Time-domain fluorescent diffusion chromatography system facing to small animal molecule image-forming

Abstract

The invention relates to the small animal molecule imaging field, in particular to a time domain fluorescence diffusion chromatographic system facing the small molecule imaging field. In order to provide a method and device capable of obtaining high quantification precision, the three-dimensional chromatography of spatial resolution, stable double parameters and a polycomponent imaging capacity, the invention adopts the following technical proposal: the invention comprises two picosecond semiconductor lasers and fluorescent probes for providing laser with required super-short wave length, a 2:1 optical switch which is used to select the two paths of laser emitted by the two picosecond semiconductor lasers, a variable attenuator which is used to attenuate the strength of the laser emitted by the 2:1 optical switch to a required measuring range, an optical switch which is used to convert the light source with selected wave length emitted by the variable attenuator to a plurality of light source points, an incident optical fiber which is used to project the laser emitted by the light source points to an object body, and a receiving optical fiber which is used to receive the laser reflected by the object body or passing through the object body. The time domain fluorescence diffusion chromatographic system is mainly applicable to the time domain fluorescence diffusion chromatography during molecule imaging.

Description

technical field [0001] The invention relates to the field of molecular imaging of small animals, in particular to a time-domain fluorescence diffusion tomography system for molecular imaging of small animals. technical background [0002] With the completion of human genome sequencing and the arrival of the post-gene era, the pathogenesis and early biological characteristics of diseases are analyzed from the interaction relationship between nucleic acid-protein and protein-protein molecules, and then the early detection, early warning, and early detection of diseases can be effectively carried out. Diagnosis and efficacy evaluation have become a top priority in health monitoring and life science research. Driven by this, the real-time acquisition and quantification of physiological and pathological information of living organisms at the molecular level has become a hotspot in the research of medical imaging technology. Technically, molecular imaging can be based on the exis...

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Problems solved by technology

However, the steady-state mode can only provide fluorescence emission rate images. Although the frequency-domain mode can reconstruct the emission rate and lifetime parameters at the same time, the "cross-contamination" is serious; and due to the limitation of spatial sampling, the images of the steady-state and frequency-domain FMT techniques will deteriorate. effect

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