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Alcohol fermentation anaerobic high temperature bacterium culture medium, preparation and use thereof

A technology of ethanol fermentation and culture medium, which is applied in the biological field and can solve the problem of no bean curd residue

Inactive Publication Date: 2009-08-05
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of bean curd residue as a medium raw material in the existing ethanol fermentation high temperature anaerobic culture technology

Method used

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  • Alcohol fermentation anaerobic high temperature bacterium culture medium, preparation and use thereof
  • Alcohol fermentation anaerobic high temperature bacterium culture medium, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The cultivation effect of bean curd residue as a nutrient substance:

[0025] Preparation of bean curd dregs nutrient stock solution: weigh 20 g bean curd dregs (wet weight), add 80 ml of pure water, stir evenly, adjust the pH value to 7 with an appropriate amount of NaOH solution, and use it as a bean curd dregs nutrient stock solution for later use. The bean curd residue used in this test is a raw material without any processing or treatment, and its moisture content is 75%.

[0026] The preparation of the basal medium of ethanol fermenting high-temperature anaerobic bacteria: the following components are dissolved in 1 liter of pure water, and the pH value is adjusted to 7 with NaOH solution, which is used as the basal medium solution of the high-temperature bacteria culture test for subsequent use.

[0027] Medium components: peptone (peptone): 10g; yeast extract (Yeast extract): 0g; microcrystalline cellulose (Avicel) or glucose (Glucose): 5g; magnesium chloride he...

Embodiment 2

[0035] The method used is the same as in Example 1, except that the content of each component in every liter of medium is: peptone: 5g; microcrystalline cellulose or glucose 50g; magnesium chloride hexahydrate 2g; calcium chloride dihydrate 0.3g; hexahydrate 2.5 mg of ferrous sulfate; 3.0 g of potassium dihydrogen phosphate; 6.0 g of dipotassium hydrogen phosphate; 1.0 g of cysteine ​​hydrochloride; 2.0 mg of resazurin; The culture medium with a concentration of 4.0% by weight of slag.

Embodiment 3

[0037] The method used is the same as in Example 1, except that the content of each component in every liter of medium is: peptone: 10g; microcrystalline cellulose or glucose 100g; magnesium chloride hexahydrate 1g; calcium chloride dihydrate 0.15g; 1.25 mg of ferrous sulfate; 0.75 g of potassium dihydrogen phosphate; 5.0 g of dipotassium hydrogen phosphate; 0.5 g of cysteine ​​hydrochloride; 1.0 mg of resazurin; Slag 3.0% weight ratio concentration of medium.

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Abstract

The invention discloses a microorganism culture medium by using soybean curb residue as culture medium nutritive materials, which provides an alcoholic fermentation high-temperature anaerobic organism culture medium by using soybean curb residue as culture medium nutritive materials, and a preparation method thereof, and the application in alcoholic production and alcoholic fermentation anaerobic organism culture for soybean curb residue and the culture medium thereof. When the weight ratio concentration of added soybean curb residue of liquid water content of 75 percent is not less than 0.5 percent in the culture medium, culture medium nutritive materials using peptone and yeast extract in the existing culture technology can be replaced so as to reach the effects of promoting the growth and reproduction of high-temperature anaerobic organisms and improving alcoholic fermentation and metabolization. The invention provides a low-cost culture medium suitable for the microorganisms, and a technology applying the microorganism culture method of the culture medium and alcoholic production.

Description

Technical field: [0001] The present invention relates to the field of biotechnology, in particular to a microbial culture medium that uses bean curd residue or a substance similar to the components of bean curd residue as nutrients for ethanol-fermented anaerobic high-temperature bacteria culture medium, and a production method, a culture method, and a microbial culture medium utilizing the above-mentioned method. Ethanol fermentation technology, application of bean curd residue and its containing medium in the production of ethanol and the cultivation of ethanol-fermenting anaerobic bacteria. Background technique: [0002] Microbial culture medium is a group of artificially prepared nutrients required for the growth and reproduction of microorganisms. It must contain basic nutrients such as water, carbon sources, nitrogen sources and inorganic salts necessary for microbial life activities. Partial ethanol fermentation high temperature anaerobic bacteria, such as Clostridium...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/06C12R1/145C12R1/01
CPCY02E50/17Y02E50/10
Inventor 伊日布斯尚淑梅李昆志陈丽梅赵立峰
Owner KUNMING UNIV OF SCI & TECH
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