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Application of butein in preparing the medicine for preventing the blood vessel from regenerating

A technology of angiogenesis and Butea urea, which is applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problems of no angiogenesis drugs, etc.

Inactive Publication Date: 2009-01-07
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous reports have shown that Butea sinensis can directly inhibit tumor cell proliferation and has anti-tumor activity, and it also has anti-diabetic syndrome and anti-inflammatory effects. Inhibit tumors, and there is no report as an angiogenesis drug

Method used

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  • Application of butein in preparing the medicine for preventing the blood vessel from regenerating
  • Application of butein in preparing the medicine for preventing the blood vessel from regenerating
  • Application of butein in preparing the medicine for preventing the blood vessel from regenerating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Butein inhibits HUVEC cell proliferation

[0029] Purpose and principle: MTS assay was used in the cell proliferation experiment. The MTS assay is an assay that uses a colorimetric method to determine the number of cell proliferation in living cells. NADPH dehydrogenase converts MTS tetrazolium salt compound into a colored substance soluble in tissue culture fluid, and its color depth is highly correlated with the number of living cells of the cell line within a certain range. This model can be used to evaluate the effect of drugs on cell proliferation.

[0030] Method: Divide 1×10 5 Each / ml HUVEC cell suspension was inoculated in a 96-well plate, and the culture medium containing different concentrations of butycin were added to act for 48 hours, and then a chromogenic reagent was added to each well, and incubated for 3 hours, and then used a microplate reader at 490nm. Read the light absorbance in each well.

[0031] Results and evaluation: Compared wit...

Embodiment 2

[0032] Example 2: Butea chinensis inhibits scratch migration of HUVEC cells in vitro

[0033] Purpose and principle: Cell migration test is also called in vitro scratch test. In this experiment, scratches were made on the overgrown monolayer endothelial cells in the culture plate, observed under a microscope, and the migration of cells at the edge of the scratches to the scratched site was recorded to evaluate the effect of drugs on the migration ability of endothelial cells.

[0034] Methods: Cells were seeded in 6-well plates. When the cells grow to more than 90%, draw a line in each well with a 1ml tip of a micropipette. Wash the crossed out dead cells with PBS, then add VEGF 165 and medium containing different drug concentrations in 5% CO 2 Incubate in an incubator at 37°C for about 24 hours until VEGF 165 The group is full.

[0035] Result and evaluation: Observed and photographed under a microscope, the results are: A is the control group; B is 10nM Butacein. Can i...

Embodiment 3

[0036] Example 3: Butea chinensis inhibits migration of HUVEC cells in Boyden chamber

[0037] Purpose and principle: Millicell (Millopore) chamber was used in the Boyden chamber migration assay modified by endothelial cells. The modified Boyden chamber is a kind of petri dish that can be inserted into the cell culture plate. The bottom layer has a polycarbonate filter membrane with a pore size of 8 μm. Under the induction of growth factors, the cells can permeate the side of the membrane and adhere to the medium containing high concentration of The other side of growth factors. This experiment can detect the effect of drugs on the ability of endothelial cells to resist migration.

[0038]Method: Add gelatin to the 24-well plate and incubate at 37°C for 30 minutes, discard the gelatin, and wash three times with PBS. Add serum and VEGF to the 24-well plate outside the chamber 165 Endothelial cell culture medium. Insert cells into the small chamber, then add different concen...

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Abstract

The invention relates to a new application of butein, particularly to an application of butein in preparing angiogenesis inhibitory drugs for treating angiogenesis-dependent diseases such as breast cancer, colon cancer, lymphomata, acute myeloid leukemia, rheumatoid arthritis, psoriasis, diabetes syndrome, hemangioma, etc. The butein has distinct inhibitory action in both human umbilical vein endothelial cells (HUVEC) proliferation experiment, HUVEC migration experiment, HUVEC invasion experiment, human microvascular endothelial cells (HMEC-1) canalization experiment, chick embryo allantois angiogenesis, etc.; and can be used in preparing angiogenesis inhibitory drugs.

Description

technical field [0001] The present invention relates to the new application of Butein, in particular to the application of Butein in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is the process of generating new blood vessels on the basis of existing blood vessels. The process of angiogenesis includes five steps: 1. Generation of angiogenic stimulators; 2. Changes in the angiogenic phenotype of resting endothelial cells, which are activated; 3. Endothelial cell invasion, basement membrane degradation; 4. Extracellular Matrix remodeling, proliferation and movement; 5. Endothelial cell aggregation, formation of new microvessels, replenishment of perivascular cells and acquisition of new matrix membrane. Normally, angiogenesis occurs in the embryo and late embryonic development, reproductive cycle and wound healing period, and is regulated by various related factors, and is in a state of dynamic balance; if this state is out o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
Inventor 易正芳张京赖力张晓丽卢彬彬易庭芳刘明耀
Owner EAST CHINA NORMAL UNIV
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