Three fungus strains for promoting germination of arethusa seeds

A technology of plant seeds and fungi, applied in the field of bioengineering, can solve the problems of limited popularization and application, no large-scale cultivation base, lack of systematic research, etc., and achieve the effect of huge application value

Inactive Publication Date: 2009-10-28
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical application, when the aseptic seedlings obtained by tissue culture rapid propagation are used for cultivation, the two links of training seedlings and transplanting not only limit the popularization and application of this method in terms of technology but also production costs.
Before a breakthrough in technology in this area, it is still difficult to achieve large-scale cultivation and production of Dendrobium plants.
[0007] There are only scattered reports about the cultivation techniques of Bletilla striata, but there is a lack of systematic research, and there is no large-scale cultivation base. There are still many problems to be solved in the traditional method of asexual reproduction of wild plants.
Wild mutated species need a long domestication process, but so far there a

Method used

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  • Three fungus strains for promoting germination of arethusa seeds
  • Three fungus strains for promoting germination of arethusa seeds
  • Three fungus strains for promoting germination of arethusa seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation of germination fungi

[0023] Seeds are sown in a suitable growth environment of Bletilla striata and Dendrobium to obtain seeds, protocorms and seedlings, and the germination fungi are separated and screened after disinfecting the germination material surface.

Embodiment 2

[0025] Effects of Bsg14 on the germination of Dendrobium candidum seeds

[0026] Sow the seeds of Dendrobium candidum on the wheat bran, leaves and sawdust that have been covered with Bsg14 fungal hyphae. Take the sterilized wheat bran, leaves and sawdust as the control, and cultivate in the culture room at a culture temperature of 23-27 ℃, 12 hours of light per day, light intensity: 1200Lx, two months to investigate the seed germination, the result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 67.3%, the seed germination of Dendrobium candidum without the fungus treatment The rate is 0.0%.

Embodiment 3

[0028] Effects of Bsg14 on the germination of Dendrobium candidum seeds

[0029] Sow the seeds of Dendrobium officinale on wheat bran, leaves and sawdust that have been covered with Bsg14 fungal hyphae, and use sterilized wheat bran, leaves and sawdust as the control, cultivate in a greenhouse at a temperature of 15-26℃ , Natural light, the daily light time is 8-10 hours, and the seed germination was investigated at two months. The result is: the seed germination rate of Dendrobium candidum treated with Bsg14 fungus: 65.1%, and the seed germination of Dendrobium candidum treated without fungus treatment The rate is 0.0%.

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Abstract

The invention discloses three fungus strains for promoting the germination of arethusa seeds. The classification names of the three fungus strains are respectively Rhizoctonia sp. Bsg11 with the preservation serial number of CGMCC No.2450, Tulasnella sp. Bsg14 with the preservation serial number of CGMCC No.2451 and Mycena sp. MF24 with the preservation serial number of CGMCC No.1350. The three effective fungus strains have an important meaning for solving the germination problem of arethusa seeds; and the three effective fungus strains can be seeded together with the arethusa seeds, are used for the cultivation of the cultured seedlings or the large fields of a seedling culture disk or a flowerpot or a seedbed and have an application value for replacing tissue culture to obtain seedlings.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to three fungal strains that promote the germination of orchid plant seeds, which can be sown with orchid plant seeds and used for seedling raising or field cultivation in seedling trays, flower pots or seedbeds. Background technique [0002] There are many traditional and valuable Chinese medicines in orchid plants, such as dendrobium and bletilla striata. According to the 2005 edition of the Chinese Pharmacopoeia, Dendrobium has the effects of nourishing the stomach, nourishing fluid, nourishing yin and clearing away heat, and can also improve the throat and eyesight, and regulate the immune function of the human body. Bletilla striata is used as a tuber as medicine. It has the effects of astringent and hemostasis, swelling and muscle growth, etc. It mainly treats hemoptysis and vomiting, traumatic bleeding, sore swelling toxin, skin chapped, pulmonary tuberculosis, hemoptysis, ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 郭顺星房慧勇陈晓梅肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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