Production method of additive of bait for photosynthetic bacteria aquaculture
A technology for photosynthetic bacteria and aquaculture, applied in the field of microorganisms, can solve the problems of unstable application effect, poor growth, product pollution, etc., and achieve the effect of improving the utilization rate of bait, balanced nutrient elements, and obvious effect.
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[0036] Example 1
[0037] Step 1: Dissolve the various components of the above-mentioned strain expansion medium in distilled water and put it into a glass bottle, adjust the pH to 6.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, and when it is cooled to 30°C, inoculate the bacteria A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a medium volume of 5%, cultured anaerobically for 3 days at a temperature of 15°C and a light intensity of 1000 lux, to obtain a red-brown bacterial culture medium containing The amount of bacteria reaches 5X10 8 cfu / ml.
[0038] Step 2: Dissolve the various components of the above fermentation medium in tap water and put it into a fully automatic photosynthetic microorganism fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 30°C, produce the medium according to fermentation The inoculum volume of 5% of the volume is connected to the bacterial culture solution. The temperatur...
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[0039] Example 2
[0040] Step 1: Dissolve the various components of the above-mentioned bacteria expansion medium in distilled water and put them into glass bottles, adjust the pH to 8.5 with sodium hydroxide, sterilize at 121℃ for 30 minutes, and inoculate the bacteria when cooled to 35℃ A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a medium volume of 20% is cultured anaerobically for 4 days at a temperature of 35°C and a light intensity of 3000 lux to obtain a red-brown bacterial culture medium containing bacteria The amount reaches 8X10 8 cfu / ml.
[0041] Step 2: Dissolve the various components of the above fermentation medium in tap water and put them into a fully automatic photosynthetic microorganism fermentor, sterilize at 121°C for 60 minutes, and when the medium is cooled to 35°C, produce the medium according to fermentation 40% of the volume of the inoculum is connected to the culture broth. The temperature is 30℃; the stirring speed is ...
Example Embodiment
[0042] Example 3
[0043] Step 1: Dissolve the various components of the above-mentioned strain expansion medium in distilled water and put it into a glass bottle, adjust the pH to 7.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, and when it is cooled to 32°C, inoculate the bacteria A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a volume of 15% of the culture medium is cultured anaerobically for 4 days at a temperature of 25°C and a light intensity of 2000 lux to obtain a red-brown bacterial culture medium containing The amount of bacteria reaches 8X10 8 cfu / ml.
[0044] Step 2: Dissolve the various components of the above fermentation medium in tap water and put it into a fully automatic photosynthetic microorganism fermentor. Sterilize at 121°C for 60 minutes. When the medium is cooled to 32°C, produce the medium according to fermentation An inoculum of 15% of the volume is connected to the bacterial culture solution. The temperature ...
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