Panax notoginseng saponins ST-4, and medicinal composition, preparation and use thereof
A technology of notoginseng saponin and ST-4, which is applied in the field of pharmaceutical compounds and drugs, can solve the problems of inhibitory effect that have not been reported before, and achieve the effect of reducing infectivity
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Embodiment 1
[0031]Embodiment 1. Separation and extraction of a new dammarane type triterpene saponin Notoginsenoside ST-4 (notoginsenoside ST-4):
[0032] 5kg Panax notoginseng was purchased in Yunnan, crushed into granules, wrapped with double gauze, placed in a pressure cooker, controlled at a high temperature of 120°C, and steamed for 12 hours to obtain cooked Panax notoginseng. Reflux extraction with industrial ethanol (5L×4), recover the solvent under reduced pressure and concentrate to obtain 778g of total extract. Dissolve the extract in water, perform column chromatography twice with D101 macroporous adsorption resin (90×8.5 cm), first elute with water until the Molish reaction of the effluent is negative, and then elute with industrial methanol to obtain 440 g of crude saponin.
[0033] The crude saponin was mixed with about 500 g of silica gel (200-300 mesh), and subjected to column chromatography on silica gel (200-300 mesh), eluted with chloroform-methanol-water (85:15:1), and...
Embodiment 2、 3
[0044] Example 2, the effect test of notoginsenoside ST-4 (notoginsenoside ST-4) on the infection of Vero cells by herpes simplex virus type I (HSV-1):
[0045] (1) Materials:
[0046] 1. Equipment: imported 24-well culture plate (flat bottom), inverted microscope, CO 2 Cell incubator, micro-adjustable sample injector, medium is MEM (containing 250U / ml blue chain double antibody), adjust pH to 7.0-7.2 with sodium bicarbonate before sterilization and filtration, and sterilize calf serum at 56°C for 30min Live complement, sterilized and aliquoted; the digestive solution is 5% trypsin 5ml+0.01mol / L (pH 7.2) PBS 93ml, sterilized and aliquoted, and stored at low temperature.
[0047] 2. Viruses and cell strains: Herpes simplex virus type 1 strains are from ATCC (VR733) in the United States and are kept by the applicant's laboratory. MRC-5 cells were purchased from the Cell Bank of the Chinese Academy of Sciences and kept by the applicant's laboratory. The MRC-5 cell line comes f...
Embodiment 3
[0072] Tablet: 10 mg of the compound Panax notoginsenoside ST-4 obtained in Example 1, 180 mg of lactose, 55 mg of starch, and 5 mg of magnesium stearate;
[0073] Preparation method: mix the compound, lactose and starch, moisten it evenly with water, sieve the wetted mixture and dry it, then sieve it, add magnesium stearate, then press the mixture into tablets, each tablet weighs 250mg, and the compound content is 10mg .
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