Method for continuous flow upstream abstraction purification of glycyrrhiza hypo-glycosides in glycyrrhizic acid biotransformation solution

Abstract

The invention relates to a method for continuously countercurrent extracting and purifying glycyrrhetinic glycoside in a glycyrrhizin biotransformation solution. The method is characterized in that: an extractant is added from the lower part of a turnplate tower; the glycyrrhizin biotransformation solution with a PH value of between 3.0 and 6.0 is added from the upper part of the turnplate tower,is subjected to continuous countercurrent liquid-liquid extraction at a temperature of between 30 and 60 DEG C and kept stand; an extraction phase is delaminated; and the extractant is separated and reclaimed for recycling, thereby obtaining a dried product of the glycyrrhetinic glycoside. The invention adopts a method of continuous countercurrent extraction and combines a method for adjusting the PH value of the glycyrrhizin biotransformation solution and controlling an extraction temperature, thereby realizing the rapid and effective separation of the glycyrrhetinic glycoside in a glycyrrhizin biotransformation system; and a purity of a separated glycyrrhetinic glycoside product reaches over 93.8 percent and a yield of the separated glycyrrhetinic glycoside product reaches over 92.5 percent.

Description

technical field [0001] The invention relates to an extraction and purification process of biotransformation products, in particular to a method for continuous countercurrent extraction and purification of glycyrrhizin in glycyrrhizic acid biotransformation solution. Background technique [0002] Glycyrrhetin, namely 18β-monoglucuronyl glycyrrhetinic acid (3-Monoglucuronyl-Glycyrrhetinic Acid, 3β-D-(monogluronyl) 18β-glycyrrhetic acid, referred to as GAMG), is a derivative of glycyrrhizic acid. Glycyrhetic acid (18β-GA) and a molecule of β-D-glucuronic acid (β-D-glucuronic acid, β-D-GluA). Under the catalysis of enzyme or acid, glycyrrhizic acid can be hydrolyzed to lose a glucuronic acid group to form glycyrrhetin. The hydrolysis of glycyrrhizic acid is shown in the attached figure 1 shown. [0003] Glycyrrhetin has inhibitory effect on hyaluronidase activity, also has inhibitory effect on contact dermatitis induced by m-trinitrobenzene chloride, can also inhibit platelet ...

AI Technical Summary

Problems solved by technology

[0007] Glycyrrhizic acid biotransformation system is more complex and special than the countercurrent extraction system disclosed above

Glycyrrhizic acid and glycyrrhizin have relatively large surface activity, so when extracting glycyrrhizin from glycyrrhizic acid biotransformation solution by liquid-liquid extraction, severe emulsification is likely to occur, which seriously hinders the separation of glycyrrhizin molecules in the aqueous and organic phases. The transfer between them, and the emulsified layer is not easily damaged, which not only prolongs the time for the extraction to reach equilibrium, but also causes the entrainment of organic solvents in the emulsified layer, and at the same time causes the residual loss of glycyrrhetin in the emulsified layer, which eventually leads to the extraction of glycyrrhetin The efficiency is reduced, and these problems are expected to be improved by the countercurrent extraction method. At present, there are no publications or reports on the rapid purification of glycyrrhetin based on the continuous countercurrent extraction of the rotating disk column at home and abroad.

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