Method for extracting beta- glucosans from bran of corn

A technology of glucan and bran, which is applied to the separation and extraction of β-glucan, the separation and extraction process of β-glucan, and the grain field, which can solve the problems of increasing solvent residues, low purity of β-glucan, and obtaining Low efficiency and other problems, to achieve the effect of improving purity, improving economic benefits, social benefits, and high purity

Active Publication Date: 2009-10-14
TIBET TIANMAILI HEALTH PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since ethanol will be brought in during this process, on the one hand, it will increase the solvent residue, and on the other hand, because ethanol has no selectivity to the polysaccharide in the solution, the purity of the prepared β-glucan is not high, and the yield is low
In addition, since ethanol is a flammable and explosive product, there are many requirements for the production environment, which will increase the production cost of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the process of extracting β-glucan from highland barley bran

[0022] (1) The germ of the highland barley grains is completely removed, the bran is mechanically ground, and then the bran is separated and passed through a 100-mesh sieve.

[0023] (2) Mix the highland barley bran and water at a mass ratio of 1:8 to 1:10, and stir evenly; then adjust the pH value to 9 to 10 with 3mol / L sodium hydroxide solution, Extract under temperature conditions for half an hour; then add high-temperature α-amylase with a bran quality of 2‰~3‰ and extract for another half an hour to inactivate the enzyme; use a horizontal screw discharge sedimentation centrifuge for separation: the speed is 2000~3000 rpm points, the processing capacity is 0.2m 3 ~3m 3 .

[0024] (3) Adjust the pH of the separated supernatant to 2.2-3.5 with 1 mol / L hydrochloric acid, and after natural sedimentation for 12 hours, separate with a horizontal screw discharge sedimentation centrifuge, and di...

Embodiment 2

[0028] Embodiment 2, the process of extracting β-glucan from highland barley bran

[0029] (1) The germ of the highland barley grains is completely removed, the bran is mechanically ground, and then the bran is separated and passed through 100 sieves.

[0030] (2) Mix the highland barley bran and water at a mass ratio of 1:10 to 1:15, and stir evenly; adjust the pH value to 10 to 11 with 3mol / L sodium hydroxide solution, Extract under the same conditions for half an hour; then add high-temperature α-amylase with a bran quality of 3‰~5‰ and extract for another half an hour to inactivate the enzyme; use a horizontal screw discharge sedimentation centrifuge for separation: the speed is 3000~5000 rpm , the processing capacity is 0.2m 3 ~3m 3 .

[0031] (3) Adjust the pH of the separated supernatant to 4.0-5.0 with 1 mol / L hydrochloric acid, let it settle naturally for 20 hours, and separate it with a horizontal screw discharge sedimentation centrifuge, discarding the solid prot...

Embodiment 3

[0035] Embodiment 3, the process of extracting β-glucan from highland barley bran

[0036] (1) The germ of the highland barley grains is completely removed, the bran is mechanically ground, and then the bran is separated and passed through 100 sieves.

[0037] (2) Mix highland barley bran and water at a mass ratio of 1:15, stir evenly; use 3mol / L sodium hydroxide solution to adjust the pH value to 10, and extract at 45°C for half an hour; then add bran The high-temperature α-amylase with a quality of 5‰ was extracted for another half an hour to inactivate the enzyme; it was separated with a horizontal screw discharge sedimentation centrifuge at a speed of 5000 rpm.

[0038] (3) Adjust the pH of the separated supernatant to 3.5 with 1mol / L hydrochloric acid, and settle naturally? hour, then use a horizontal screw discharge sedimentation centrifuge to separate (rotating speed 5000 rev / min), discard the solid protein part.

[0039](4) The supernatant after separating and discar...

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PUM

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Abstract

The invention provides a method for extracting beta- glucosans by taking bran of corn as material. The method comprises the bran of corn is mixed with water and then extracted respectively by alkali liquor and amylase for centrifugal separation; supernate is added with acid for precipitation to separate and remove protein and concentrated by a spiral-wound film; the mixed solution is precipitated by ammonium sulphate solution, and the precipitated product is dissolved in the water and then concentrated by the spiral-wound film and dried to obtain highland barley beta- glucosans. A horizontal spiral unloading sedimentation centrifugal device is adopted to effectively improve the yield of the beta- glucosans; the ammonium sulphate solution is adopted to selectively sedimentate the beta- glucosans, so as to effectively remove other heteroglycans such as pentosan, xylan and the like in the material, and effectively improve the purity of the beta- glucosans; by adopting the spiral-wound film concentration technique, the water-quality standard of a great deal of produced industrial sewage is higher than tap water, and the industrial sewage can be recycled, so that the aim of energy-saving and emission-reduction can be achieved, and the economic benefit and social benefit of the enterprise can be improved.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a separation and extraction process of β-glucan, in particular to a method for separating and extracting β-glucan from grain bran. Mainly suitable for barley, highland barley, oats, wheat and other grains. Background technique [0002] The chemical name of β-glucan is: (1-3)(1-4)-β-D-glucan, which is a non-starch polysaccharide. Studies have shown that β-glucan has the functions of regulating blood sugar, improving immunity, and anti-tumor. At present, the biomedical and nutritional circles generally believe that the physiological effects of β-glucan mainly include four aspects: bowel cleansing, blood sugar regulation, cholesterol lowering, and immunity enhancement. [0003] According to the information, the grains of highland barley / oat / naked oats contain a lot of β-glucan, among which the content of β-glucan in highland barley is about 3.5-8.6%; the content of β-gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12S3/02
Inventor 雷菊芳郝勇王義
Owner TIBET TIANMAILI HEALTH PROD CO LTD
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