Method for comprehensively extracting and purifying oxidation-resistant active ingredient in sugarcane shoots or slag

Abstract

The invention discloses a method for comprehensively extracting and purifying general flavone and anthocyanin in sugarcane toppers or slag. The method comprises the steps of firstly extracting the sugarcane toppers or slag by an organic solvent, concentrating the extract and passing the extract through a chromatographic column filled with a macro-porous adsorptive resin, removing water-soluble impurities such as glucide by a gradient elution method, respectively collecting the ethanol eluent with a high concentration and the ethanol eluent with a low concentration to obtain two sites of the general flavone and anthocyanin, and respectively enriching and purifying the sites of the general flavone and anthocyanin by an acid and alkali treatment method and a solvent extraction method so as to obtain the general flavone and anthocyanin. Compared with the reported methods for extraction and separation of similar ingredients, the method of the invention can not only completely extract two main oxidation-resistant ingredients in the sugarcane toppers or slag, also the reagents consumed in the technological process can be recycled. The method has low production cost, high purity and mature technology, and is easily controlled and in favour of industrial production.

Description

technical field [0001] The invention belongs to the technical field of methods for comprehensively extracting, separating and purifying main antioxidant active ingredients in sugarcane tip or bagasse, and specifically relates to a method for comprehensively extracting and purifying main antioxidant active ingredients in sugarcane tip or bagasse—total flavonoids and anthocyanins . Background technique [0002] Sugarcane (Saccharum officenarum L.) is a plant of the genus Saccharum in the Gramineae family. It is native to tropical and subtropical regions. It has a high light saturation point, low carbon dioxide compensation point, low photorespiration rate, and high photosynthetic intensity. 90% of the planting area of ​​sugarcane is distributed in Between the Tropic of Cancer and the South. my country is the earliest country in the world to grow sugar cane, with a history of about 2,500 years. Sugar cane has a high biological yield and a large profit, and is the main raw mate...

AI Technical Summary

Problems solved by technology

In addition, bagasse is mainly used as fuel for steam boilers, as the main raw material for making low-density and medium-density fiberboards, etc., and the antioxidant components in it are discarded, and the resources are not fully utilized.

As mentioned above, the research on the effective active ingredients in sugarcane only involves the micro-analysis detection, physical and chemical properties or preliminary activity research of one of the active ingredients, and most of them only stay at the stage of theoretical research. The extraction, separation and purification of active ingredients, especially the comprehensive extraction, separation and purification of active ingredients in sugarcane have not been reported in the literature.