapatite/ collagen composite nHAC, biodegradable polymer material polylactic acid PLA and chitosan controlled release microspheres for treating effective quantity of bone inductive factors. By adopting the method described in the patent claim 1 of physically mixing three materials and combining the three materials by supersonic dispersion technology, the bone repair material with biological activity is compounded and prepared. The invention is a novel bone which has excellent biocompatibility and biodegradability and better bone inductive property than bone inductive property reported in technical literature reports of the prior art, and is very hopeful to be widely used in clinic as the repair material of bone defects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials. More precisely, the present invention relates to a nano bioactive bone repair material with the function of controlling the release of osteoinductive factors for bone tissue defect repair and a preparation method thereof. Background technique [0002] The repair and reconstruction of human tissue damage has always been a difficult problem that modern medicine strives to solve. Bone tissue is the largest organ in the human body and is also the most prone to defects. Every year, millions of patients with bone tissue damage need surgical treatment. However, so far, the treatment methods used for bone defect repair and reconstruction have defects in varying degrees. Autologous bone transplantation is currently the most clinically used method, but it is a means of "healing injuries with injuries" at the cost of sacrificing healthy bone tissue, which will cause new defects in the bone do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/54
Inventor 冯庆玲牛旭锋崔福斋
Owner TSINGHUA UNIV
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