Thalassia hemprichii extract and preparation method and use thereof

A technology of Thalias algae and extracts, applied in the field of preparation of antiviral drugs, can solve problems such as undeveloped economic value, and achieve strong anti-herpes simplex virus HSV-I virus activity, strong inhibition, and good insect antifeedant activity Effect

Inactive Publication Date: 2009-01-07
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Thalesia (also known as Thalesia) belongs to the genus Thalesia of the family Hydrochardaceae, and is the d

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Thalassia hemprichii with an air-dried dry weight of 4Kg was used as a raw material, chopped and soaked in about 35 L of ethanol organic solvent at room temperature for 3 times, each time for 5 days, and the extract was concentrated under reduced pressure to obtain a crude extract. The crude extract (70g) was suspended and dissolved in water (1L), extracted three times with chloroform (600mL) and n-butanol (500mL) successively, the aqueous layer was collected and concentrated under reduced pressure, and 23g of the concentrated aqueous layer was subjected to macroporous Resin (D101 macroporous adsorption resin) atmospheric column (atmospheric pressure glass column, diameter 9cm, length 1.8m) chromatography, using water-methanol solvent system as eluent, gradient from volume ratio 100:0 to 0:100 After elution, the fractions eluted with methanol were collected and concentrated under reduced pressure to obtain crude fractions. Rinse the crude components with methanol, colle...

Embodiment 2

[0021] Collect vero cells in the logarithmic growth phase and inoculate them in 96-well culture plates, the number of cells per well is 1.0×10 5 / 100μL, placed in 5%, CO 2 Incubator cultivation. The next day, suck out the growth liquid in the microwells, add 50 μL of the diluted Thaleiella extract solution in Example 1 to each well, immediately add 50 μL of HSV-I virus use solution, and set normal cell control and virus control at the same time, 37 ° C , 5% CO 2 Incubator cultivation. Observe the cytopathic effect (CPE), observe the cell growth every day, and express the cytopathic condition by -, +, ++, +++, ++++. The results show that the compound of the present invention has a maximum nontoxic concentration of 25ug / ml to vero cells, and it inhibits the IC of herpes simplex virus type I lesions. 50 The value is 6.25ug / mL.

Embodiment 3

[0023] HepG2 cells in the logarithmic growth phase were collected and seeded in 96-well culture plates, with the number of cells per well being 1.0×10 5 / 100μL, placed in 5%, CO 2 Incubator cultivation. The next day, the growth liquid in the microwells was aspirated, and 50 μL of the diluted Tailai grass extract solution in Example 1 was added to each well, and 50 μL of the CVB3 virus use solution was immediately added. %CO 2 Incubator cultivation. Observe the cytopathic effect (CPE), observe the cell growth every day, and express the cytopathic condition by -, +, ++, +++, ++++. The results show that the maximum non-toxic concentration of the compound of the present invention to hela cells is 200 μg / mL, and when the concentration is 100 μg / mL, it can inhibit more than half of the pathological changes of the virus.

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PUM

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Abstract

The invention relates to a Thalassia hemprichii extract, and a preparation method and an application thereof. The preparation method comprises soaking Thalassia hemprichii with an organic solvent to obtain a crude extract, performing sequential liquid extraction of the crude extract with chloroform or ethyl acetate and n-butanol, collecting water layer, performing macroporous resin normal pressure column chromatography, eluting with a water-methanol solvent system or a water-ethanol solvent system, collecting methanol or ethanol eluate, rinsing with methanol, and collecting component indissoluble in methanol and having strong ultraviolet absorption at ultraviolet wavelength of 254nm to obtain the product. The inventive Thalassia hemprichii extract has strong anti-herpes simplex virus (HSV-1) activity, in vitro anti-CVB3 virus activity, insect antifeedant activity, and strong bryozoan (Bugula neritina) larvae attachment inhibitory activity; and can be used for preparing antiviral drugs, biological pesticides and ocean green anti-fouling agents.

Description

technical field [0001] The invention relates to a Thalassia hemprichii extract, and also relates to a preparation method of the extract and its application in the preparation of antiviral drugs, biological pesticides and marine antifouling agents. Background technique [0002] Thalesia (also known as Thalesia) belongs to the genus Thalesia of Hydrocharidaceae, and is the dominant species in seagrass beds in the South my country Sea, but its economic value has not yet been developed. Contents of the invention [0003] The purpose of the present invention is to extract the compound with medical value from Thaleisis, so as to realize the economic value of Thealais. [0004] Through organic solvent extraction, atmospheric pressure column chromatography separation technology, etc., we isolated an extract from Thalais algae whose main component is a flavonoid glycoside compound, which has anti-virus, insect antifeedant activity and anti-marine fouling organisms The effect of la...

Claims

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Application Information

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IPC IPC(8): A61K36/02A61P31/12A61P31/22A01N63/00A01P3/00A01P7/04
Inventor 漆淑华
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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