Production method of additive of bait for photosynthetic bacteria aquaculture

Abstract

The invention relates to a production method of an additive of a bait for photosynthetic bacteria aquaculture, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heatdisinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the additive of the bait for photosynthetic bacteria aquaculture under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r / m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the additive has the advantages of stable product quality and diversified functions.

Description

technical field

[0001] The invention relates to the field of microorganisms, in particular to a production method of a photosynthetic bacteria aquaculture bait additive. Background technique

[0002] With the rapid development of industry and agriculture in our country and the improvement of people's living standards, a large amount of untreated industrial and agricultural production wastewater and domestic sewage are directly discharged; coupled with the improvement of the degree of intensification of aquaculture in our country, the pollution of aquaculture waters in our country is becoming more and more serious. serious. The resulting diseases of farmed aquatic animals have increased in a large number and occur frequently. The blind and frequent use of fish (veterinary) medicines, antibiotics, hormones, etc. has seriously threatened the safety of aquatic products. In order to protect the fishery ecological environment, increase the amount and output of aquatic products, ...

Examples

Embodiment 1

[0037] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 6.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 30°C, and inoculate into the bacteria The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with 5% seed medium volume was anaerobically cultured for 3 days at a temperature of 15°C and a light intensity of 1000lux to obtain a reddish-brown strain culture solution containing The amount of bacteria reaches 5X10 8 cfu / ml.

[0038] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microorganism fermenter, sterilize at 121°C for 60 minutes, and when the medium is cooled to 30°C, produce the medium by fermentation 5% of the volume of the inoculum was inserted into the culture medium of the bacteria. The temperature is 30°C; the stirring speed i...

Embodiment 2

[0040] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 8.5 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 35°C, and inoculate the strains The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with a medium volume of 20% was anaerobically cultured at a temperature of 35°C and a light intensity of 3000lux for 4 days to obtain a reddish-brown strain culture solution containing bacteria The amount reaches 8X10 8 cfu / ml.

[0041] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microbial fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 35°C, produce the medium by fermentation 40% of the volume of the inoculum into the culture medium. The temperature is 30°C; the stirring speed is 300 rpm; the tank pressure is ...

Embodiment 3

[0043] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 7.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 32°C, and inoculate into the bacteria The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with a volume of 15% of the seed medium was anaerobically cultured for 4 days at a temperature of 25°C and a light intensity of 2000lux to obtain a reddish-brown strain culture solution containing The amount of bacteria reaches 8X10 8 cfu / ml.

[0044] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microbial fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 32°C, produce the medium according to the fermentation method 15% of the volume of the inoculum into the culture medium. The temperature is 29°C; the stirri...

technical field

[0001] The invention relates to the field of microorganisms, in particular to a production method of a photosynthetic bacteria aquaculture bait additive. Background technique

[0002] With the rapid development of industry and agriculture in our country and the improvement of people's living standards, a large amount of untreated industrial and agricultural production wastewater and domestic sewage are directly discharged; coupled with the improvement of the degree of intensification of aquaculture in our country, the pollution of aquaculture waters in our country is becoming more and more serious. serious. The resulting diseases of farmed aquatic animals have increased in a large number and occur frequently. The blind and frequent use of fish (veterinary) medicines, antibiotics, hormones, etc. has seriously threatened the safety of aquatic products. In order to protect the fishery ecological environment, increase the amount and output of aquatic products, ...

Examples

Embodiment 1

[0037] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 6.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 30°C, and inoculate into the bacteria The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with 5% seed medium volume was anaerobically cultured for 3 days at a temperature of 15°C and a light intensity of 1000lux to obtain a reddish-brown strain culture solution containing The amount of bacteria reaches 5X10 8 cfu / ml.

[0038] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microorganism fermenter, sterilize at 121°C for 60 minutes, and when the medium is cooled to 30°C, produce the medium by fermentation 5% of the volume of the inoculum was inserted into the culture medium of the bacteria. The temperature is 30°C; the stirring speed i...

Embodiment 2

[0040] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 8.5 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 35°C, and inoculate the strains The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with a medium volume of 20% was anaerobically cultured at a temperature of 35°C and a light intensity of 3000lux for 4 days to obtain a reddish-brown strain culture solution containing bacteria The amount reaches 8X10 8 cfu / ml.

[0041] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microbial fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 35°C, produce the medium by fermentation 40% of the volume of the inoculum into the culture medium. The temperature is 30°C; the stirring speed is 300 rpm; the tank pressure is ...

Embodiment 3

[0043] Step 1: Dissolve the various components of the above strain expansion medium in distilled water and put them into a glass bottle, adjust the pH to 7.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, cool to 32°C, and inoculate into the bacteria The mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaericus with a volume of 15% of the seed medium was anaerobically cultured for 4 days at a temperature of 25°C and a light intensity of 2000lux to obtain a reddish-brown strain culture solution containing The amount of bacteria reaches 8X10 8 cfu / ml.

[0044] Step 2: Dissolve the various components of the above-mentioned fermentation medium in tap water and put them into a fully automatic photosynthetic microbial fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 32°C, produce the medium according to the fermentation method 15% of the volume of the inoculum into the culture medium. The temperature is 29°C; the stirri...