Preparations quality control method containing radix dipsaci and pseudo-ginseng activity extracting combination
A quality control method and composition technology, applied in the field of quality control of natural medicines, can solve problems such as the lack of quality research of Sanqi compound preparations, achieve good reproducibility and reliability, good recovery rate, and quality control effects
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[0047] Example 1
[0048] 1.1 Expand conditions
[0049] Stationary phase: silica gel G plate (100×200mm, thickness 0.20-0.25mm, Qingdao Ocean Chemical Plant Branch).
[0050] Developing agent: The lower layer solution of chloroform-methanol-n-butanol-water (2:1:3:3) is used as the developing agent, the saturation time is 30 minutes, and it is developed at not less than 20°C.
[0051] Color development: spray with sulfuric acid ethanol solution (1→10), and heat immediately at 100~110℃ until the spots are clearly colored. 1.2 Solution preparation
[0052] Preparation of the test solution: Take 380 mg of the compound capsule content, place it in a 10 ml measuring flask, add methanol to the volume, ultrasonicate for 5 minutes, shake well, and filter to get it.
[0053] Preparation of the Reference Substance Solution of Mutong Saponin D Take 30 mg of Mutong Saponin D and put it in a 10ml measuring flask, add methanol to dissolve, dilute, make the volume constant, shake well, and get i...
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[0062] Example 2
[0063] 1. Apparatus and materials
[0064] Agilent 1100 series high performance liquid chromatograph, including binary gradient pump, column thermostat, UV detector; HW chromatography workstation (Shanghai Qianpu); ZRS-8G drug dissolution apparatus (Tianjin University Radio Factory); KQ2200DE medical numerical control Ultrasonic cleaner; needle filter (water film): 0.45μm, Germany MENBRANA company
[0065] Methanol (analytical purity), acetonitrile (chromatographic purity, Merk Company, Germany), distilled water (homemade).
[0066] 2. Chromatographic conditions
[0067] Column: Kromasil C 18 Column (250mm×4.6mm, 5um); Acetonitrile-water is the mobile phase, where A is acetonitrile, B is water, A+B=100%, using gradient elution; 0-20 minutes, 80%-60% B, 20-21 minutes, 60%-80% B, hold for 5 minutes, and record the chromatogram. The flow rate is 0.8ml / min; the detection wavelength is 203nm.
[0068] 3. Experimental method
[0069] 3.1 Solution preparation
[0070] ...
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[0086] Example 3
[0087] 1. Instruments and reagents
[0088] Instrument: Shimadzu UV-2401 spectrophotometer; Shimadzu MPS-2000 spectrophotometer; BRANSON200 ultrasonic cleaner (ULTRASONIC CLEANER, 50-60HZ); WH-1 mini vortex mixer (Shanghai Huxi Analytical Instrument Factory)
[0089] Reagents: methanol (analytical purity, Nanjing Chemical Reagent Factory); 77% sulfuric acid (concentrated sulfuric acid is superior grade, 23ml distilled water plus sulfuric acid 77ml); 8% vanillin ethanol solution (analytical purity, 800mg vanillin dissolved in 10ml In absolute ethanol, prepare before use).
[0090] 2. Test method
[0091] 2.1 Solution preparation
[0092] Preparation of test product solution Accurately weigh 15mg of the test product powder in the compound capsule and place it in a 100ml measuring flask, add methanol to a constant volume, ultrasonically assist the solution for 5 minutes, place it to room temperature, shake well, filter, and get it.
[0093] Preparation of the refer...
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