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Extract of smilax glabra saponins and flavonoid and preparation method thereof

A technology of Smilax saponins and flavonoids, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of being unable to adapt to the requirements of the industrialization of traditional Chinese medicine, unable to produce in large quantities, and unsatisfactory effects, etc., to achieve improved operability, Easy to filter, cost-saving effect

Active Publication Date: 2009-09-09
湖南湘泉药业股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of Smilax smilax extract, the paper "Technology Research on Extracting Total Flavonoids from Smilax Smilax" in "Plant Research" 2006 Volume 26 No. 3 discloses a method for extracting total flavonoids from Smilax Smilax, but the research level of this paper is It is relatively shallow, and only the influence of factors such as temperature and ethanol concentration on the extraction rate has been discussed, and the yield is not high; the patent ("Smilax smilax total glycosides extract and its preparation method and use" application number: 200510095149.3) discloses An extract of total glycosides of Smilax cocos and its preparation method and application, but this method only studies the extract of total glycosides, the yield is low, and it cannot meet the requirements of the industrialization of traditional Chinese medicine
"Study on the Enrichment and Purification of Total Flavonoids of Smilax Smilaxe by Macroporous Adsorption Resin" in "Pharmacy Today" 2008 Volume 18, Issue 3 and "Separation and Purification of Total Flavonoids from Smilax Smilax Smilax" in "Chinese Journal of Traditional Chinese Medicine" 2008 Volume 33 10th Issue 》Respectively disclosed a method for separating and purifying total flavonoids from Smilax smilax alcoholic extract, but these two methods have the problem of high cost and cannot be produced in large quantities
[0005] On the other hand, enzyme preparations have been used in plant extraction and traditional Chinese medicine (such as ginkgo, ginseng, etc.), and have played an important role in the industrialization of traditional Chinese medicine, but most of them use a single enzyme preparation, and the effect is not satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Vacuum dry the raw medicine of Smilax glabra to a moisture content of 5.5%, a drying temperature of 50 degrees, crush to 10 mesh, add 3 times the amount of enzymatic hydrolysis solution and enzymatically hydrolyze at 35 degrees for 9.5 hours. The preparation method of the enzymolysis solution is: dissolving 1.5 g of plant extract enzyme (enzyme activity of 19000 units) in 500 ml of buffer solution with a pH of 4.2. The plant extract enzyme composition is 30% cellulase, 40% pectinase, 10% protease, and 20% xylanase.

[0015] 2. Perform reflux extraction with 5 times the weight of Smilax glabra and 60% concentration of ethanol for 3 hours, repeat three times, combine the extracts, concentrate into a paste, degrease petroleum ether and extract with chloroform twice, then combine the extracts and concentrate And vacuum drying to obtain flavonoid extract; then extract twice with ethyl acetate and combine the extracts, concentrate and vacuum dry to obtain saponin extract.

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Embodiment 2

[0018] 1. Vacuum dry Smilax glabra raw medicinal materials to a moisture content of 7%, a drying temperature of 55 degrees, pulverize to 18 mesh, add 8 times the amount of enzymatic hydrolysis solution and enzymatically hydrolyze at 45 degrees for 4.5 hours. The preparation method of the enzymolysis solution is as follows: dissolving 8 grams of plant extract enzymes (enzyme activity of 21000 units) in 500 ml of buffer solution with a pH of 5.0. The plant extract enzyme composition is 47% cellulase, 21% pectinase, 8% protease, and 24% xylanase.

[0019] 2. Reflux extraction with 8 times the weight of Smilax glabra and 85% concentration of ethanol for 2.5 hours, repeat three times, combine the extracts, concentrate into a paste, degrease petroleum ether and extract with chloroform twice, then combine the extracts and concentrate And vacuum drying to obtain flavonoid extract; then extract twice with ethyl acetate and combine the extracts, concentrate and vacuum dry to obtain saponin ...

Embodiment 3

[0022] 1. Vacuum dry Smilax glabra raw medicinal materials to a moisture content of 9%, a drying temperature of 65 degrees, pulverize to 11 mesh, add 10 times the amount of enzymatic hydrolysis solution to enzymolysis at a temperature of 48 degrees for 5 hours. The preparation method of the enzymolysis solution is as follows: dissolve 3 grams of plant extract enzymes (with an enzyme activity of 30,000 units) in 500 ml of a buffer with a pH of 7. The plant extract enzyme composition is 48% cellulase, 25% pectinase, 17% protease, and 10% xylanase.

[0023] 2. Use 5 times the weight of Smilax glabra, with a concentration of 78% ethanol, to perform reflux extraction for 3.6 hours with the aid of ultrasound. Repeat three times, combine the extracts, concentrate them into a paste, degrease petroleum ether and extract twice with chloroform and combine The extract is concentrated and vacuum dried to obtain flavonoid extract; then it is extracted twice with ethyl acetate and the extracts a...

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Abstract

The invention discloses an extract of smilax glabra saponins and flavonoid and a preparation method thereof. The preparation method comprises the steps of (1) smilax glabra medical materials are dried in vacuum, crushed and hydrolyzed by enzyme; and (2) smilax glabra saponins and flavonoid compound are extracted with ethanol under the auxiliary function of ultrasonic in inverse flow and condensed; the condensed compound is degreased with petroleum ether and extracted with chloroform and ethyl acetate; after the solvent is recovered, the solvent is dried in vacuum to obtain the extract of smilax glabra saponins and flavonoid which contains 30-80 percent of flavonoid compound. The obtained smilax glabra saponins extract contains 20-70 percent of saponins compound. The method utilizes composite plant extracting enzyme to pretreat the smilax glabra medical materials and extract the smilax glabra saponins and flavonoid compound with ultrasonic auxiliary extract technology, has high utilization degree of the medical materials and high yield of product, consumes low energy and is convenient to operate. The extract of smilax glabra saponins and flavonoid is widely applied to the field of medical materials, midbody and health-care food and has wide market foreground.

Description

Technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of extracting saponin and flavonoids from Smilax glabra. Background technique [0002] Smilax glabra is the dried rhizome of Liliaceae Smilax glbra Roxb., but the term Smilax glabra in the present invention also includes the dried rhizome of Smilax glabra, which is a commonly used Chinese herbal medicine, distributed in the Yangtze River basin. South and other places, taste sweet and flat. Smilax glabra and its extracts have the effects of dehumidification, detoxification, and tonic joints, and are mainly used for damp-heat gonorrhea, carbuncle, scabies and the limbs and muscles pain caused by mercury poisoning. [0003] The main active ingredients of Smilax glabra and its extracts are saponins and flavonoids. Therefore, the development of extraction techniques for smilax saponins and flavonoids will improve the utilization of Smila...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P29/00A61P19/02A61P17/00A61K125/00
Inventor 胡江宇谢新平谢勇朱晓妮王锡炳
Owner 湖南湘泉药业股份有限公司
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