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Minitype bionic bracket reactor

A reactor and bionic technology, applied in the fields of clinical medicine, biomedicine and regenerative medicine, can solve unsatisfactory problems, achieve the effects of promoting growth and metabolism, maintaining long-term biological functions, and simplifying structure and operation

Inactive Publication Date: 2009-05-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These requirements are obviously unable to meet the large hollow fiber reactors used in industrial production

Method used

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  • Minitype bionic bracket reactor
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  • Minitype bionic bracket reactor

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0026] Specific Example 1 (comparison of culturing rat hepatocytes in micro-bionic scaffold reactor and monolayer adherent method)

[0027] The oxygen-transmitting silica gel envelope 1 is 15 mm long, and the material of the three-dimensional porous support 2 is PLGA, and the molecular weight of PLGA is 70,000 units. The porous scaffold 2 has a diameter of 9mm, a thickness of 3mm, and a pore diameter of 100-200nm. There are 6-8 specific lumens with a pore diameter of 400nm. Four porous scaffolds are stacked together. The diameter of the seal 3 of the oxygen transmission silicone tube is 2 mm. The specific lumen is laser-drilled in a three-dimensional scaffold without other materials, and is mainly used to provide endothelial cell growth and induce microangiogenesis.

[0028] Rat primary hepatocytes obtained by routine isolation with an activity higher than 90% were treated with 10 7 The cells / ml density was inoculated into the medium of DMEM containing 15% FBS (purchased fro...

specific Embodiment 2

[0036] Specific Example 2 (Comparison of Miniature Biomimetic Scaffold Reactor and Monolayer Adhesive Method Differentiation of Rat Bone Marrow Mesenchymal Stem Cells (BMSCs) to Hepatocytes)

[0037] The oxygen-transmitting silica gel envelope 1 is 15 mm long, and the material of the three-dimensional porous support 2 is PLGA, and the molecular weight of PLGA is 70,000 units. The porous scaffold 2 has a diameter of 9mm, a thickness of 3mm, and a pore diameter of 100-200nm. There are 6-8 specific lumens with a pore diameter of 400nm. Four porous scaffolds are stacked together. The diameter of the seal 3 of the oxygen transmission silicone tube is 2 mm.

[0038] Rat BMSCs routinely isolated to an activity higher than 90% by 10 7 The cells / ml density was inoculated into the IMDM (purchased from Invitrogen) medium containing hepatocyte growth factor, and the cells were injected into the PLGA three-dimensional porous scaffold with a syringe, and the amount of cells per scaffold wa...

specific Embodiment 3

[0044] Specific embodiment 3 (miniature biomimetic stent reactor is used for the research of drug metabolism)

[0045] The envelope 1 made of oxygen-transmitting silica gel material is 15 mm long, and the material of the three-dimensional porous support 2 is PLGA, and the molecular weight of PLGA is 70,000 units. The porous scaffold 2 has a diameter of 9mm, a thickness of 3mm, and a pore diameter of 100-200nm. There are 6-8 specific lumens with a pore diameter of 400nm. Four porous scaffolds are stacked together. The diameter of the seal 3 of the oxygen transmission silicone tube is 2mm.

[0046] Rat hepatocytes with activity higher than 90% were isolated by routine laboratory by 10 6 The cells / ml density was inoculated into the medium of DMEM containing 15% FBS (purchased from Invitrogen Company), and injected into the PLGA three-dimensional porous scaffold in the micro-bionic scaffold reactor using a syringe, and the cell amount of each scaffold was 10 6 The cells were cen...

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Abstract

The invention discloses a minitype bionic bracket reactor which comprises an oxygen-transfer silica gel wrapper, more than one three-dimensional porous bracket and an oxygen-transfer silica gel sealing port; the three-dimensional porous bracket is arranged in a polyglycolic acid or polylacti-co-glycolic acid wrapper; and two ends of the oxygen-transfer silica gel wrapper is sealed by an oxygen-transfer silica gel pipe. The minitype bionic bracket reactor has the advantages that the bracket greatly simplifies the structure and operation of a common biological reactor, farthest stimulates an in-vitro microenvironment of a cultured cell, obviously promotes growth and metabolism of the cultured cell, maintains long-term biological function of the cultured cell, can be widely applied to various cell cultivations, in particular to a primary cell, and is also very suitable for research of drug metabolism and filtration.

Description

technical field [0001] The invention belongs to the fields of clinical medicine, biomedicine and regenerative medicine, relates to tissue engineering and artificial organ technology, in particular to a miniature bionic bracket reactor. Background technique [0002] The current drug efficacy and pharmacology research is based on animal models, in vitro primary cell models, and immortalized cell line models. Animal models have long experimental cycles, low efficiency, large individual differences that lead to large error in results, and are not conducive to animal protection. defect. In addition, species differences are also the Achilles' heel of animal models. Although immortalized cell lines have the advantages of simple and stable culture, high efficiency and low cost, compared with primary cultured cells, immortalized cell lines are quite different in structure and function. neoplastic potential. [0003] Recently, the use of primary cell models for in vitro drug resear...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 李兰娟李君
Owner ZHEJIANG UNIV