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Method for producing fumaric acid fully by using ligno-cellulose

A technology of lignocellulose and fumaric acid, applied in the field of bioengineering, can solve the problems of poor metabolic efficiency, difficult fermentation of mixed sugar, low conversion rate of xylose, etc., and achieve the effects of reducing production costs, avoiding low efficiency, and wide sources

Inactive Publication Date: 2008-07-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the research on how to effectively use lignocellulose by microorganisms mainly focuses on the aspect of using the mixed sugar solution obtained from lignocellulose degradation for fermentation to produce ethanol. However, due to the strong inhibitory effect of glucose on ethanol fermentation and the difficulties in the use of mixed sugars in ethanol fermentation, the development and improvement of production methods for this type of product have yet to be resolved.
Other studies are also limited to such aspects as separating and purifying the degraded sugar solution, and then using pure xylose to convert xylitol into xylitol. However, the low conversion rate and poor metabolic efficiency of xylose are one of the problems to solve the effective utilization of lignocellulose. The key issue, the complete utilization of lignocellulose still needs further improvement in the production method

Method used

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  • Method for producing fumaric acid fully by using ligno-cellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0034] This example illustrates the steps of degrading lignocellulose in a two-step method to obtain two kinds of sugar liquids.

[0035] (1) Lignocellulosic raw material is impurity-removed, and it is pulverized to 50 orders of particle diameter for subsequent use;

[0036] (2) Soak with 1% dilute acid and filter overnight;

[0037] (3) adding 3% dilute sulfuric acid, and treating at 200° C. for 120 minutes;

[0038] (4) After filtering, the filtrate is collected to obtain a hemicellulose degradation liquid, and the hemicellulose conversion rate is 70% to 90%. After Ca(OH) 2 adjusting the pH to 10, and then using an acid solution to adjust the pH to 7, filtering calcium sulfate precipitates and insoluble matter, and obtaining a hemicellulose degradation solution mainly containing xylose for seed cultivation;

[0039] (5) Treat the above remaining solid residue at 250°C with 3% sulfuric acid for 2 to 5 minutes, convert about 50% to 80% of the remaining cellulose into glucose, ...

Embodiment 2

[0044] This example illustrates the steps of fully utilizing lignocellulose degradation liquid to produce fumaric acid.

[0045] Bacteria: mold (spore suspension, the concentration is 10 6 each / mL)

[0046] (1) Prepare culture medium

[0047] a. Seed medium:

[0048] The xylose degradation solution obtained by degrading hemicellulose in Example 1 is concentrated to obtain a xylose solution with a concentration of 29.5g / L, adding the necessary nutrients for mold seed cultivation: (NH 4 ) 2 SO 4 2.5g / L, yeast extract 2.4g / L, corn steep liquor 2mL / L, KH 2 PO 4 0.4g / L, MgSO 4 ·7H 2 O 0.4g / L, ZnSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.01g / L, agar 2g / L, add H 2 SO 4 Adjust the pH to 4.5.

[0049] b. Fermentation acid production medium:

[0050] Evaporate, concentrate and detoxify the cellulose degradation solution in Example 1 and properly concentrate or dilute to obtain a glucose solution with a concentration of 80g / L, and add the necessary nutrients for acid producti...

Embodiment 3

[0060] The method of this embodiment is the same as that of embodiment 2, but the conditions are changed.

[0061] Strain: Rhizopus (spore suspension, concentration is 10 6 each / mL)

[0062] (1) Prepare culture medium

[0063] a. Seed medium

[0064] Dilute the xylose degradation solution obtained by degrading hemicellulose in Example 1 to obtain a xylose solution with a concentration of 10g / L, add the necessary nutrients for mold seed cultivation: (NH 4 ) 2 SO 4 1.5g / L, KH 2 PO 4 0.1g / L, MgSO 4 ·7H 2 O 0.1g / L, ZnSO 4 ·7H 2 O 0.01g / L, FeSO 4 ·7H 2 O 0.01g / L, add HCl to adjust the pH to 4.

[0065] b. Fermentation acid production medium:

[0066] Evaporate, concentrate and detoxify the cellulose degradation liquid in Example 1, then concentrate or dilute it appropriately to obtain a glucose solution with a concentration of 100g / L, and add the necessary nutrients for acid production by mold fermentation: KH 2 PO 4 0.1g / L, MgSO 4 ·7H 2 O 0.1g / L, ZnSO4 7H 2 O 0....

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Abstract

The invention discloses a preparation method for a fumaric acid fully using lignocellulose resources to ferment, and a two-step method to realize the lignocellulose degradation; the first step, proper nutrient substance is added into the hemicellulose degradation solution mainly containing xylose, to culture the seeds for producing fumaric acid mildew for 18 to 42 hours; the second step, proper nutrient substance is added into the cellulose degradation solution mainly containing glucose, to be used as a culture medium for fermentation and acid producing for inoculation, the seed liquid is inoculated and cultured for 60 to 96 hours to get high-yield fumaric acid; the yield is 28 to 56g / L, and the percent conversion is 41% to 52%. The preparation method for the fumaric acid makes full use of the material of the lignocellulose type materials, so the source is more extensive; the preparation method for the fumaric acid effectively reduces the production cost without separation and purification, and has an excellent industrial potential application.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for producing organic acids by microbial fermentation using lignocellulose resources, and in particular to a method for fully utilizing lignocellulose in microbial fermentation to produce fumaric acid. Background technique [0002] Fumaric acid is an important organic basic chemical raw material and fine chemical product, mainly used in the synthesis of unsaturated polyester resin, etc., widely used in coatings, resins, medicine, plasticizers and other fields, and can be It is used as an additive in the food and feed industry and in the pharmaceutical field. At the same time, as an important four-carbon platform compound, fumaric acid can also produce L-aspartic acid, malic acid, succinic acid and other four-carbon compounds through enzymatic conversion and other processes. The current mainstream technology for fumaric acid production is maleic anhydride isomeriz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/645
Inventor 黄和李霜高振刘宁何皓
Owner NANJING UNIV OF TECH