Use of ursolic acid and plant extract containing the same
A technology of plant extracts and ursolic acid, which can be used in applications, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as liver and kidney function damage, prevent and treat diabetes and obesity, and reduce sugar intake. Injection, the effect of suppressing the rise of blood sugar level
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Embodiment 1
[0033] Embodiment 1 The preparation of the rosemary extract containing WRY compound and WRY compound
[0034] 1. Preparation of rosemary extract
[0035] Grind 1000 grams of dried rosemary (produced in Jiangxi) leaves and stems into dry powder, soak and extract in petroleum ether twice (45°C, 2h / time); recover petroleum ether; dry residue, 95% ethanol (weight of residue: 95% ethanol weight = 1:10) extract and dry the slag twice (65°C, 2h / time); filter to obtain the alcohol extract; the alcohol extract is directly concentrated under reduced pressure to a dry product to obtain 50 grams, the dry product is Rosemary extract.
[0036] 2. Preparation of WRY compounds
[0037] Take 10 grams of the above-mentioned rosemary extract to isolate the WRY compound, and obtain 500 mg of the pure WRY compound. The content of the WRY compound in the rosemary extract is 5 wt%.
[0038] The separation method of WRY is according to the reference: the preparation method of extracting ursolic ac...
Embodiment 2
[0039] Example 2 Rosemary extract containing WRY compound and WRY compound inhibit test of α-glucosidase activity
[0040] 1. Test principle: The degradation effect of α-glucosidase on α-glucoside is tested by a spectrophotometer, so as to detect the inhibitory effect of the sample on the activity of α-glucosidase.
[0041] 2. Test conditions:
[0042]Instruments: 722-type grating spectrophotometer (manufactured by Shanghai Third Analytical Instrument Factory), electric heating constant temperature water bath (Shanghai Jinghong Experimental Equipment Co., Ltd.), microanalytical balance AE240 (manufactured by Mettler-Toledo, Switzerland), PHB- Type 1 portable PH / ORP meter (manufactured by Shanghai Sanxin Instrument Factory); main reagents: α-glucosidase (Sigma Company of the United States), α-p-nitrophenol glucoside (Sigma Company of the United States), 1,3-butanediol , Sodium Hydroxide, Disodium Hydrogen Phosphate, Potassium Dihydrogen Phosphate (manufactured by Shanghai Reag...
Embodiment 3
[0047] Embodiment 3: The rosemary extract containing WRY compound and WRY compound are to the verification experiment of the rat that inhibits the blood sugar rise caused by oral starch
[0048] 1. Configuration of experimental samples
[0049] Experimental solution 1: 900mg of WRY compound + 900mg of gum arabic + purified water to 90ml.
[0050] Experimental solution 2: 1800 mg of rosemary extract obtained in Example 1 + 900 mg of gum arabic + purified water to 90 ml.
[0051] Control solution: Gum Arabic 900mg + purified water to 90ml.
[0052] Starch paste: 200g of edible starch + 500ml of purified water, stir, heat, and dissolve until a uniformly distributed paste.
[0053] Experimental solution 1 is calculated on the basis of a 60-kg adult, and the recommended dosage per serving is 0.5ml / kg.b.w, with a medium-dose group; experimental solution 2 is calculated on the basis of a 60-kg adult, and the recommended dosage per serving is 0.5ml / kg .b.w, set up a medium-dose gro...
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