Production method of additive of bait for photosynthetic bacteria aquaculture

Abstract

The invention relates to a production method of an additive of a bait for photosynthetic bacteria aquaculture, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heatdisinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the additive of the bait for photosynthetic bacteria aquaculture under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r / m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the additive has the advantages of stable product quality and diversified functions.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a production method of a photosynthetic bacteria aquaculture bait additive. Background technique [0002] With the rapid development of industry and agriculture in our country and the improvement of people's living standards, a large amount of untreated industrial and agricultural production wastewater and domestic sewage are directly discharged; coupled with the improvement of the degree of intensification of aquaculture in our country, the pollution of aquaculture waters in our country is becoming more and more serious. serious. The resulting diseases of farmed aquatic animals have increased in a large number and occur frequently. The blind and frequent use of fish (veterinary) medicines, antibiotics, hormones, etc. has seriously threatened the safety of aquatic products. In order to protect the fishery ecological environment, increase the amount and output of aquatic products, ...

AI Technical Summary

Problems solved by technology

This animal feed additive is a liquid preparation produced by photosynthetic bacteria, which is also used in aquaculture, but because its production raw materials use 10-40% starch aqueous solution, the nutrients are not comprehensive, and it is difficult to meet the nitrogen source required by the growth of photosynthetic bacteria , phosphorus source, sulfur source and other large elements, so the growth is poor

At the same time, the starch aqueous solution used in this patent is saccharified, and the acidic fermentation liquid is obtained by natural acidification. Since the acidification bacteria are not artificially controlled, the quality of the acidic fermentation liquid is difficult to guarantee

Moreover, there is no sterilization treatment before inoculation of photosynthetic bacteria strains, so the acidic fermentation liquid contains a large number of heterotrophic bacteria, which will inhibit the growth of photosynthetic bacteria, thereby causing serious pollution of the product, and the pollution rate can reach 80%. above, resulting in the instability of the application effect