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Seed culture medium produced with lincomycin ferment optimized with response surface method

A culture medium, the technology of Streptomyces lincous, applied in the field of microbial fermentation, can solve the problems of prolonging the cultivation time, seed aging, long cultivation period, etc.

Inactive Publication Date: 2008-07-16
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the process of fermenting and producing lincomycin with Streptomyces lincoeum is very complicated, and there are the following defects in seed cultivation: the ratio of medium components is unreasonable, resulting in a small growth and reproduction rate of Streptomyces lincoebe, and a long culture period; It is complicated, and the method of intermediate feeding is often used to prolong the culture time to increase the bacterial concentration (PMV); when transplanting, the PMV is small, and the seeds are already aging, which seriously affects the normal progress of the lincomycin fermentation process, and the production efficiency is low

Method used

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  • Seed culture medium produced with lincomycin ferment optimized with response surface method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1 two-level factorial design (2Level Factorial Design)

[0080] In this example, significant influencing factors were found from the influencing factors of the culture medium, and a two-level factorial design was adopted. The experimental design, data analysis and response value optimization of this study were carried out using Design Expert 7.0 software.

[0081] The designed initial medium formula is (g / L): starch 14, glucose 28, soybean meal 20, corn steep liquor 26, sodium chloride 0.7, sodium nitrate 0.85, calcium carbonate 7, ammonium nitrate 2, ammonium sulfate 2.3, phosphoric acid Potassium dihydrogen 0.045.

[0082] According to previous experiments, starch, glucose, soybean cake powder, ammonium nitrate and ammonium sulfate are the key factors in the initial seed medium. Therefore, starch, glucose, soybean meal, ammonium nitrate and ammonium sulfate were selected as influencing factors for two-level factor design, and the contents of other compone...

Embodiment 2

[0093] Embodiment 2 Response surface design (Response surface design)

[0094] Response surface design was carried out with glucose and ammonium sulfate as significant influencing factors, and the content (g / L) of other factors in each design group was the same, namely, starch 18.7, soybean meal powder 22.3, ammonium nitrate 1.33, corn steep liquor 26, calcium carbonate 7. Sodium chloride 0.7, potassium dihydrogen phosphate 0.045, sodium nitrate 0.85. The experimental design and analysis results are shown in Table 6-Table 10, respectively.

[0095] Table 6 Setting of variable high and low level values

[0096]

variable

Numbering

Coded levels

-1

1

unit

glucose

ammonium sulfate

x 1

x 2

0.35

0.01

1.25

0.1

g / 30ml

g / 30ml

[0097] Table 7 Response surface design and experimental results

[0098]

run

factor

response

X 1

...

Embodiment 3

[0109] The preparation of embodiment 3 medium

[0110] The formulation of the culture medium is shown in Table 10.

[0111] Table 10

[0112] Medium 2

[0113] Method for preparing medium: Accurately weigh each component, mix each component, add appropriate amount of distilled water to dissolve, and constant volume, so that each component in the medium meets the ratio in the table.

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Abstract

The invention pertains to the field of microbial fermentation, which discloses a streptomyces lincolnensis seed culture medium; the culture medium comprises a carbon source, a nitrogen source, a phosphorus source and an inorganic salt; the culture medium further comprises the following necessary components: starch, glucose, soybean cake powder, ammonium nitrate and ammonium sulfate; the invention also discloses the optimized mixing ratio of all the necessary components. The culture medium of the invention has reasonable mixing ratio; the usage of the culture medium for culture can have high growth and reproduction rate of the streptomyces lincolnensis, short culture period and high bacteria concentration, thereby effectively avoiding the aging of the seeds and effectively improving the production efficiency of the follow-up lincomycin fermentation process.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and more specifically, the invention relates to an optimized seed medium for lincomycin fermentation production. Background technique [0002] Lincomycin (Lincomycin) is a useful pharmaceutical intermediate used in the preparation of antibiotics, which is usually produced by Streptomyces (such as Streptomyces lincomycin). [0003] At present, the process of fermenting and producing lincomycin with Streptomyces lincoeum is very complicated, and there are the following defects in seed cultivation: the ratio of medium components is unreasonable, resulting in a small growth and reproduction rate of Streptomyces lincoebe, and a long culture period; It is complicated, and the method of intermediate feeding is often used to prolong the culture time to increase the bacterial concentration (PMV); the PMV is small when transplanting, and the seeds are already aging, which seriously affects the normal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/565
Inventor 储炬李啸张嗣良庄英萍杭海峰王永红郭美锦夏建业
Owner EAST CHINA UNIV OF SCI & TECH
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