Alginate capsule immobilized with cell and preparation method thereof

An alginate and capsule technology, applied in directions such as being fixed on/in an organic carrier, can solve the problems of high preparation cost, complicated process, affecting solute diffusion, etc., and achieves low price, good biocompatibility, and good The effect of the application foreground

Inactive Publication Date: 2008-08-13
INST OF CHEM CHINESE ACAD OF SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there are still some problems in the alginate immobilization technology, such as the large particle size solid environment affects the diffusion of solutes in the capsule [Document 3: Chen Xiguang et al. A method for preparing chitin microsphere carrier. Chinese invention patent 96115714] , requires special capsule membrane materials (such as polylysine, highly deacetylated chitosan), complex process and high preparation cost [Document 4: Ma Xiaojun et al. A preparation method of microcapsules with immobilized yeast cells. Chinese invention patent 200310118155.7] etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alginate capsule immobilized with cell and preparation method thereof
  • Alginate capsule immobilized with cell and preparation method thereof
  • Alginate capsule immobilized with cell and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, preparation of calcium alginate capsules immobilized with yeast cells

[0029] The yeast in the logarithmic growth phase of the suspension culture is centrifuged to collect the thalline, and at 37 ° C with gelatin-calcium chloride solution (making the gelatin concentration after mixing is 15% (wt %), the calcium chloride concentration is 0.1mol / L ) and mix evenly, then drop into low-temperature hydroxy silicone oil (5°C), and cool for ten minutes to form a gelatin pellet with a particle size of 2.5-3.0mm, then add 0.5% (wt%) alginic acid to the pellet under stirring Film-forming reaction in sodium solution for 90 seconds, then filter out with a sieve, and continue to react in 0.1mol / L calcium chloride solution for 10 minutes under stirring to enhance the strength of the capsule membrane. Finally, filter with a sieve, wash three times with Tris buffer, and treat at a constant temperature of 37°C in Tris buffer to obtain calcium alginate encapsulated yeast (suc...

Embodiment 2

[0032] Example 2, preparation of calcium alginate capsules immobilized with Escherichia coli

[0033]Escherichia coli (E.coli JM109 (pKST11)) in the logarithmic growth phase of the suspension culture was centrifuged to collect the thalline, and mixed with gelatin-calcium chloride solution (so that the gelatin concentration after mixing was 25% (wt %), Calcium chloride concentration is 0.1mol / L) and mixed evenly, drop into low-temperature hydroxyl silicone oil (10°C), and cool for ten minutes to form gelatin balls with a particle size of 3.5-4.0mm, and then add the balls to 1.5 % (wt%) sodium alginate solution for film-forming reaction for 120 seconds, then filter out with a sieve, and continue to react in 0.1mol / L calcium chloride solution for 10 minutes under stirring to enhance the strength of the capsule membrane. Finally, it was filtered with a sieve, washed three times with Tris buffer, and treated at a constant temperature of 37°C in Tris buffer to obtain Escherichia col...

Embodiment 3

[0035] Example 3, preparation of alginate capsules immobilized with yeast cells

[0036] The yeast in the logarithmic growth phase of the suspension culture was centrifuged to collect the thalline, and mixed with gelatin-barium chloride solution (making the gelatin concentration 5% (wt%) and barium chloride concentration 0.05mol / L after mixing) at 37°C Mix evenly, drop into low-temperature liquid paraffin (2°C), and cool for ten minutes to form gelatin pellets with a particle size of 1.0-1.5mm, and then add the pellets to 0.5% (wt%) sodium alginate solution under stirring Film-forming reaction for 40 seconds, then filter out with a sieve, and continue to react in 0.05mol / L barium chloride solution for 10 minutes under stirring, so as to enhance the strength of the capsule membrane. Finally, it was filtered through a sieve, washed three times with acetate buffer, and treated at a constant temperature of 25°C in acetate buffer to obtain barium alginate-encapsulated yeast.

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses immobilization cellular alginate capsules and method for preparing the same. The immobilization cellular alginate capsule of the invention comprises capsules film which is prepared from alginate and liquid cavity in which cells are dispersed. The capsule has good biocompatibility, suitable film depth and the environment in the capsule is liquid state so the mass transfer problem of carrier prepared in traditional immobilization technology is improved greatly and all cells in the capsule can maintain activity in cultivating process to ensure availability of cell. Furthermore, larger capsule has evident advantage in large scale preparation and reclamation aspects and has good applications foreground.

Description

technical field [0001] The invention relates to a capsule and a preparation method thereof, in particular to a cell-immobilized alginate capsule and a preparation method thereof. Background technique [0002] Encapsulation is the process of encapsulating solids or liquids with natural or synthetic polymeric materials. The main features of this technology are to change the physical and chemical properties of active substances (phase state, solubility, etc.); to protect substances from environmental conditions; to shield taste, color and odor; to reduce the toxicity of substances; to control the release of active substances, etc. Biomacromolecules, microorganisms or cells encapsulated in capsules are blocked inside the capsule membrane, while nutrients and other small molecular substances can freely pass through the capsule membrane for material transfer, so as to achieve the purpose of catalysis, cultivation and immune isolation. Bioencapsulation technology has been widely u...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04
Inventor 戴鸿君徐坚张小莉梁松苗郭兴林
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap