Method for preparing protein magnetic blotting nanospheres
A magnetic nanosphere and magnetic nanoparticle technology, applied in chemical instruments and methods, and other chemical processes, can solve the problems of low adsorption capacity, low specific surface area, and easy embedding in them, so as to avoid centrifugation and have wide application prospects , The effect of simplifying the centrifugal separation process
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Embodiment 1
[0057] Example 1: Preparation of hemoglobin magnetic nanoimprinted polymer spheres
[0058] 1. Synthesis of superparamagnetic Fe by co-precipitation method 3 o 4 Preparation of nanoparticles
[0059] Basic reaction equation: Fe 2+ +2Fe 3+ +8OH - = Fe 3 o 4 +4H 2 o
[0060] (1) FeCl 2 4H 2 O (1.72g) and FeCl 3 ·6H 2 O (3.72g) was dissolved in 80mL of secondary water deoxidized by nitrogen, stirred and dissolved, wherein Fe 3+ / Fe 2+ =2:1 (molar ratio);
[0061] (2) Transfer the solution to a 250ml three-necked flask, heat it to 80°C under a nitrogen atmosphere, add 10ml of ammonia water (25%) dropwise, and mechanically stir (at a speed of 800rpm), and react for 30 minutes to obtain black
[0062] colored magnetic fluid;
[0063] (3) After cooling, the mixed solution is divided into two phases by magnetic separation with a magnet, and the supernatant is removed to obtain a black superparamagnetic Fe 3 o 4 Gel, washed twice with water to neutral, vacuum dried to...
Embodiment 2
[0080] Example 2: Preparation of Lysozyme Magnetic Nanoimprinted Polymer Balls
[0081] The template protein was added as lysozyme, and the others were the same as in Example 1 to obtain lysozyme magnetic nanoimprinted polymer balls.
Embodiment 3
[0082] Example 3: Preparation of Serum Albumin Magnetic Nanoimprinted Polymer Balls
[0083] Serum albumin lysozyme was added as the template protein, and the others were the same as in Example 1 to obtain serum albumin magnetic nanoimprinted polymer balls.
[0084] exist figure 1 In the effect figure of the ferroferric oxide magnetic nanoparticles in the shown embodiments 1, 2, and 3, the average particle diameter is 12nm; figure 2 In the effect diagram of the hemoglobin magnetic nanoimprinted polymer prepared in Example 1 shown, the average particle diameter is 100nm; image 3 Shown is the isothermal adsorption curve of the hemoglobin magnetic nanoimprinted polymer microspheres prepared in Example 1 on the template molecule hemoglobin.
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