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Method for preparing ellagic acid from pomegranate bark by enzyme process

A technology of ellagic acid and pomegranate peel, which is applied in the fields of medicine, chemical industry and food, can solve the problems of cumbersome and complicated preparation process, difficult separation and purification, difficulty in ellagic acid, etc., and achieve simple process, saving consumption and easy treatment Effect

Inactive Publication Date: 2009-07-15
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the preparation process of gallic acid (ester) is loaded down with trivial details and complicated, and enzyme also needs refined purification, and also has a kind of unknown quinone substance in the product, and its ratio with ellagic acid is 1: 2.4, has brought separation and purification to product Certain difficulties, currently seldom use this method for the production of ellagic acid
[0008] Microbial production of ellagic acid uses rubber tannin as raw material to produce ellagic acid during the fermentation process. Since ellagic acid is insoluble in water, a large amount of mycelium is produced during the fermentation process, and the reaction time of this method is long , which brings great difficulties to the subsequent separation and purification
It is not easy to obtain high-purity ellagic acid

Method used

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  • Method for preparing ellagic acid from pomegranate bark by enzyme process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A Extraction of ellagitannins:

[0026] After crushing 80 kg of dried pomegranate peels, add them to 600 L of 40% ethanol aqueous solution, conduct ultrasonic treatment for 25 minutes under the action of ultrasonic waves with a power of 80W, take out and collect the filtrate, and repeat the above ultrasonic treatment for the remaining residue Second, the obtained ethanol aqueous solution of ellagitannin was concentrated to recover ethanol to obtain 240 L of concentrated ellagitannin solution.

[0027] Fermentation culture of B tannin:

[0028] The composition and content of the fermented seed medium are: NaNO 3 2g / l, K 2 HPO 4 1g / l, MgSO 4 ·7H 2 O 0.6g / l, KCl 0.6g / l, FeSO 4 0.01g / l, sucrose 30g / l, and the rest is water. After sterilization, add Aspergillus niger 3.316 immediately, at 30 ℃, 165rpm Shaker culture 24 hours, move in the enzyme production medium with 5% inoculum size, the composition and mass content of enzyme production medium: ellagitation Tanni...

Embodiment 2

[0033] A Extraction of ellagitannins:

[0034]After pulverizing 80 kg of dried pomegranate peels, add them to 600 L of ethanol aqueous solution with a volume concentration of 60%, and conduct ultrasonic treatment for 30 minutes under the action of ultrasonic waves with a power of 100 W, take out and collect the filtrate, and repeat the above ultrasonic treatment twice for the remaining residue , to obtain an ethanol aqueous solution of ellagitannin, and after concentration and recovery of ethanol, 200 L of ellagitannin concentrated solution was obtained.

[0035] Fermentation culture of B tannin:

[0036] The composition and content of the fermented seed culture medium are: NaNO 3 1.5g / l, K 2 HPO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, KCl 0.5g / l, FeSO 4 0.01g / l, sucrose 25g / l, and the rest is water. After sterilization, add Aspergillus niger 3.316 immediately, at 28 ℃, shaker culture at 165rpm for 22 hours, move to the enzyme-producing medium with 5% inoculum, the composit...

Embodiment 3

[0042] A Extraction of ellagitannins:

[0043] After crushing 80 kg of dried pomegranate peels, add them to 600 L of ethanol aqueous solution with a volume concentration of 50%, and perform ultrasonic treatment for 40 minutes under the action of ultrasonic waves with a power of 90W, take out and collect the filtrate, and repeat the above ultrasonic treatment once for the remaining residue , After the obtained ethanol aqueous solution of tannic acid was concentrated and recovered ethanol, 200 L of ellagitannin concentrate was obtained.

[0044] Fermentation culture of B tannin:

[0045] The composition and content of the fermented seed medium are: NaNO 3 2.5g / l, K 2 HPO 4 1.5g / l, MgSO 4 ·7H 2 O 0.8g / l, KCL 0.8g / l, FeSO 4 0.015g / l, 35g / l sucrose, and the rest is water. After sterilizing, add Aspergillus niger 3.316 immediately, at 25 DEG C, 165rpm down shaker culture 24 hours, move in the enzyme production medium with 5% inoculum size, the composition and content of enz...

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Abstract

The invention relates to a method of using pomegranate rind as raw material to prepare ellagic acid by enzymatic method, belonging to the food, medical and chemical fields. The method uses the pomegranate rinds which are numerously discarded by people in China as raw materials to extract ellagitannin. The characteristics of high catalysis efficiency, strong selectivity, moderate processing conditions, and the like of enzyme are used to obtain tannase containing degraded ellagitannin through fermentation. The enzymatic reaction between the tannase and the ellagitannin happens to generate the ellagic acid. After the solvent crystallization purification, the medical ellagic acid with dear price and high purity is obtained. The method can lower the cost of the raw material, reuse wastes, improve the yield and the purity of the ellagic acid and can be applied to the large scale production.

Description

Technical field: [0001] The invention relates to a method for enzymatically preparing ellagic acid from pomegranate peel. It belongs to the fields of food, medicine and chemical industry. Background technique: [0002] Ellagic acid (2,3,7,82tet rahydroxybenzopyrano[5,4,3cde]benzopyran-5,10-dione), is a natural polyphenol component widely present in various soft fruits, nuts and other plant tissues , which is a dimeric derivative of gallic acid, is a polyphenolic dilactone. It can not only exist in free form, but more in condensed form (such as ellagitannin, glycoside, etc.) in nature. Pure ellagic acid is a yellow needle-like crystal with a melting point (pyridine) greater than 360°C, slightly soluble in water and alcohol, soluble in alkali and pyridine, and insoluble in ether. Ellagic acid formula C 14 h 6 o 8 , the molecular weight is 302, and its structure is as follows: [0003] [0004] In the past ten years, due to the discovery of the anti-mutation and anti-...

Claims

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Application Information

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IPC IPC(8): C12P17/18
Inventor 袁其朋程艳张倩
Owner BEIJING UNIV OF CHEM TECH
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