Plant extract with angiotensin converting enzyme inhibitor function and use thereof

The technology of plant extract and angiotensin, which is applied in the field of preparation of plant extract, can solve the problems of less research on effective components and pharmacological effects of rose, and achieve the effects of rich rose resources, significant antihypertensive effect, and rich source of raw materials.

Inactive Publication Date: 2009-02-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the active ingredients and pharmacological effects of roses so far, and there is no report on extracting ACEI active substances from roses.

Method used

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  • Plant extract with angiotensin converting enzyme inhibitor function and use thereof
  • Plant extract with angiotensin converting enzyme inhibitor function and use thereof
  • Plant extract with angiotensin converting enzyme inhibitor function and use thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of plant extract comprises the following steps:

[0028] 1) Dry roses, fully grind the dried rosebuds into powder (fineness 80-100 mesh), soak and extract with ethanol with a volume ratio of 50-95% for 3 times, each time for 12 hours, and filter and combine the extracts for 3 times;

[0029] 2) The combined extracts were concentrated under reduced pressure to the extract, and then dissolved and extracted with 50% ethanol by volume again for 3 times, each time for 1 hour;

[0030] 3) Combine the above 3 extracts dissolved in 50% ethanol by volume, concentrate, and freeze-dry to obtain a powder, which is the obtained plant extract.

Embodiment 1

[0032] 1.1 Test material

[0033] Plant extracts are prepared by the methods described above. Step 2) The ethanol extract obtained by concentrating under reduced pressure was dissolved in water, ethanol and ethyl acetate respectively, filtered, and the filtrate was set aside (final concentration 20 mg / mL).

[0034] Fresh rabbit lungs were used for the extraction of angiotensin-converting enzyme.

[0035] Hippuryl-histidyl-leucine (HHL) is a product of Sigma Company, and it is a 0.3% (w / v) solution prepared with 50 mmol / L HEPES-NapH8.3 buffer before the test.

[0036] 1.2 Test method

[0037] Angiotensin-converting enzyme (ACE) extraction: Fresh rabbit lungs were washed with pre-cooled potassium phosphate buffer (10 mmol / L, pH 8.3) at 4°C, minced and homogenized. 4°C, 5000g·min -1 Centrifuge for 10min, discard the precipitate, and store the supernatant at 4°C, 40000g min -1 Centrifuge for 40min, collect the supernatant, slowly add ammonium sulfate to make the saturation re...

Embodiment 2

[0047] 2.1 Test material

[0048] The plant extract is extracted by the above method. The lyophilized powder was dissolved in ethanol, filtered, and the filtrate was used for later use (final concentration 20mg / mL).

[0049] Captopril, made a solution with distilled water before the test.

[0050] Wistar male rats, weighing 140-180 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. Every five were placed in metal cages, feeding conditions: room temperature 23°C, artificial lighting 12h / d, free to eat and drink.

[0051] 2.2 Test method

[0052] Establishment of two-kidney-one-clip (2KlC) hypertension model: fasting for 12 hours the night before the operation, intraperitoneal injection of pentobarbital sodium anesthesia after weighing the next morning, making an incision along the midline of the abdomen to open the abdominal cavity, and opening the left kidney The left renal artery was bluntly dissociated near the hilus of the kidney, and ...

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Abstract

The invention relates to a plant extract which has the function of an angiotensin-converting enzyme inhibitor and the application thereof, belonging to the technical field of chemical component extraction of plants. The preparation process is as follows: rose is dried and the dried rosebuds are ground into powder; ethanol with concentration of 50 percent to 95 percent is used for extraction for 3 times; filtrations are carried out for 3 times and the extract is combined; the extract is decompressed and condensed into extractum; then ethanol with concentration of 50 percent is used for extraction for three times and the extract is combined, decompressed, cooled and dried. After pharmacology and pharmacodynamics tests, the analysis on the activity of lowering blood pressure of the rosebud ethanolic extract is carried out; the extract is found to be capable of effectively inhibiting the activity of angiotensin-converting enzyme and obviously lowering the blood pressure of hypertensive rats of two-kidney and one-clip type and being capable of being used for preparing medicines or health care products for curing or preventing hypertension. The preparation method of the extract is simple and practical and the plant extract prepared by the invention has obvious effect of lowering blood pressure and good application safety.

Description

1. Technical field [0001] The invention relates to a preparation method and application of a plant extract, belonging to the technical field of plant chemical component extraction. The extract is extracted and separated from Rosaceae Rosa plant rose, has the function of inhibiting the activity of angiotensin converting enzyme, and is used for treating or preventing hypertension and its complications. 2. Background technology [0002] With the continuous improvement of people's living standards, the average life expectancy is continuously extended, the society is gradually aging, and the prevalence of hypertension is also gradually increasing. According to the statistics of the National Hypertension Research Center, there are 120 million hypertensive patients in my country, with an incidence rate of 10.8%, and its harm is second only to malignant tumors. Therefore, the study of cardiovascular drugs, especially antihypertensive drugs, and the treatment and prevention of hyper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P9/12A61K133/00
Inventor 张炜谢雅晶
Owner NANJING AGRICULTURAL UNIVERSITY
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