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Fusant bacterial strain and applications thereof

A fusion technology of bacterial strains and protoplasts, applied in the direction of application, bacteria, and fungicides, can solve the problems of alfalfa main root rot, ineffective control of soil-borne alfalfa diseases, and restrictions on the use of chemical pesticides, so as to improve quality, promote yield, The effect of improving stress resistance

Inactive Publication Date: 2011-09-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The second is that the root system and taproot of alfalfa are rotted due to Fusarium spp., which seriously affects the production of alfalfa.
The research results show that the use of chemical pesticides is seriously restricted due to the characteristics of grassland itself and factors such as economy, environment and food safety.
At the same time, chemical control methods cannot effectively control the occurrence of soil-borne alfalfa diseases (Leath, et.al.1971)

Method used

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  • Fusant bacterial strain and applications thereof
  • Fusant bacterial strain and applications thereof
  • Fusant bacterial strain and applications thereof

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Embodiment 1

[0020] Embodiment 1, the acquisition of fusion son F17 CGMCC No.2885

[0021] 1) Preparation of protoplasts from Sinorhizobia NX2004062CGMCC No.2883

[0022] 1. Isolation of Sinorhizobium sp. NX2004062CGMCC No.2883

[0023] a. Isolation of strains

[0024] Method: From the alfalfa experimental field in Beibao, Luhua Town, Xixia District, Ningxia City, alfalfa plants growing vigorously above the ground were collected, and the nodules on the roots were observed. The pink nodules were picked and placed in small sampling bottles. Anhydrous sodium sulfate was placed at the bottom as a desiccant. After taking the root nodules back indoors, wash them three times with sterile water, disinfect the surface with 3% sodium hypochlorite for 5 minutes, and then wash them once with sterile water, then transfer the root nodules to sterilized absorbent paper, and absorb the water. Put the root nodule in a sterilized mortar and smash it, dip it in its juice with an inoculation loop, and use ...

Embodiment 2、F17

[0072] Disease prevention and nitrogen fixation effect of embodiment 2, F17 CGMCC No.2885

[0073] 1) Antibacterial effect of F17 CGMCC No.2885

[0074] The Oxford cup method was used to determine the antibacterial effect of Bacillus subtilis HL259 CGMCC No.1451 and fusion F17 CGMCC No.2885 on 12 plant pathogenic fungi and 6 plant pathogenic bacteria. The 12 kinds of plant pathogenic fungi are Cladosporium fulvum, Botrytis cinerea, Valsa mali, Physalospora piricola, Curvularia lunata), Verticillium dahliae, F.oxysporum f.sp.vasinfectum, Rhizoctonia cerealia, Piricularia oryzae, Fusarium solani ( Fusarium solani), Fusarium avenaceam and Alternaria solani. The six strains of plant pathogenic bacteria were potato soft rot (Erwinia carotovora), root cancer (Agrobacterium rhizogene), cucumber angular spot (Pseudomonas syringae), cotton angular spot (Xanthomonas malvacearum), tomato canker (Clavibacter michiganensis subsp.Michiganensis, referred to as Cmm), Ralstonia solanacearum...

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Abstract

The invention discloses a fusant bacterial strain and applications thereof. The fusant bacterial strain is a fusant which is obtained by the fusing of the protoplast of bacillus subtilis HL 259 CGMCC No. 1451 and the protoplast of sinorhizobium sp. NX 2004062 CGMCC No. 2883, with the preservation number of CGMCC No. 2885. The fusant bacterial strain CGMCC No. 2885 provides effective measures for achieving high-yield and high-quality pasture grass industry and has significant scientific meaning and application value for raising purple medic output, improving the quality of purple medic and enhancing the stress resistance of purple medic and the like. The invention plays a significant role in sustainable agricultural development, agroecological construction of base and development of regional economy.

Description

technical field [0001] The invention relates to a fusion bacterial strain and its application. Background technique [0002] Alfalfa among leguminous plants is known as the king of forage. With the development of ecological agriculture and the adjustment of industrial structure, as well as the pull of market demand for forage products at home and abroad, the cultivation of alfalfa-based forage has shown a trend of rapid development, and Gradually develop in the direction of scale and intensification. However, two problems in the production of alfalfa seriously restricted the improvement of its yield and quality to a certain extent. One is due to the symbiotic effectiveness between rhizobia and its host, screening suitable rhizobia strains to inoculate alfalfa varieties can obtain better nitrogen fixation effect. The second is that the root system and taproot of alfalfa are rotted due to Fusarium spp., which seriously affects the production of alfalfa. The research results...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21A01N63/02A01P1/00A01P3/00A01P21/00C12R1/41C12R1/125
Inventor 刘西莉卢志军李健强贾小红刘鹏飞朱书生罗来鑫黄中乔
Owner CHINA AGRI UNIV
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