Method for preparing molecular imprinting polymer capable of identifying oxytetracycline and enrofloxacin

A technology of molecular imprinting and enrofloxacin, applied in chemical instruments and methods, organic chemistry, other chemical processes, etc., can solve problems such as the limitation of identification ability, and achieve the effect of improving detection sensitivity and detection efficiency, and promoting the application value.

Inactive Publication Date: 2009-04-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ability of molecularly imprinted polymers prepared by the methods described in these articles to recognize a variety of antibiotics is limited to the same class of antibiotics
In terms of multi-residue analysis of drugs, molecularly imprinted polymers that can handle different classes of antibiotics have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Oxytetracycline 0.1mmol, Enrofloxacin 0.1mmol, NiCl 2 Mix 0.1mmol, MAA 0.2mmol, and EGDMA 2.0mmol, pour into 5ml of methanol and water mixture, the volume ratio of methanol to water is 4:1, after standing at room temperature for 1 hour, add 10mg of initiator AIBN.

[0023] 2. Put the above mixture into an ampoule, and degas it by ultrasonic vibration for 10 minutes. The degassed system was purged with nitrogen for 5 minutes. Quickly seal under nitrogen protection. 60 ℃ water bath insulation reaction for 24 hours. The reaction product is obtained.

[0024] 3. After the reaction is over, the prepared molecularly imprinted polymer is dispersed and washed with ethanol. After three times of sedimentation with acetone, the obtained polymer is dried, and the particle diameter of the polymer is between 0.1-1.5 μm.

[0025] 4. Remove template molecules by Soxhlet extraction for 24 hours. The extraction reagents are methanol, acetic acid and 1.0mol L -1 The mixture of ...

Embodiment 2

[0028] 1. Mix oxytetracycline 0.1mmol, enrofloxacin 0.1mmol, CuSO 4 Mix 0.1mmol, MAA 0.4mmol, and EGDMA 2.0mmol, pour into 5.5ml of methanol-water mixture, wherein the volume ratio of methanol and water is 4:1, and after standing at room temperature for 1 hour, add 10mg of initiator AIBN.

[0029] 2. Put the above mixture into an ampoule, and degas it by ultrasonic vibration for 10 minutes. The degassed system was purged with nitrogen for 5 minutes. Quickly seal under nitrogen protection. 60 ℃ water bath insulation reaction for 24 hours. The reaction product is obtained.

[0030] 3. After the reaction is over, the prepared molecularly imprinted polymer is dispersed and washed with ethanol. After three times of sedimentation with acetone, the obtained polymer is dried, and the particle diameter of the polymer is between 0.1-1.5 μm.

[0031] 4. Remove template molecules by Soxhlet extraction for 24 hours. The extraction reagents are methanol, acetic acid and 1.0mol L -1 ...

Embodiment 3

[0034] 1. Mix oxytetracycline 0.1mmol, enrofloxacin 0.1mmol, FeSO 4 Mix 0.1mmol, MAA 0.8mmol, and EGDMA 2.0mmol, pour 6.0ml of methanol-water mixture, the volume ratio of methanol and water is 4:1, and after standing at room temperature for 1 hour, add 10mg of initiator AIBN.

[0035] 2. Put the above mixture into an ampoule, and degas it by ultrasonic vibration for 10 minutes. The degassed system was purged with nitrogen for 5 minutes. Quickly seal under nitrogen protection. 60 ℃ water bath insulation reaction for 24 hours. get the reaction product.

[0036] 3. After the reaction is over, the prepared molecularly imprinted polymer is dispersed and washed with ethanol. After three times of sedimentation with acetone, the obtained polymer is dried, and the particle diameter of the polymer is between 0.1-1.5 μm.

[0037]4. Remove template molecules by Soxhlet extraction for 24 hours. The extraction reagent is 180ml methanol, acetic acid and 1.0mol L -1 The mixture of HCl...

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PUM

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Abstract

The invention relates to a preparation method of a molecularly imprinted polymer which can distinguish terramycin and enrofloxacin base in the biochemistry engineering technology field. The method has the following steps: terramycin, enrofloxacin base, metallic ions, methacrylic acid and ethylene glycol dimethacrylate are mixed according to the mol ratio of 0.2-1: 0.6-1:1:2-10:20, and then dissolved in a porosity-making agent and added with an evocating agent azodiisobutyronitrile; the mixed liquor is placed in an ampere bottle and charged with nitrogen; the mouth of the ampere bottle is sealed under the protection of nitrogen; the water bath heat preservation reaction is carried out; after the synthetic polymer is stirred and scattered, kernels with the grain diameter of 0.1-1.5 Mum are obtained and subtle kernels are removed; the Soxhlet extraction is carried out; and the polymer which is gotten rid of template molecules is dried and stays the night, and the molecularly imprinted polymer is obtained. The polymer prepared by the method can be applied to the detection of residual terramycin and enrofloxacin base in the high water content environment or organism samples, and the enrichment of trace analytes and the removal of the matrix.

Description

technical field [0001] The invention relates to a method in the technical field of biochemical engineering, in particular to a method for preparing a molecularly imprinted polymer capable of recognizing oxytetracycline and enrofloxacin. Background technique [0002] At present, in order to ensure the health of the people and prevent the unreasonable use of antibiotics, countries all over the world have made strict regulations on the use and residue of antibiotics (such as oxytetracycline and enrofloxacin) and formulated corresponding standards, such as the European Union, Japan, the United States and the Ministry of Agriculture of my country have stipulated different minimum detection limits for enrofloxacin residues in various animal foods and different tissues, for example, the maximum limit in milk should not be higher than 100ppm. Oxytetracycline belongs to tetracycline antibiotics, and enrofloxacin is the third generation of fluoroquinolones (Fluoroquinolones). Both are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/14C08F220/06C08F2/00C08J9/26B01J20/26B01J20/30C07C237/26C07D215/56
Inventor 张大兵瞿国润武爱波
Owner SHANGHAI JIAO TONG UNIV
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