Production method of additive of bait for photosynthetic bacteria aquaculture

A technology for photosynthetic bacteria and aquaculture, applied in the field of microorganisms, can solve the problems of unstable application effect, poor growth, product pollution, etc., and achieve the effect of improving the utilization rate of bait, balanced nutrient elements, and obvious effect.

Active Publication Date: 2009-12-09
SHUNDE HONGLONG BIOLOGY SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This animal feed additive is a liquid preparation produced by photosynthetic bacteria, which is also used in aquaculture, but because its production raw materials use 10-40% starch aqueous solution, the nutrients are not comprehensive, and it is difficult to meet the nitrogen source required by the growth of photosynthetic bacteria , phosphorus source, sulfur source and other large elements, so the growth is poor
At the same time, the starch aqueous solution used in this patent is saccharified, and the acidic fermentation liquid is obtained by na

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] Step 1: Dissolve the various components of the above-mentioned strain expansion medium in distilled water and put it into a glass bottle, adjust the pH to 6.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, and when it is cooled to 30°C, inoculate the bacteria A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a medium volume of 5%, cultured anaerobically for 3 days at a temperature of 15°C and a light intensity of 1000 lux, to obtain a red-brown bacterial culture medium containing The amount of bacteria reaches 5X10 8 cfu / ml.

[0038] Step 2: Dissolve the various components of the above fermentation medium in tap water and put it into a fully automatic photosynthetic microorganism fermentation tank, sterilize at 121°C for 60 minutes, and when the medium is cooled to 30°C, produce the medium according to fermentation The inoculum volume of 5% of the volume is connected to the bacterial culture solution. The temperatur...

Example Embodiment

[0039] Example 2

[0040] Step 1: Dissolve the various components of the above-mentioned bacteria expansion medium in distilled water and put them into glass bottles, adjust the pH to 8.5 with sodium hydroxide, sterilize at 121℃ for 30 minutes, and inoculate the bacteria when cooled to 35℃ A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a medium volume of 20% is cultured anaerobically for 4 days at a temperature of 35°C and a light intensity of 3000 lux to obtain a red-brown bacterial culture medium containing bacteria The amount reaches 8X10 8 cfu / ml.

[0041] Step 2: Dissolve the various components of the above fermentation medium in tap water and put them into a fully automatic photosynthetic microorganism fermentor, sterilize at 121°C for 60 minutes, and when the medium is cooled to 35°C, produce the medium according to fermentation 40% of the volume of the inoculum is connected to the culture broth. The temperature is 30℃; the stirring speed is ...

Example Embodiment

[0042] Example 3

[0043] Step 1: Dissolve the various components of the above-mentioned strain expansion medium in distilled water and put it into a glass bottle, adjust the pH to 7.0 with sodium hydroxide, sterilize at 121°C for 30 minutes, and when it is cooled to 32°C, inoculate the bacteria A mixed culture of Rhodopseudomonas palustris and Rhodobacter sphaeroides with a volume of 15% of the culture medium is cultured anaerobically for 4 days at a temperature of 25°C and a light intensity of 2000 lux to obtain a red-brown bacterial culture medium containing The amount of bacteria reaches 8X10 8 cfu / ml.

[0044] Step 2: Dissolve the various components of the above fermentation medium in tap water and put it into a fully automatic photosynthetic microorganism fermentor. Sterilize at 121°C for 60 minutes. When the medium is cooled to 32°C, produce the medium according to fermentation An inoculum of 15% of the volume is connected to the bacterial culture solution. The temperature ...

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Abstract

The invention relates to a production method of an additive of a bait for photosynthetic bacteria aquaculture, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heatdisinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the additive of the bait for photosynthetic bacteria aquaculture under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r/m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the additive has the advantages of stable product quality and diversified functions.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a production method of a photosynthetic bacteria aquaculture bait additive. Background technique [0002] With the rapid development of industry and agriculture in our country and the improvement of people's living standards, a large amount of untreated industrial and agricultural production wastewater and domestic sewage are directly discharged; coupled with the improvement of the degree of intensification of aquaculture in our country, the pollution of aquaculture waters in our country is becoming more and more serious. serious. The resulting diseases of farmed aquatic animals have increased in a large number and occur frequently. The blind and frequent use of fish (veterinary) medicines, antibiotics, hormones, etc. has seriously threatened the safety of aquatic products. In order to protect the fishery ecological environment, increase the amount and output of aquatic products, ...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/20A23K1/18A23K10/18A23K50/80
Inventor 张肇铭黄振江吕俭雄李琦何锦霞潘广驹罗根周超南
Owner SHUNDE HONGLONG BIOLOGY SCI & TECH
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