Producing method of novel tobacco fermenting enzyme preparations

Abstract

The invention relates to a novel production method of a tobacco-leaf fermentation enzymatic preparation, which aims at solving the technical problems how to improve tobacco-leaf fermentation quality and realize the activity protection of the tobacco-leaf fermentation enzymatic preparation in the tobacco-leaf spontaneous fermentation field. The enzymatic preparation is composed of glucose oxidase, chlorophyll oxidase, carotenoid oxidase, protease, and nicotine-degradation enzyme. Fresh tobacco leaves are processed through cell disruption, and processed through secondary fractional precipitation by (NH4)2SO4 to obtain crude enzyme liquid; enzyme molecules adopt Ca<2+> and Mg <2+> to act metal ion exchange to complete molecular modification; macromolecule combining modification is completed by adopting sucrose oligomer of 0.01 percent, so that the half-life of the enzymatic preparation can be prolonged, and the heat-resisting ability thereof can be increased markedly. Experimental results which are obtained by using the enzymatic preparation show that the nicotine is reduced 9.3 percent, total nitrogen is reduced 5.7 percent, protein is reduced 7.1 percent, smoke total particulate matter is reduced 8.4 percent, the quantity of the tar is reduced 5.1 percent, the nicotine content in smoke is reduced 28.0 percent, and carbon monoxide is reduced 1.6 percent. The enzymatic preparation is used in the secondary moistening process before threshing and re-drying of the tobacco leaves.

Description

technical field [0001] Tobacco leaves ferment naturally. Background technique [0002] In the process of tobacco leaf modulation, fully fermenting the tobacco leaves after primary roasting and re-curing under suitable temperature and humidity conditions is an extremely important technical link to improve and improve the quality of tobacco leaves. Therefore, the research on the fermentation mechanism has been the focus of domestic and foreign scholars and cigarette manufacturers for many years. One of the important research areas is the elucidation and application of the enzyme mechanism of tobacco leaf fermentation. As early as 1950 (Frankenburg, W.G.), some scholars put forward the theoretical framework that tobacco fermentation is catalyzed by enzymes. With the deepening and accumulation of research, it is now generally believed that the oxidoreductases contained in tobacco leaves themselves dominate the fermentation, and this fermentation process is the result of the jo...

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Problems solved by technology

[0005] A common feature of the above documents and patents is that exogenous (micro) biological enzymes are directly mixed in a specific ratio and then ap

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