Method for preparing anti-A type botulinus toxin immunoglobulin antibody

A botulinum toxin type A, immunoglobulin technology, applied in the direction of antibacterial immunoglobulins, microorganism-based methods, biochemical equipment and methods, etc. Neutralizing activity and other issues, to achieve high purity, good immune effect, good stability
CN101497909AActive Publication Date: 2009-08-05INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
Publication Date
2009-08-05

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Abstract

The invention discloses a method for preparing an anti-type A botulinum neurotoxin immunoglobulin antibody, which comprises the steps of preparing and obtaining the anti-type A botulinum neurotoxin immunoglobulin antibody through taking type A botulinum neurotoxin recombinant protein rAHc as immunogen to immunize animals. The method for preparing the anti-type A botulinum neurotoxin immunoglobulin antibody eliminates a Fc segment in the antibody which can cause side effect and obtains the horse anti-type A botulinum neurotoxin immunoglobulin F(ab')2 antibody; after blood serum obtained by pentalogy hyper-immune and hexalogy hyper-immune are mixed, the content (purity) of F(ab')2 of the antibody can achieve 80.2 percent in a semi-finished product obtained through further purification; the titer of the antibody can achieve 8000 IU / ml; the antibody has good specificity and sensitivity to type A botulinum neurotoxin; and the purity and the specific activity of the antibody are higher than the antitoxin prepared from the traditional toxin immunity horses. Moreover, the invention has the good stability and safety.
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Description

technical field

[0001] The invention relates to a method for preparing anti-botulinum toxin immunoglobulin antibody. Background technique

[0002] Botulinum toxin (BoNT) is currently the most virulent protein known, and 1.0g of botulinum toxin crystals is enough to kill 1 million people. It is produced by Clostridium botulinum in an anaerobic environment. There are 7 serotypes (A-G) of the exotoxins produced. Among them, A and B types of botulinum toxin are the common types that cause human poisoning, E and F types occasionally cause food poisoning epidemics, and C and D types only cause animal and poultry poisoning. Poisoning, there are fewer reports of poisoning caused by G type. Clinical observations have shown that botulinum toxin type A can cause more severe intoxication symptoms and lead to higher mortality than other types. Botulinum toxin poisoning has a small incidence rate in the crowd, but its toxicity is high, the fatality rate is high, and it is extremely harm...

Claims

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