Separating and extracting method of Bacillus subtilis lipopeptide antibiotic substance

A Bacillus subtilis, extraction method technology, applied in the field of separation and extraction of Bacillus subtilis lipopeptide antibacterial substances, can solve the problems of high equipment requirements, unsuitable for large-scale production, complicated operation, etc. Spectrum antifungal activity, simple and rapid preparation process

Inactive Publication Date: 2009-02-25
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
View PDF0 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art extraction method is complex in operation and requires high equipment requirements, and can be used for

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separating and extracting method of Bacillus subtilis lipopeptide antibiotic substance

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Preparation of crude extract of Bacillus subtilis fermentation broth:

[0016] Bacillus subtilis was inoculated into LB medium and cultured with shaking at 37° C. and 200 rpm for 24 hours to obtain the fermentation broth of Bacillus subtilis. The LB medium: 1 g of peptone, 0.5 g of yeast powder, 1 g of NaCl, 100 mL of water, pH 7.2-7.4.

[0017] Take 50 mL of the above-prepared Bacillus subtilis fermentation broth, centrifuge at 6000 rpm for 10 minutes, take the supernatant, and add 100 mL of acetone to the supernatant, precipitate overnight at 4°C, and collect the supernatant by centrifugation at 12,000 rpm Concentrate to 5mL by rotary evaporation at 60°C and freeze-dry to obtain 200mg of dry matter of the crude fermentation broth, ready for use.

[0018] Weigh 40g of Sephdex G-25 gel, fully swell it, and pack it in a conventional column with a bed volume of 100mL (φ1.7cm×44cm), and prepare the crude extract of the freeze-dried fermentation broth obtained above into a 400m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of biotechnology, in particular to a method for separating and extracting bacillus subtilis lipopeptid antibacterial material. The method more particularly comprises the steps Sephadex G-25 column chromatography is carried out on crude extract of fermentation liquor of the bacillus subtilis, active peak is collected by chromatography, and the Sephadex G-25 column chromatography is carried out on the active peak which is collected by Sephadex G-25 column; the active peak is collected, and the obtained substance is seven lipopeptid antibacterial materials after being detected by mass spectrum. Furthermore, the extracted lipopeptid antibacterial material has good inhibition effect on multiple pathogenic fungis, and has the advantages of stable temperature and pH value, prolease hydrolysis resistance, etc.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for separating and extracting bacillus subtilis lipopeptide antibacterial substances. technical background [0002] Bacillus subtilis can produce a variety of antibacterial substances, and lipopeptide antibacterial substances are one of the most important types. They have strong antibacterial activity. These antibacterial substances are stable in nature and have good resistance to high temperature, acid-base, and protease. Receptivity, and low toxicity, low residue, has potential application value in biological pesticides, feed additives, food additives, antifungal drugs, antiviral drugs, etc., and has become a hot research topic at home and abroad. [0003] Bacillus subtilis lipopeptide antibacterial substances are usually divided into three groups due to their different structures: iturins, surfactin and plipastatin-fengycin family antibacterial substances, iturins are pep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/16C12P21/00C12R1/125
Inventor 李旭张惠文苏振成张成刚
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap