Karaya gum complex polysaccharide microsphere used as bioactive molecular vector and preparation method thereof

A technology of biologically active molecules, karaya gum, applied in the direction of being fixed on/in an organic carrier, can solve the problem of not involving the preparation method and application of microspheres, poor mechanical properties and poor stability of biological polysaccharide microspheres and other problems, to achieve the effect of simplifying the production operation process, wide application range and abundant resources

Active Publication Date: 2009-10-28
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned biological polysaccharide microspheres have poor mechanical properties, poor stability in acid-base solution, complicated producti

Method used

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  • Karaya gum complex polysaccharide microsphere used as bioactive molecular vector and preparation method thereof
  • Karaya gum complex polysaccharide microsphere used as bioactive molecular vector and preparation method thereof
  • Karaya gum complex polysaccharide microsphere used as bioactive molecular vector and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. get a certain amount of karaya gum and join in the sodium hydroxide aqueous solution that massfraction is 40%, the sodium hydroxide aqueous solution and karaya gum ratio are volume (ml): mass (g)=10: 1, add simultaneously Sodium tetraborate, the mass ratio of karaya gum to sodium tetraborate is 5:1, keep the temperature in a water bath at 60°C for 22 hours, then neutralize the excess alkali with glacial acetic acid with a concentration of 90%, and use a dialysis bag with a molecular weight cut-off of 8000-10000 Dialysis in flowing distilled water at room temperature for 48 hours to remove excess salt, vacuum drying at 50°C and 0.08 MPa for 68 hours to obtain deacetylated karaya gum dry powder, with a degree of deacetylation of 86%;

[0028] Step 2. the karaya gum of deacetylation is mixed with chitosan, and mass ratio is 1: 1, joins in distilled water under 20 ℃, 1000rpm / min rotating speed (containing the glacial acetic acid that volume ratio is 1.0% in the aqueo...

Embodiment 2

[0033]Step 1. get a certain amount of karaya gum and join in the sodium hydroxide aqueous solution that massfraction is 40%, the sodium hydroxide aqueous solution and karaya gum ratio are volume (ml): mass (g)=11: 1, add simultaneously Sodium tetraborate, the mass ratio of karaya gum to sodium tetraborate is 4:1, keep the temperature in a water bath at 60°C for 24 hours, then neutralize the excess alkali with 90% glacial acetic acid, and use a dialysis bag with a molecular weight cut-off of 8000-10000 Dialysis in flowing distilled water at room temperature for 48 hours to remove excess salt, vacuum drying at 50°C and 0.08MPa for 73 hours to obtain deacetylated karaya gum dry powder, with a degree of deacetylation of 85%;

[0034] Step 2. the karaya gum of deacetylation is mixed with chitosan, and mass ratio is 1.2: 1, joins in distilled water under 22 ℃, 1000rpm / min rotating speed (containing the glacial acetic acid that volume ratio is 1.3% in the aqueous solution), Obtaining...

Embodiment 3

[0039] Step 1. get a certain amount of karaya gum and join in the sodium hydroxide aqueous solution that mass fraction is 40%, the sodium hydroxide aqueous solution and karaya gum ratio are volume (ml): mass (g)=12: 1, add simultaneously Sodium tetraborate, the mass ratio of karaya gum to sodium tetraborate is 3:1, keep the temperature in a water bath at 60°C for 26 hours, then neutralize the excess alkali with 90% glacial acetic acid, and use a dialysis bag with a molecular weight cut-off of 8000-10000 Dialysis in flowing distilled water at room temperature for 48 hours to remove excess salt, vacuum drying at 50°C and 0.08MPa for 76 hours to obtain deacetylated karaya gum dry powder, with a degree of deacetylation of 82%;

[0040] Step 2. the karaya gum of deacetylation is mixed with chitosan, and mass ratio is 1.3: 1.1, joins in distilled water under 25 ℃, 1000rpm / min rotating speed (containing the glacial acetic acid that volume ratio is 1.6% in the aqueous solution), Obtai...

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Abstract

The invention relates to a karaya gum complex polysaccharide microsphere used as a bioactive molecular vector and a preparation method thereof. The prior microsphere has poor mechanical property, poor stability in acid or alkali solution, complicated production processes and high cost. The karaya gum complex polysaccharide microsphere uses a compound of biological polysaccharide karaya gum (deacetylation) and chitosan as a raw material and glutaric dialdehyde as a cross-linking agent to carry out polymerization; the karaya gum complex polysaccharide microsphere with -CHO and -COOH surface functional groups is prepared, the surface of the microsphere is in a poriferous honeycomb structure, and the microsphere has the grain diameter from 0.6*10 microns to 2.1*10 microns, the mechanical strength from 4.6N to 8.12N/grain and the swelling coefficient from 0.67ml/g to 0.93ml/g and is stable in the environment with the pH value from 1 to 14. The karaya gum complex polysaccharide microsphere has simple production technology and good biocompatibility, does not have toxic side effects and can be used as a good vector of enzyme or other bioactive molecules.

Description

technical field [0001] The invention relates to the field of enzymes and other active molecular carriers, especially karaya gum composite polysaccharide microspheres. Background technique [0002] Karaya Gum Composite Polysaccharide Microspheres are acid and alkali resistant polymer microspheres that are porous inside and have special functional groups on the surface. [0003] The preparation method of polymer microspheres has been paid great attention by researchers at home and abroad. And disclosed by various patents, such as: Japanese patent 2554835 applied for a preparation method of oil-water emulsion microspheres; Japanese patent 1649922 applied for cross-linked polymers, polymer particles and their preparation methods; French patent 10102303 applied for carbon dioxide Silicon microspheres, their preparation and aggregation methods and possible applications of silica microspheres; US Patent 1226927 applied for the coagulation preparation method of ionically cross-link...

Claims

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Application Information

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IPC IPC(8): C08L5/00C08L5/08C08J3/12C12N11/10
Inventor 张继杜兆莉魏铄蕴王云普张生堂许生军白志强杨丽
Owner NORTHWEST NORMAL UNIVERSITY
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