Isolated culture method for anaerobic microorganism

Abstract

The invention relates to an anaerobe isolating culture method. Throughout the operational process, target microbes are prevented from contacting oxygen, and the operating cost is relatively low. The method is in particular applicable for high temperature anaerobes and strict anaerobes with the growth temperature above 50 DEG C and strict requirement for anaerobic environment difficult to be isolated. In the method, oxygen-free workstations are used only during partial operation of isolation of anaerobes. When anaerobes are cultured, common incubators can be used without anaerobic environment, thus preventing one oxygen-free workstation from being used for bacteria in just one growth temperature. The method is similarly applicable for bacteria with the culture temperature above the upper temperature limit of the oxygen-free workstations.

Description

field of invention [0001] The present invention relates to a method for separating anaerobic microorganisms, in particular to an indispensable new method for extremophile physiological ecology research, natural ecology research, genetic engineering research, life science research, pathology research, marine geological research and environmental pollution control. method. Background technique [0002] In natural ecosystems, microorganisms play a very important role. In pollution control, microorganisms can decompose many organic or inorganic pollutants that cannot be removed by other methods, and the cost is low; in geological research, the kinship of specific types of extremophiles in different regions may mark the previous location of these regions relation. However, microorganisms are often based on flora as a unit, and a variety of microorganisms live together. Therefore, the isolation and pure culture of microorganisms is a problem that must be solved first in the stud...

AI Technical Summary

Problems solved by technology

[0006] 1) Using an anaerobic workstation to isolate and cultivate bacteria can ensure that the target bacteria are isolated from oxygen during the whole process of isolation. However, the upper limit of the culture temperature generally set in the current anaerobic workstation is 50 ° C, which is difficult to give thermophilic anaerobic bacteria. Oxygen bacteria provide sufficient culture temperature

Moreover, even if it is desired to isolate and cultivate bacteria in a growth environment below 50°C, one anaerobic workstation can only set one cultivation temperature, and multiple anaerobic workstations are required for the isolation and cultivation of microorganisms at different temperatures, which greatly increases the cost of the experiment

At the same time, it is difficult to provide a strict aseptic environment in the anaerobic workstation, and it is easy to contaminate the petri dishes with each other during cultivation.

[0007] 2) Using anaerobic tank, anaerobic bag and rolling tube to separate anaerobic microorganisms can provide bacteria with a culture temperature above 50°C, but the whole operation process is exposed to the air, and the target anaerobic bacteria will come into contact with oxygen, resulting in the growth of the target bacteria. body death

[0008] 3) The document "An Improved Method for Isolating and Cultivating Sulphate-Reducing Bacteria" (Wan Haiqing, Journal of Applied and Environmental Biology, 2003, 9(5): 561-562) introduces the separation of anaerobic microorganisms by the stacked dish interlayer method, However, during the coating operation of this method, the bacteria will be exposed to the air for a short time

[0009] 4) The document "Evaluation of a new anaerobic culture dish-OxyplateTM" (Zhao Hu et al., Shanghai Journal of Medical Laboratory, 2002, 06) introduces the method of utilizing the OxyplateTM culture dish to separate anaerobic bacteria. Exists in the process of coating operation, the bacteria are exposed to the air for a short time, resulting in the death of the bacteria

[0010] 5) A petri dish suitable for anaerobic culture is patented (patent number: 200420074957.2). This patent is aimed at the discovery and cultivation of anaerobic pathogenic bacteria in medical grass-roots institutions. Pathogenic bacteria often live in a non-strictly anaerobic environment In the case of using this culture dish to isolate bacteria with a short oxygen resistance time, it may cause the death of the bacteria

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