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Bone induction material and preparation method and application thereof

An osteoinductive and factor technology, applied in bone diseases, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of not protecting the inhibition and degradation of BMPs, and not effectively enhancing the biological activity of BMPs, and achieve protection from degradation. , Enhance biological activity, slow release trace effect

Inactive Publication Date: 2008-08-27
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Chinese patent document CN02114509.1 discloses a bone repair material with animal fibrin as a carrier, the material still has defects. It cannot protect BMPs from inhibition and degradation, and cannot effectively enhance the biological activity of BMPs

Method used

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  • Bone induction material and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0034] The rhBMP-2 sustained-release system is temporarily prepared during application and is composed of the following three components mixed in sequence. Component I is rhBMP-2 freeze-dried powder; component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH 9.0, where the concentration of fibrinogen in component II is 120 mg / ml, the molar ratio of fibrinogen to fibronectin is 10:1, and the molar ratio of fibronectin to heparin is 10:1. In component II, 1.2IU of fibrinogen is added to each 1mg of fibrinogen. Factor XIII; component III is a solution containing thrombin and calcium chloride, pH 9.0, the concentration of the thrombin in component III is 800IU / ml, the CaCl 2 The concentration in component III is 45umol / ml. During the preparation, add component II to component I and dissolve it in a 37°C water bath for 15 minutes to fully dissolve the rhBMP-2 lyophilized powder. The mass ratio of rhBMP-2 to heparin is 1:50, and then Add component III, s...

Embodiment 2

[0036] The rhBMP-2 sustained-release system is temporarily prepared during application and is composed of the following three components mixed in sequence. Component I is rhBMP-2 freeze-dried powder; component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH 8.5, where the concentration of fibrinogen in component II is 60 mg / ml, the molar ratio of fibrinogen to fibronectin is 5:1, and the molar ratio of fibronectin to heparin is 5:1. In component II, 0.65 IU of fibrinogen is added for every 1 mg of fibrinogen. Factor XIII; component III is a solution containing thrombin and calcium chloride, pH 8.6, the concentration of thrombin in component III is 450 IU / ml, the CaCl 2 The concentration in component III is 40umol / ml. During preparation, add component II to component I, and dissolve it in a 35℃ water bath for 25 minutes to fully dissolve the rhBMP-2 freeze-dried powder. The mass ratio of rhBMP-2 to heparin is 1:25, and then Add component III, shake...

Embodiment 3

[0038] The rhBMP-2 sustained-release system is temporarily prepared during application and is composed of the following three components mixed in sequence. Component I is rhBMP-2 freeze-dried powder; component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH 6.8, of which the concentration of fibrinogen in component II is 4 mg / ml, the molar ratio of fibrinogen to fibronectin is 1:1, and the molar ratio of fibronectin to heparin is 1:1. In component II, 0.3 IU of fibrinogen is added to each 1 mg of fibrinogen. Factor XIII; component III is a solution containing thrombin and calcium chloride, pH 6.9, the concentration of the thrombin in component III is 40IU / ml, the CaCl 2 The concentration in component III is 35umol / ml. During preparation, add component II to component I and dissolve it in a water bath at 33°C for 30 minutes to fully dissolve the rhBMP-2 freeze-dried powder. The mass ratio of rhBMP-2 to heparin is 1:1, and then Add component III, sh...

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Abstract

The invention discloses a bone induction material which comprises bone induction factors and carriers; the bone induction factors comprise bone morphogenetic proteinbmp or basic fiberblast growth factors, and the carriers form gel matrix through the interaction among fibernogen, fibernectin, heparin, fiber protein stabling factor, thrombin and calcium chloride; the bone induction factor is embedded in the gel matrix. The invention further discloses a preparation method and application of bone induction material, which can release the bone morphogenetic proteinbmp slightly, efficiently and stably, and can improve the biological activity of bone morphogenetic proteinbmp.

Description

【Technical Field】 [0001] The invention relates to a bone tissue repair material, in particular to an osteoinductive material and a preparation method and application thereof. 【Background technique】 [0002] Bone is the most common tissue repaired by transplantation after blood. In the United States, there are more than 500,000 bone transplants each year. There are 2.2 million bone transplant operations performed in orthopedics, neurosurgery and dentistry for repairing bone defects every year worldwide. The application in spine surgery accounts for about half of the total number of bone transplant operations. With the aging of the population, the number of cases of spinal degenerative diseases that need to be treated has increased sharply, and the application of bone transplantation in spinal surgery is rapidly increasing. Bone transplantation is a surgical method used to treat bone defects that exceed the healing capacity of the body and perform bone fusion between vertebral bodi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/54A61K38/18A61K47/42A61P19/08
Inventor 韩大庆刘伟强敖强
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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