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Separation method of lysine bacteria

A separation method, lysine technology, applied to the separation of microorganisms, etc., can solve the problems of large equipment investment and high process energy consumption, and achieve the effect of high equipment investment, low equipment investment and low price

Active Publication Date: 2009-11-18
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of method that can effectively separate lysine thalline, this method can overcome the shortcoming of high energy consumption of the traditional process, equipment investment is big, achieves effective separation lysine thalline and lysine fermentation broth clean liquid purpose

Method used

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  • Separation method of lysine bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Materials and equipment:

[0023] Equipment and material name

model

quantity

Remark

centrifugal pump

Flow 100L

1 set

Inorganic flocculant

polyferric sulfate

500g

organic flocculant

Anionic

500g

Molecular weight 10 million polypropylene

Amide resin

Lysine Fermentation Broth

-----

250L

Workshop offers concentration 15%

concentrated sulfuric acid

industrial grade

5kg

Workshop offers

Plate and frame filter press

---

1 set

Filtration area 0.25 square meters

[0024] 2. Specific methods and results:

[0025] 2.1 Put 250L of fermentation broth into the reaction tank, adjust the pH value to 3.7 with concentrated sulfuric acid, heat and sterilize, the sterilization condition is 70°C and keep it warm for 1.5 hours;

[0026] 2.2 Prepare the inorganic flocculant with water to make a solution with a concentration o...

Embodiment 2

[0035] 1. Materials and equipment:

[0036] Equipment and material name

model

quantity

Remark

centrifugal pump

Flow 100L

1 set

Inorganic flocculant

PAC

500g

organic flocculant

Anionic

500g

Polyacrylamide with a molecular weight of 5 million

Amine resin

Lysine Fermentation Broth

-----

250L

Workshop offers

Concentration 14.1%

concentrated sulfuric acid

industrial grade

4kg

workshop offers

Plate and frame filter press

---

one

Filtration area 0.25 square meters

[0037] 2. Specific methods and results:

[0038] 2.1 Put 250L of fermentation broth into the reaction tank, adjust the pH value to 4.8 with concentrated sulfuric acid, heat and sterilize, the sterilization condition is 65°C and keep it warm for 2.5 hours;

[0039] 2.2 Prepare the inorganic flocculant with water to make a solution with a concentr...

Embodiment 3

[0048] 1. Materials and equipment:

[0049] Equipment and material name

model

quantity

Remark

centrifugal pump

Flow 100L

1 set

Inorganic flocculant

PAC

500g

organic flocculant

Anionic

500g

Molecular weight 15 million polypropylene

Amide resin

Lysine Fermentation Broth

-----

250L

Workshop offers

Concentration 13.5%

concentrated sulfuric acid

industrial grade

Workshop offers

Plate and frame filter press

---

1 set

Filtration area 0.25 square meters

[0050] 2. Specific methods and results:

[0051] 2.1 Put 250L of fermentation broth into the reaction tank, adjust the pH value to 5.6 with concentrated sulfuric acid, heat and sterilize, the sterilization condition is 70°C and keep it warm for 2 hours;

[0052] 2.2 Prepare the inorganic flocculant with water to make a solution with a concentration of 2%; ...

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Abstract

The invention relates to a separation method of lysine bacteria, comprising the following steps: 1) adding assistant agents to fermentation liquor and mixing for 1 to 60 minutes; 2) pressing the fermentation liquor treated with the assistant agents into a plate and frame filter press for filtering. The used assistant agents comprise inorganic polymeric ferric and poly-aluminum assistant agents, and organic anionic assistant agents. Compared with the current common separation methods in the prior art, the invention has the advantages of simple and practical technique, low consumption of water, electricity and steam, low equipment investment, low maintenance cost, low moisture content and high yield of the obtained bacteria.

Description

technical field [0001] The invention relates to the field of amino acid chemical industry, in particular to a method for separating lysine bacteria. Background technique [0002] Lysine is one of the eight amino acids that the human body cannot synthesize by itself but is very much needed. Because of the lack of lysine in our country's staple food: cereals, it is also called "the first essential amino acid". Adding lysine to beverages, rice, flour, canned food and other foods can increase the utilization rate of protein, thereby greatly strengthening the nutrition of food, promoting growth and development, increasing appetite, reducing diseases, and enhancing physical fitness; moreover, Lysine is an excellent food fortifier, which can deodorize and keep fresh in cans; therefore, lysine is widely used in feed and food industries. [0003] Lysine is mainly prepared by fermentation, and the solid-liquid separation of the fermentation liquid is an important link in the producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
Inventor 周永生顾宗池王勇满云
Owner COFCO BIOTECHNOLOGY CO LTD
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