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Molecular marker auxiliary selection method for TYLCV breeding

A yellow leaf curl virus, molecular marker-assisted technology, applied in biochemical equipment and methods, microbial determination/inspection, etc. The effect of improved screening efficiency, high efficiency and cost, easy operation

Inactive Publication Date: 2009-10-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to aim at the traditional method of combining resistance identification and plant phenotype in tomato disease resistance breeding with large workload, slow process, long cycle, easy to be restricted by environmental conditions, low breeding efficiency, high cost, etc. In the breeding process of tomato resistance to yellow leaf curl virus disease, it is used for early isolation generation and seedling stage disease resistance identification, which can accurately, quickly and efficiently select genetic materials containing resistance to yellow leaf curl virus disease Molecular marker assisted selection method

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  • Molecular marker auxiliary selection method for TYLCV breeding
  • Molecular marker auxiliary selection method for TYLCV breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: (Utilize molecular marker assisted selection method to the resistance detection 1 of hybrid progeny material)

[0027] The specific implementation steps in this example are:

[0028] 1. Parent material and hybridization method:

[0029] ①Parent material:

[0030] Excellent tomato line T01-198: bred and preserved by Vegetable Research Institute of Zhejiang Academy of Agricultural Sciences. The Netherlands introduced a hybrid generation 'Tomato Serrei';

[0031] 07-026: Contains resistance gene Ty-3 against tomato yellow leaf curl virus disease, provided by Asian Vegetable Research and Development Center (AVRDC);

[0032] 07-027: Contains resistance gene Ty-2 against tomato yellow leaf curl virus disease, provided by Asian Vegetable Research and Development Center (AVRDC);

[0033] ② Hybridization method:

[0034] With T07-026 as the female parent and T01-198 as the male parent, it was awarded F 1 Hybrid combination 07-026×T01-198; then use T01-198 as ...

Embodiment 2

[0046] Embodiment 2: (Utilize molecular marker assisted selection method to the resistance detection 2 of hybrid progeny material)

[0047] The specific implementation steps in this example are:

[0048] 1. Parent material and hybridization method:

[0049] ①Parent material:

[0050] 9179 is a stable strain selected from highly segregated offspring after 8 generations of strains after crossing the first-generation hybrid variety 9502 introduced from Europe with high resistance to leaf mold and 9132 introduced by the Asian Vegetable Research and Development Center. Infinity, pink, large fruit, resistant to leaf mold, susceptible to tomato yellow leaf curl virus;

[0051] 07-026 and 07-027 materials are the same as in Example 1;

[0052] ② Hybridization method:

[0053] With 9179 as the female parent and 07-027 as the male parent, it was awarded F 1 The hybrid combination 9179×07-027; the F with 07-026 as the male parent 1 Hybridization was carried out to obtain the three-...

Embodiment 3

[0057] Embodiment 3: (application of artificial seedling stage inoculation identification method to the verification of molecular marker-assisted selection of resistant materials)

[0058] The resistance homozygous and heterozygous band types of screening markers were used to select resistant plants. During the breeding process of tomato resistance to yellow leaf curl virus disease, double PCR technology was used to simultaneously detect Ty-2 and Ty-3 genes, and to screen Material containing resistance gene 07-026×T01-198BC 1 -6, 07-026×T01-198BC 1 -86, 07-026×T01-198BC 1 -112, (2698BC 1 -6×07-027)F 2 -53, (2698BC 1 -6×07-027)F 2 -72, ((9179×07-027)×07-026)F 2 -88, ((9179×07-027)×07-026)F 2 -105, etc., and then further applied the artificial seedling inoculation identification method to verify the molecular marker-assisted selection resistant plants (Table 1).

[0059] Table 1 The incidence of the resistant material TYLCV whitefly after inoculation

[0060] res...

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Abstract

The invention discloses a molecular marker auxiliary selection method for TYLCV breeding, and belongs to the technical field of vegetable disease resistance breeding. The method comprises the following steps: (1) extracting DNA from breeding separation generation materials; (2) sieving SCAR molecular markers of anti-TYLCVD genes; (3) performing double PCR amplification and electrophoretic analytic detection on the DNA; and (4) evaluating the resistance of the breeding separation generation materials according to the detection result. The method can accurately and quickly detect whether any generation material in the breeding process is polymerized with the anti-TYLCV genes in a laboratory so as to obviously shorten the period of disease resistance breeding and reduce the breeding scale. Moreover, the method has the advantages of simple and convenient operation, time conservation, labor conservation, cost reduction and the like, reduces the workload, improves the sieving efficiency of resistant materials, accelerates the disease resistance breeding process, and can be popularized and applied to tomato breeding units.

Description

technical field [0001] The invention belongs to the technical field of vegetable disease-resistant breeding, in particular to a molecular marker-assisted selection method for tomato yellow leaf curl virus disease-resistant breeding. Background technique [0002] Tomato (Lycopersicon esculentum Mill.) is an important vegetable crop in the world. It has many varieties, high yield, rich nutrition and wide application. The main reasons for limiting the development and yield of tomato production are the prevalence of diseases and the restriction of adverse conditions. Tomato yellow leaf curl virus disease (TYLCVD) is one of the important diseases that limit tomato production. The disease is caused by tomato yellow leaf curl virus (Tomato yellow leaf curl virus) curl virus, TYLCV) and spread by whitefly (Bemisia tobaci), as early as the 1860s, Israel and other countries have reported. With the change of global climate, the change of agricultural farming system, the rapid streng...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 叶青静杨悦俭王荣青周国治阮美颖李志邈姚祝平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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