Complex phospho-esterase, extraction method and complex phospho-esterase capsule
A technology of compound phosphatase and extraction method, which is applied in the field of capsules, can solve the problems of single dosage form, increased production cost, and low activity of compound phosphatase, and achieve the effects of low preparation cost, resource and energy saving, and convenient storage
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Embodiment 1
[0033] Embodiment 1: extract complex phosphatase from the water extract of barley malt root
[0034] Crush 3kg of malt roots into 80 mesh, add 24L of water at a mass volume ratio of 1:8, extract at a temperature not higher than 20°C for 20 hours, then centrifuge at a speed of 4000 rpm for 25 minutes, and filter out the water phase to obtain Aqueous extract of barley sprout root.
[0035] Take 20 L of the above-mentioned barley sprout root aqueous extract, and at 25°C, while stirring, slowly add 11.22 kg of ammonium sulfate into the barley sprout root aqueous extract, continue stirring for 30 minutes, after sufficient precipitation, place in a centrifuge at room temperature Centrifuge for 25 minutes at 4000 rpm, discard the supernatant.
[0036] At -20°C, use 800ml of acetone-water solution (where the volume ratio of acetone:water is 3:2) to fully wash the phosphodiesterase precipitate, and then centrifuge in a centrifuge at 4°C for 15 minutes at a speed of 4000 rpm , discard...
Embodiment 2
[0038] Embodiment 2: extract complex phosphatase from the water extract of barley malt root
[0039] Take 20 L of the water extract of barley sprout root in Example 1, and slowly add 9.44 kg of ammonium sulfate into the water extract of barley sprout root at 25°C while stirring, and continue stirring for 45 minutes. Centrifuge for 25 minutes in a centrifuge at 4000 rpm, discard the supernatant.
[0040] At -20°C, use 1000ml of acetone-water solution (where the volume ratio of acetone:water is 3:2) to fully wash the phosphodiesterase precipitate, and then centrifuge in a centrifuge at 4°C for 15 minutes at a speed of 4000 rpm , discard the supernatant. Repeat this wash-centrifugation process 4 times.
[0041] Wash the precipitate with 800 ml of pure acetone at -20°C, then centrifuge in a centrifuge at 4°C for 25 minutes at 4000 rpm, and discard the supernatant. Repeat this wash-centrifugation process 2 times. Then the obtained precipitate was air-dried at room temperature a...
Embodiment 3
[0042] Embodiment 3: extract complex phosphatase from the water extract of barley malt root
[0043] Take 20 L of the water extract of barley sprout root in Example 1, and slowly add 4.86 kg of ammonium sulfate into the water extract of barley sprout root at 25°C while stirring, and continue stirring for 50 minutes. Centrifuge in a centrifuge for 25 minutes at a speed of 4000 rpm, discard the supernatant, and obtain 100 g of precipitate.
[0044] At -20°C, use 1000ml of acetone-water solution (where the volume ratio of acetone:water is 7:3) to fully wash the phosphodiesterase precipitate, and then centrifuge in a centrifuge at 4°C for 15 minutes at a speed of 4000 rpm , discard the supernatant. Repeat this wash-centrifugation process 4 times.
[0045] Wash the precipitate with 800 ml of pure acetone at -20°C, then centrifuge in a centrifuge at 4°C for 25 minutes at 4000 rpm, and discard the supernatant. Repeat this wash-centrifugation process 3 times. Then the obtained pre...
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