Magnetic carbon sphere of surface finished C8 alkyl chain, preparation and application thereof

A technology of surface modification and alkyl chains, applied in the field of magnetic carbon spheres and its preparation, can solve problems such as cumbersome operations, and achieve good practical value and application prospects

Inactive Publication Date: 2009-01-14
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Professor Yang Pengyuan's group successfully applied zeolite nanoparticles to the enrichment of trace peptides[5-7], but this method requires high-speed centrifugation to separate the sample and zeolite mixture, and the operation is relatively cumbersome

Method used

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  • Magnetic carbon sphere of surface finished C8 alkyl chain, preparation and application thereof
  • Magnetic carbon sphere of surface finished C8 alkyl chain, preparation and application thereof
  • Magnetic carbon sphere of surface finished C8 alkyl chain, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the synthesis of the magnetic carbon sphere of surface modification C8 alkyl chain

[0027] The synthesis of magnetic carbon spheres with surface-modified C8 alkyl chains is divided into three steps.

[0028] First, the aminoferroferric oxide magnetic nanoparticles were synthesized by hydrothermal method: 1.0 g FeCl 3 ·6H 2 O was dissolved in 30 mL of ethylene glycol, and magnetically stirred for 0.5 h to obtain a yellow transparent solution. Then 4.0 g of anhydrous NaAc was added, and after magnetic stirring for 0.5 h, a brown-yellow transparent solution was obtained. The resulting solution was transferred into a 200 mL Teflon-lined stainless steel reaction kettle. Put it in an oven at 200°C for 12 hours. After cooling to room temperature, the product was repeatedly washed with deionized water five times to remove water-soluble impurities such as sodium acetate and ethylene glycol, and finally the product was vacuum-dried at 50°C for future use.

[0...

Embodiment 2

[0031] Example 2, Magnetic carbon spheres with surface-modified C8 alkyl chains used for peptide enrichment

[0032] (1) Enzymatic hydrolysis of protein solution:

[0033] Take 1.0 mg protein sample, including bovine serum albumin (BSA), cytochrome C (Cytochrome C), horse myocardium (Myoglobin), egg albumin (Ovalbumin), bovine β-casein (β-casein) and milk The extracted casein (Casein) was dissolved in 1.0mL water respectively, and after denatured by heating, ammonium bicarbonate solution was added to the solution to adjust the pH of the system to about 8, and 25 μg trypsin. At a temperature of 37°C, the enzymatic hydrolysis was terminated after 12 hours, and the enzymatic hydrolysis solution was frozen in a -80°C refrigerator until use.

[0034] (2) Separation and enrichment of standard peptides:

[0035] Make 10 μL a concentration of 5 mg mL -1 The magnetic material dispersion solution was added to 400 μL with a concentration of 5 fmol μL -1 In standard peptide aqueous s...

Embodiment 3

[0044] Embodiment 3 surface modification Fe of C8 alkyl chain 3 o 4 @SiO 2 Magnetic material used for enrichment of enzymatic hydrolyzate on gel

[0045] First, the protein in the human lens was extracted, and 150 mg of the protein was separated by two-dimensional gel electrophoresis, and gel spots with a lower concentration were selected for enzymatic hydrolysis on the gel to obtain about 400 μL of enzymatic hydrolysis solution, which was stored at -20°C for later use. Make 10 μL a concentration of 5 mg mL -1 The magnetic material dispersion solution was added to 200 μL of the above enzymatic hydrolysis solution, and shaken at room temperature for 10 minutes. After removing the supernatant under the action of an external magnetic field, wash it repeatedly with pure water three times; then add 2 μL of 50% (v / v) acetonitrile aqueous solution, and spot 0.5 μL of the mixture containing the enriched peptides and magnetic microspheres onto the target plate After drying, MALDI-T...

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Abstract

The invention belongs to the field of inorganic material and analysis technology, in particular to a magnetic carbon ball superficially modifying C8 alkyl chain, a preparing method and an application thereof. The magnetic carbon ball firstly synthesizes a nano-material of magnetic ferroferric oxide, and then synthesizes an Fe3O4 (at) carbon magnetic microsphere material characterized by a core-shell structure, and further adopts C8 to conduct the surface chemical modification to obtain the magnetic carbon ball. The fixed C1-C8 magnetic nanoparticles, as a micro-absorbent, have a large specific surface area and can be used in enriching peptide among biological samples, and the method is simple and effective. The material has a good practical value and application prospect in the field of proteomics and other fields.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and analysis, and in particular relates to a magnetic carbon sphere with a surface-modified alkyl chain and a preparation method and application thereof. Background technique [0002] It is well known that protein composition is diverse and variable, that is, in different cells of the same species or in the same cell at different times, its protein composition is constantly changing, resulting in extremely complex protein composition. At the same time, due to the post-translational modification of proteins, one mRNA often corresponds to multiple proteins, that is, the number of proteins is far more than the number of genes. In terms of relative abundance, the difference between high-abundance proteins and low-abundance proteins is six orders of magnitude or more; moreover, since proteins cannot be "amplified" like DNA, these have caused many low-abundance proteins to be detected on a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08C07K1/14H01F1/11B22F1/02
Inventor 邓春晖陈和美
Owner FUDAN UNIV
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