Method for separating effective constituent butenolide II from astraolylis lancea formalyrata volatile oil

Abstract

The invention discloses a method for separating effective component, atractylenolide II, from Rhizoma Atractylodis Macrocephalae volatile oil. The method includes pulverizing rhizome of dried Rhizoma Atractylodis Macrocephalae, extracting in medium and low polar solvents, and concentrating to obtain tan volatile oil; standing for a certain time, extracting the volatile oil with mixed solution prepared from n-hexane, ethyl acetate, ethanol and water at a certain ratio, collecting upper layer phase, and concentrating to obtain the refined volatile oil; and gradient eluting with mixed solvent of petroleum ether and ethyl acetate at a certain ratio with column chromatography, collecting correspondent fraction, concentrating, and recycling solvent to obtain atractylenolide II. The method performs pretreatment on Rhizoma Atractylodis Macrocephalae volatile oil coarse product by extracting and oxidizing before separating through column chromatography to have simple process and low cost, increased yield and purity of atractylenolide II, and avoids the disadvantages in the conventional post-treatment steps such as crystal loss caused by recrystallization, etc.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a method for separating the active ingredient atractylodes lactone II from the volatile oil of atractylodes rhizome. Background technique [0002] Atractylodes macrocephala koidz (Atractylodes macrocephala koidz), also known as Yushu, Dongzhu, Zheshu, etc., is a perennial herb and a medicinal plant of the Atractylodes genus in the family Asteraceae. Its rhizomes can be used as medicine. Atractylodes macrocephala is warm in nature, sweet and bitter in taste, and belongs to the spleen and stomach meridians, and has the functions of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant, and preventing miscarriage. It is mainly produced in Zhejiang, Anhui and other places, with the largest output in Yinxian and Xinchang of Zhejiang. It is mainly used for spleen deficiency, indigestion, abdominal distension and dia...

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Problems solved by technology

[0007]The content of Atractylodes macrolide II in Atractylodes macrocephala is only 0.07% (Li Wei. Effective substances and quality research of Atractylodes macrocephala. Nanjing: Nanjing University of Traditional Chinese Medicine, 2001, 38.) , and it is difficult to separate

The separation method commonly used in the prior art is high-speed counter-current chromatographic separation (Chunxia Zhao, Chaohong He.Preparative isolation and purification of atractyl on and atractylenolide III from the Chinese medicinal plant Atractylodesmacrocephala by high-speed counter-current chromatography.J.Sep.Sci. , 2006, 29: 1630-1636.) and column chromatography chromatographic separation (Li Wei. Effective substances and quality research of Atractylodes macrocephala. Nanjing: Nanjing University of Traditional Chinese Medicine, 2001, 29-79.), but the former is in the industrial production at this stage There is a certain degree of difficulty. The latter generally needs to be separated by subsequent means such as recrystallization to obtain a relatively pure substance, which may lose a part of the substance. Not only that, but the separation by column chromatography has a low yield and purity of the substance

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