Colloid selenium test paper for semi-quantitative determination of urinary trace albumin

A urine microalbumin and semi-quantitative detection technology, which is applied in biological testing, material inspection products, and analysis through chemical reactions of materials, can solve problems such as unsuitable for on-site detection, long RIA determination process, and environmental pollution. To achieve the effect of convenient home testing and on-site testing, real-time monitoring and tracking, and simple testing procedures
CN101504417BActive Publication Date: 2013-08-28LANZHOU UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU UNIVERSITY
Publication Date
2013-08-28

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Abstract

The invention provides a test paper for semi-quantitatively detecting colloid selenium in microdose albumin in urine. The test paper can be applied in the fields of early diagnosis of diabetic nephropathy, hypertensive nephropathy and preeclampsia and injury of kidney caused by various poisonous substances. A plastic bottom plate of the test paper is orderly glued with a sampling liquid absorption part, a colloid selenium labeling part, a detection reaction part and a water absorption part, wherein the colloid selenium labeling part is a single-cloning antibody 1 which is coated by a glass fiber membrane, or acetic acid fiber membrane, or nylon membrane and labeled by the colloid selenium; the detection reaction part is a nitrocellulose membrane which is provided with two detection bands and quality control bands, T1 is a single-cloning antibody 2 which is precisely quantitative, and T2 is the single-cloning antibody 2 with a certain concentration; and the quality control bands are coated by antiplague IgG. The invention provides a method for semi-quantitatively detecting the microdose albumin in a urine sample conveniently, visually and quickly.
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Description

1. Technical field

[0001] The invention is a colloidal selenium test paper for semi-quantitative detection of urinary microalbumin, through which the test paper can directly and simply determine whether the albumin content in urine is higher than the normal level, and can be applied to early auxiliary clinical diagnosis of diabetic nephropathy. It belongs to the category of in vitro detection equipment for diseases. 2. Background technology

[0002] Diabetic nephropathy is a chronic kidney complication of diabetes. Diabetic nephropathy generally refers to diabetic glomerulosclerosis, and its clinical manifestations are proteinuria, edema, hypertension or azotemia, and there are symptoms of increased fasting blood sugar or diabetes. There is no obvious regional difference in this disease, and both type I and type II diabetes can be caused. According to statistics, about 50% of diabetic patients with a disease course of 10-20 years, regardless of age, develop clinical kidney...

Claims

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