Method for producing nano-fibre bracket material with levorotation polylactic acid as base material

A technology of L-polylactic acid and nanofibers, applied in the direction of scaffolds, fiber types, fiber treatment, etc., can solve the problems of long degradation time, degradation products easily cause tissue inflammation, and cannot be widely used, and achieve simple preparation process and excellent biophase Capacitance and mechanical properties, the effect of excellent tissue compatibility

Inactive Publication Date: 2009-04-08
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned PLLA porous scaffold still has defects such as the degradation time is too long, and the degradation products are easy to cause tissue inflammation, so it cannot be widely used.

Method used

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  • Method for producing nano-fibre bracket material with levorotation polylactic acid as base material
  • Method for producing nano-fibre bracket material with levorotation polylactic acid as base material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of spinning solution: Dissolve L-polylactic acid (PLLA) in a mixed solution of dichloromethane and dimethylformamide (volume ratio = 3: 1), magnetically stir for two hours and centrifuge at 5000 rap / min to obtain By removing the air bubbles in the solution, a uniform and stable spinning solution can be obtained.

[0024] 2. Preparation of nanofibrous membrane: as figure 1 As shown, the above-mentioned L-polylactic acid solution is placed in the driving device 5, in the glass syringe of 5ml (No. For the polylactic acid solution, the flat receiver 1 is used as a collection device for nanofibers, and the distance between the collection device and the needle is kept between 15-20 cm. The prepared L-polylactic acid nano-super ordered fiber membrane was stored at room temperature and dried under vacuum for future use.

[0025] 3. Modification of nanofibrous scaffold materials: the nano-super ordered fiber material obtained in step 2 is modified by low-tempera...

Embodiment 2

[0027] 1. As in step 1 in Example 1.

[0028] 2. Preparation of nanofibrous membrane: as figure 2 As shown, the above-mentioned L-polylactic acid solution was placed in a 5ml glass syringe (No. The high-speed rotation of motor 7, insulated high-speed (wire) drum 6 receiver 1000r / min is used as the collecting device of nanofiber, and the distance between collecting device and needle head remains between 15-20cm. The prepared L-polylactic acid nano-super ordered fiber membrane was stored at room temperature and dried under vacuum for future use.

[0029] 3. Modification of nanofibrous scaffold material: as step 3 in Example 1.

Embodiment 3

[0031] 1. As in step 1 in Example 1.

[0032] 2. Preparation of nanofibrous membrane: as figure 2 As shown, the above-mentioned L-polylactic acid solution was placed in a 5ml glass syringe (No. The high-speed rotation of the motor 7, the insulating high-speed (wire) drum 6 receiver is used as the collecting device of nanofiber at 2000r / min, and the distance between the collecting device and the needle remains between 15-20cm. The prepared L-polylactic acid nano-super ordered fiber membrane was stored at room temperature and dried under vacuum for future use.

[0033] 3. Modification of nanofibrous scaffold material: as step 3 in Example 1.

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Abstract

The invention relates to a method for preparing a nanofiber bracket material using levorotatory polylactic acid as matrix. The method comprises the following steps: dissolving the levorotatory polylactic acid as the matrix in a solution of dichloromethane and dimethyl formamide, and stirring and centrifuging the mixture to obtain an electrostatic spinning solution; placing a polylactic acid solution into a 5 milliliter glass syringe, and applying high voltage on the glass syringe; advancing the levorotatory polylactic acid solution in the glass syringe; preparing the mixed solution into a nanofiber material film through a electrostatic spinning technology; and modifying the nanofiber material film to obtain the nanofiber bracket material of which the fiber diameter is between 50 and 500 nanometers and the fiber porosity is more than 90 percent. The method solves the defects that a PLLA porous bracket still has too long degradation time, and degradation products can cause tissue inflammations easily and the like. The method has the advantages of flexible texture, better water permeability and air permeability, excellent tissue compatibility, controllable biodegradability, and no antigenicity.

Description

technical field [0001] The invention relates to the preparation of a tissue repair material, in particular to a preparation method of a nanofiber scaffold material with L-polylactic acid as a matrix. Background technique [0002] Poly-L-lactic acid (PLLA) belongs to α-polyesters, and the final metabolite of its degradation in vivo is CO 2 and H 2 O, does not accumulate in the body, has almost no toxic and side effects, and has been widely used in the fields of tissue engineering and medicine because of its reliable biological safety, degradability, degradation adjustability, and non-antigenicity. [0003] Before the present invention, the PLLA porous scaffold was prepared by thermal gelation technology, which was similar to the structure of natural interstitial cells, and the in vitro culture experiment of neural stem cells was carried out on the scaffold, and the neural stem cells could differentiate on the scaffold, and can promote neurites growth. Using heat-induced ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61F2/82D01D5/00D06M10/00D06M15/15D06M101/32
Inventor 卢辉俊卫志庆刘长建
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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