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Production method of N-acetyl-L-glutamine

A technology of glutamine and production method, applied in the production field of L-type amino acid N-acetylation reaction, can solve problems such as large-scale production without manufacturers, and achieve the effects of simple process, wide source of raw materials, and continuous production

Inactive Publication Date: 2009-07-01
NINGBO ZHENHAI HAIDE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

N-acetyl-L-glutamine is also a medicinal amino acid, and there is no large-scale production in China.

Method used

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  • Production method of N-acetyl-L-glutamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Mix L-glutamine and water at a weight ratio of 1:5, add 0.03% sodium sulfite by weight of raw materials, and simultaneously add acetic anhydride dropwise to control pH7, acylate at 78°C for 1 hour, and add 6N hydrochloric acid dropwise after the acylation is complete Adjust the pH to 0.5, concentrate until crystals are precipitated, stop the concentration, stir, freeze and crystallize to 2°C, centrifuge, and dry to obtain the crude product. Dissolve the crude product in deionized water with a mass ratio of 3 times, add 3% activated carbon for decolorization, and then obtain the finished product after filtration, concentration, crystallization, centrifugation and drying. The yield can reach 92% and the purity is 99.5%.

Embodiment 2

[0027] Mix L-glutamine and water at a weight ratio of 1:1, add 0.01% sodium bisulfite by weight of raw materials, and at the same time add 40% sodium hydroxide dropwise to control pH 12, acylate at 40°C for 1.5h, until acylation After completion, add 6N hydrochloric acid dropwise to adjust the pH to 3.0, concentrate until crystals are precipitated, stop the concentration, stir, freeze and crystallize to 10°C, centrifuge, and dry to obtain a crude product. Dissolve the crude product in deionized water with a mass ratio of 7 times, add 5% activated carbon for decolorization, and then obtain the finished product after filtration, concentration, crystallization, centrifugation and drying. The yield can reach 95% and the purity is 99.25%.

Embodiment 3

[0029] Mix L-glutamine and water at a weight ratio of 1:10, add 0.05% sodium thiosulfate by weight of raw materials, and at the same time add 35% sodium hydroxide dropwise to control pH9, acylate at 5°C for 3 hours until the acylation is complete Finally, add 6N hydrochloric acid dropwise to adjust the pH to 2.0, concentrate until crystals are precipitated, stop the concentration, stir and freeze to crystallize to 5°C, centrifuge and dry to obtain the crude product. Dissolve the crude product in deionized water with a mass ratio of 5 times, add 4% activated carbon for decolorization, and then obtain the finished product after filtration, concentration, crystallization, centrifugation and drying. The yield can reach 92% and the purity is 99.8%.

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Abstract

The invention provides a method for producing N-acetyl-L-glutamine, which comprises: dissolving L-glutamine in water, adding proper amount of catalyst, slowly adding acetic anhydride and a sodium hydroxide solution, adjusting the pH value to be between 7 and 12, and controlling the temperature to be between 5 and 80 DEG C, reacting for 1 to 3 hours, dropwise adding 6N hydrochloric acid to adjust the pH value to be between 0.5 and 3.0 after complete acylation, performing stirring, freezing and crystallization after concentration and de-watering until the temperature is between 0 and 10 DEG C, and performing centrifugation and drying to obtain coarse products; and dissolving the coarse products in deionized water of which the mass is 3 to 7 times of the coarse products, adding active carbon for decolorization, and obtaining finished products after filtration, concentration, crystallization, centrifugation and drying. Amino acid produced by the method has various pharmacological activities and clinic functions, has wide potential market, not only can greatly improve the production level of the amino acid in China but also has significance in improving the health level of people, and has high social benefit and economic benefit.

Description

technical field [0001] The invention relates to a synthesis method for derivatizing N-acetyl-L-glutamine from L-glutamine by a chemical method, and belongs to the technical field of production of L-type amino acid N-acetylation reaction. Background technique [0002] Amino acid is one of the many biologically active macromolecules that build biological organisms, and is the basic material for building cells and repairing tissues. Amino acids are used by the human body to manufacture antibody proteins to fight against bacterial and viral infections, to manufacture hemoglobin to transport oxygen, to manufacture enzymes and hormones to maintain and regulate metabolism; amino acids are the main substance for the production of sperm and egg cells, and are indispensable for the synthesis of nerve media Prerequisite substances; amino acids can provide energy for body and brain activities, and amino acids are the essence of all life. Therefore, as an extremely important material un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/22C07C231/14
Inventor 王雷孙军缪铭周玉平张姝张勇吴培立黎法国潘庆伟
Owner NINGBO ZHENHAI HAIDE BIOCHEM
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