Bone induction material and preparation method and application thereof

An osteoinductive and factor-based technology, applied in bone diseases, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of not being able to protect BMPs from inhibition and degradation, and not being able to effectively improve the biological activity of BMPs, so as to achieve protection from degradation , enhance biological activity, improve the effect of biological activity

Inactive Publication Date: 2011-05-25
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Chinese patent document CN02114509.1 discloses a bone repair material with animal fibrin as a carrier, the material still has defects. It cannot protect BMPs from inhibition and degradation, and cannot effectively enhance the biological activity of BMPs

Method used

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  • Bone induction material and preparation method and application thereof
  • Bone induction material and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The rhBMP-2 sustained-release system is temporarily prepared at the time of application, and is formed by mixing the following three components in sequence. Component I is rhBMP-2 lyophilized powder; Component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH 9.0, wherein the concentration of fibrinogen in Component II is 120mg / ml, the molar ratio of fibrinogen to fibrinogen is 10:1, the molar ratio of fibrinogen to heparin is 10:1, in component II, every 1mg of fibrinogen corresponds to adding 1.2IU of Factor XIII; Component III is a solution containing thrombin and calcium chloride, pH9.0, the concentration of said thrombin in component III is 800IU / ml, said CaCl 2 The concentration in fraction III is 45umol / ml. During preparation, add component II to component I, and put it in a water bath at 37°C for 15 minutes to dissolve the rhBMP-2 lyophilized powder. The mass ratio of rhBMP-2 to heparin is 1:50, and then Add component III, shake...

Embodiment 2

[0036] The rhBMP-2 sustained-release system is temporarily prepared at the time of application, and is formed by mixing the following three components in sequence. Component I is rhBMP-2 freeze-dried powder; Component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH 8.5, wherein the concentration of fibrinogen in Component II is 60mg / ml, the molar ratio of fibrinogen to fibrinogen is 5: 1, the molar ratio of fibrinogen to heparin is 5: 1, in component II, every 1mg of fibrinogen corresponds to adding 0.65IU of Factor XIII; component III is a solution containing thrombin and calcium chloride, pH8.6, the concentration of said thrombin in component III is 450IU / ml, said CaCl 2 The concentration in fraction III is 40umol / ml. During preparation, add component II to component I, and place it in a water bath at 35°C for 25 minutes to dissolve the rhBMP-2 lyophilized powder. The mass ratio of rhBMP-2 to heparin is 1:25, and then Add component III, sh...

Embodiment 3

[0038] The rhBMP-2 sustained-release system is temporarily prepared at the time of application, and is formed by mixing the following three components in sequence. Component I is rhBMP-2 lyophilized powder; Component II is a solution containing fibrinogen, fibronectin, heparin and factor XIII, pH6.8, wherein the concentration of fibrinogen in Component II is 4mg / ml, the molar ratio of fibrinogen to fibrinogen is 1:1, the molar ratio of fibrinogen to heparin is 1:1, in component II, every 1mg of fibrinogen corresponds to adding 0.3IU of Factor XIII; Component III is a solution containing thrombin and calcium chloride, pH6.9, the concentration of said thrombin in component III is 40IU / ml, said CaCl 2 The concentration in fraction III is 35umol / ml. During preparation, add component II to component I, and dissolve in a water bath at 33°C for 30 minutes to fully dissolve the rhBMP-2 lyophilized powder. The mass ratio of rhBMP-2 to heparin is 1:1, and then Add component III, shak...

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Abstract

The invention discloses a bone induction material which comprises bone induction factors and carriers; the bone induction factors comprise bone morphogenetic proteinbmp or basic fiberblast growth factors, and the carriers form gel matrix through the interaction among fibernogen, fibernectin, heparin, fiber protein stabling factor, thrombin and calcium chloride; the bone induction factor is embedded in the gel matrix. The invention further discloses a preparation method and application of bone induction material, which can release the bone morphogenetic proteinbmp slightly, efficiently and stably, and can improve the biological activity of bone morphogenetic proteinbmp.

Description

【Technical field】 [0001] The invention relates to bone tissue repair materials, in particular to an osteoinductive material and its preparation method and application. 【Background technique】 [0002] Bone is next to blood the most common tissue to be repaired by transplantation. In the United States, more than 500,000 bone grafts are performed each year. Worldwide, up to 2.2 million bone grafts are performed annually in orthopedics, neurosurgery and dentistry to repair bone defects. The application in spine surgery accounts for about half of the total number of bone graft operations. The use of bone grafting in spine surgery is increasing rapidly as the population ages and the number of cases of degenerative spinal disease requiring treatment soars. Bone grafting is a surgical method used to treat bone defects that exceed the body's healing capacity and to perform bony fusion between vertebrae. In spinal surgery, the problem of maintaining spinal stability after spinal s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/54A61K38/18A61K47/42A61P19/08
Inventor 韩大庆刘伟强敖强
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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