Three fungus strains for promoting germination of arethusa seeds
A technology of plant seeds and fungi, applied in the field of bioengineering, can solve the problems of limited popularization and application, no large-scale cultivation base, lack of systematic research, etc., and achieve the effect of huge application value
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Embodiment 1
[0022] Isolation of germination fungi
[0023] Seeds are sown in a suitable growth environment of Bletilla striata and Dendrobium to obtain seeds, protocorms and seedlings, and the germination fungi are separated and screened after disinfecting the germination material surface.
Embodiment 2
[0025] Effects of Bsg14 on the germination of Dendrobium candidum seeds
[0026] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal hyphae. Take the sterilized wheat bran, leaves and sawdust as the control, and cultivate in the culture room at a culture temperature of 23-27 ℃, 12 hours of light per day, light intensity: 1200Lx, two months to investigate the seed germination, the result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 67.3%, the seed germination of Dendrobium candidum without the fungus treatment The rate is 0.0%.
Embodiment 3
[0028] Effects of Bsg14 on the germination of Dendrobium candidum seeds
[0029] Sow the seeds of Dendrobium officinale on wheat bran, leaves and sawdust that have been covered with Bsg14 fungal hyphae, and use sterilized wheat bran, leaves and sawdust as the control, cultivate in a greenhouse at a temperature of 15-26℃ , Natural light, the daily light time is 8-10 hours, and the seed germination was investigated at two months. The result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 65.1%, and the seed germination of Dendrobium candidum treated without fungus treatment The rate is 0.0%.
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