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Iota-carrageenan even oligosacchride alcohol monomer and preparation thereof

An even-numbered oligosaccharide, iota-technology, applied in the field of iota-carrageenan even-numbered oligosaccharide alcohol monomer and its preparation, to achieve the effects of no three-waste pollution, stable product performance, and high production efficiency

Inactive Publication Date: 2008-10-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are patent reports that acid method degradation is prepared K-carrageenan even-numbered oligosaccharide monomer and agar gum even-numbered oligosaccharide monomer (Yu Guangli etc., CN100999537; Yu Guangli etc., CN101012249), but do not see adopting at present Preparation of iota-carrageenan even-numbered oligosaccharide alcohol monomer by reducing acid degradation

Method used

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  • Iota-carrageenan even oligosacchride alcohol monomer and preparation thereof
  • Iota-carrageenan even oligosacchride alcohol monomer and preparation thereof
  • Iota-carrageenan even oligosacchride alcohol monomer and preparation thereof

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Embodiment 1

[0014] Dissolve iota-carrageenan into a 1-5% (W / W) aqueous solution with hydrochloric acid with a concentration of 0.1-0.5mol / L, then add sodium borohydride, the concentration of sodium borohydride in the aqueous solution is 0.2-2.0mol / L, the iota-carrageenan in the aqueous solution was degraded at 50-95°C for 30-480 minutes under stirring, and filtered, and the filtrate was neutralized with aqueous sodium hydroxide solution, concentrated by rotary evaporation, and precipitated with absolute ethanol. Wash and dissolve the precipitate in water, then use 0.2mol / L ammonium bicarbonate aqueous solution as the mobile phase, use Superdex 30 packing for column chromatography, detect with differential detector, collect according to the elution peaks, concentrate, and freeze-dry to obtain The iota-carrageenan even-numbered oligosaccharide alcohol monomer of the present invention.

[0015] The hydrochloric acid described in the present embodiment can use sulfuric acid, nitric acid, for...

Embodiment 2

[0017] Dissolve iota-carrageenan in water into a 0.5-5% (W / W) aqueous solution, add 2-20 grams of hydrogen-type cation exchange resin, and then add 4-methylmorpholine borane to make 4-methylmorpholine borane The concentration of alkane is 0.2-2.0mol / L. The above aqueous solution is degraded under stirring at 50-95°C for 30-480 minutes and then filtered. The filtrate is neutralized with ammonium hydroxide. After filtration, it is concentrated by rotary evaporation and precipitated with acetone. , washing, then use 0.2mol / L ammonium bicarbonate aqueous solution as mobile phase, carry out column chromatography separation with BioGel P10 filler, detect with differential detector, merge and collect by elution peak, concentrate, freeze-dry to obtain final product of the present invention iota-carrageenan even-numbered oligosaccharide alcohol monomer.

[0018] The hydrogen type cation exchange resin described in the present embodiment is 732 resins, Dowex AG50W X4 resin or Amberlite ...

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Abstract

Disclosed is an even oligosaccharide alcohol monomer of iota-carrageenan, with polymerization degree at 2-20 and molecular formula as C12n+12H16n+20O15n+16S2n+2Na2n+2 (n=0-9). The preparation includes the following steps: degrading iota-carrageenan in acid or hydro-strong cation resin with the existence of reducer under 50-95 DEG C for 30-480min; filtrating and then neutralizing the filtrate through alkaline; condensing; sedimenting through alcohol; washing; proceeding Superdex 30 or BioGel P10 chromatographic column separation, with aqueous ammonium bicarbonate solution as mobile phase; detecting through a differential detector; combining and collecting according to leaching peak; condensing; freezing and drying. The preparation method is simple in process and low in cost, with high yield of oligosaccharide and no pollution(gas,liquid and solid).The oligosaccharide alcohol monomer of iota-carrageenan is stable in performance amd can be used as reagent in the standardization of the molecular weight of sulfated oligosaccharide.

Description

technical field [0001] The invention relates to an iota-carrageenan oligosaccharide, in particular to an iota-carrageenan even-numbered oligosaccharide alcohol monomer with a polymerization degree of 2-20 and a preparation method thereof. Background technique [0002] Carrageenan is a linear polymer compound composed of galactose and galactose derivatives. Carrageenan mainly has three types: kappa, lambda and iota, among which iota-carrageenan is composed of 1,3-linked-4-sulfate-β-D-galactose (G4S) and 1,4-linked-2-sulfuric acid A linear polyanionic polymer formed by alternating linkages of 3,6-intraether-α-D-galactose (A2S). As a natural sulfated polysaccharide compound, carrageenan has been widely used in the fields of food, medicine and chemical industry. In recent years, many important biological functions and physiological activities of carrageenan oligosaccharides and their derivatives have been gradually recognized by people, and have been shown in anti-tumor, anti-...

Claims

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Application Information

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IPC IPC(8): C07H11/00C07H3/06C08B37/00
Inventor 于广利杨波赵峡焦广玲郝翠
Owner OCEAN UNIV OF CHINA
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