Method for extracting and separating L-isoleucine by membrane separation and plant chromatography separation

Abstract

The invention provides a method for extraction-separating an L-isoleucine by the combination of membrane separation and industrial chromatography separation, and belongs to the field of biochemical engineering. The method consists of the following steps: (1), removing thalli from a fermentation liquor; (2), separating inorganic salts and other amino acids in high efficiency by the industrial chromatography; (3), removing impurities and pigments from the fermentation liquor; and 4, condensing and crystallizing the fermentation liquor, and the like. The method has the characteristics of mild reaction conditions, easy operation, less separation steps, good selectivity, clean production and the like, and overcomes the defects of low yield, large discharge capacity of waste water and high production intensity existing in the prior art and obviously improves the yield and quality of the L-isoleucine.

Description

technical field [0001] The invention relates to a method for extracting and separating L-isoleucine, in particular to a method for separating and extracting L-isoleucine from a fermentation broth by using a combined technology of membrane separation and industrial chromatographic separation. It belongs to the field of biochemical industry. Background technique [0002] As one of branched-chain amino acids, L-isoleucine is an essential amino acid for the human body and has various physiological functions. Because of its special structure and function, it occupies a particularly important position in the metabolism of human life. It is mainly used to prepare compound amino acid infusions, synthetic polypeptide drugs and food antioxidants, etc., especially in medical research and treatment, and is increasingly valued. . It is used in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic defects...

AI Technical Summary

Problems solved by technology

The general process of this process is as follows: the fermentation broth is filtered to remove the bacteria, and the separation and extraction of L-isoleucine from the fermentation broth using this process has the following problems: one is to use the method of filtration to remove the bacteria. Second, the color value of the L-isoleucine fermentation liquid is relatively high, and the decolorization of ordinary activated carbon is not complete and the amount is large, which affects the product quality and yield; the third is that there are many impurities in the L-isoleucine fermentation liquid , high content, direct use of ion exchange method, serious resin pollution, easy to cause damage to the resin structure and loss of regeneration ability, increased operating costs, and high content of ionic substances will quickly saturate the resin, increase the number of resin regeneration, resulting in Large amount of sewage discharge, etc.

Membrane separation technology has been used in the separation and purification of fermentation products. There have been successful precedents, but there is no report on the separation and extraction of L-isoleucine from fermentation broth using membrane separation and industrial chromatographic separation technology.